iTaq Universal SYBR Green Supermix

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iTaq Universal SYBR Green Supermix

Overview

Superior Gene Expression Analysis
Superior Gene Expression Analysis

iTaq™ universal SYBR® Green supermix provides reliable gene expression data. The human β-actin gene was amplified from HeLa cDNA (100 ng–100 fg) using a CFX96™ real-time PCR detection system. iTaq™ universal SYBR® Green supermix produced greater than 90% efficiency over 6 orders of linear dynamic range. Standard curve R2 = 0.999, β-actin efficiency = 92.6%, slope =
–3.51. RFU, relative fluorescence units.

Sensitive and Efficient Amplification of Genomic DNA

iTaq™ Universal SYBR® Green Supermix allows robust amplification of genomic DNA.

The human GAPDH gene was amplified from human genomic DNA (50 ng–5 pg) using a CFX96™ real-time PCR detection system. iTaq Universal SYBR® Green Supermix produced a GAPDH efficiency of 108.1% over several orders of linear dynamic range. Standard curve R2 = 0.993, slope = –3.14. RFU, relative fluorescence units.



Consistent Results across Real-Time Instrument Platforms
Consistent Results Across Platforms

 

Sensitive and efficient gene expression analysis on any real-time PCR instrument. The β-actin gene was amplified from HeLa cDNA (100 ng–100 fg) using iTaq™ universal SYBR® Green supermix on several real-time instruments. A, Bio-Rad® CFX96™ real-time PCR detection system; B, Bio-Rad® iQ™5 real-time PCR detection system; C, ABI 7900 real-time PCR system; D, ABI 7500 fast real-time PCR system; E, ABI ViiA7 real-time PCR system; F, ABI StepOnePlus real-time PCR system. This supermix showed excellent β-actin efficiencies, R2, and slopes on all platforms (>90% efficiency, R2 > 0.990, slope between –3.20 and –3.58). RFU, relative fluorescence units.

Provides unmatched specificity and reaction efficiency for both genomic DNA and cDNA targets in qPCR on any real-time PCR instrument.
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Description

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iTaq Universal SYBR Green Supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based quantitative PCR (qPCR) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl2, SYBR Green I dye, enhancers, stabilizers, and a blend of passive reference dyes (including ROX and fluorescein).

iTaq Universal SYBR Green Supermix lets you:

iTaq Universal SYBR Green Supermix contains:

  • Advanced buffer formulation with SYBR Green I for optimal qPCR performance
  • Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
  • Blend of passive reference dyes for universal real-time instrument compatibility

Instrument Compatibility

iTaq Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
BioFire
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses

  • Gene expression analysis
  • Genotyping
  • Mutation detection
  • Pathogen detection


Specifications

Storage at –20°C Guaranteed for 12 months protected from light in a constant temperature freezer
Repeated freeze/thaw cycles Up to 10 times
Storage at 4°C Guaranteed for 3 months protected from light
Polymerase iTaq DNA polymerase
Hot-start mechanism Antibody mediated
Hot-start activation 95°C, 30 sec
Supermix concentration 2x
Thermal cycling protocol Standard and fast
Instrument compatibility Validated on all Bio-Rad and ROX-dependent Applied Biosystems real-time PCR instruments and Roche LightCycler 480, QIAGEN Rotor-Gene Q, Eppendorf Mastercycler ep realplex, and Stratagene Mx real-time PCR systems
Template compatibility cDNA, genomic DNA, plasmid DNA
Detection method SYBR® Green

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iTaq™ Universal SYBR® Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725120
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes

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$157.00 
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iTaq™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725121
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX Normalization Dyes

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$332.00 
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iTaq™ Universal SYBR® Green Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725122
1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes

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$636.00 
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iTaq™ Universal SYBR® Green Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)

1725124
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes

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$1,469.00 
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iTaq™ Universal SYBR® Green Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)

1725125
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX normalization dyes

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$2,785.00 
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Documents

Number Description Options
10000068167 iTaq™ Universal SYBR® Green Supermix Product Insert, Ver A Click to download
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
7178 Amplification: Real-Time PCR Reagents and Kits Brochure Click to download
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier Click to download

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