iTaq Universal Probes Supermix

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iTaq Universal Probes Supermix

Overview

Duplex qPCR under Challenging Conditions
Duplex qPCR under Challenging Conditions

Duplex qPCR under challenging conditions. cDNA from human liver (100 ng) was used in each 20 µl reaction to amplify GAPDH (VIC-labeled probe) and IL-2 (FAM-labeled probe) in simplex (, tubulin; , IL-2) and duplex () reactions using iTaq™ universal probes supermix on a CFX96™ real-time PCR detection system. There are no significant differences in quantification cycle (Cq) values in the detection of a low-expressing gene (IL-2) in a simplex or duplex reaction in the presence of a high-expressing gene (GAPDH). RFU, relative fluorescence units.

Reliable and Reproducible Gene Expression Analysis

iTaq™ Universal Probes Supermix is excellent for gene expression analysis.

Gene Expression Analysis

The human β-actin gene was amplified from HeLa cDNA (100 ng–100 fg) using FAM-labeled probes on a CFX96™ real-time PCR detection system. iTaq Universal Probes Supermix produced a β-actin efficiency of 94.3% over 6 orders of linear dynamic range. Standard curve R2 = 0.999, slope = –3.47. RFU, relative fluorescence units.



Consistent Results across Real-Time Instrument Platforms
Consistent Results Across Platforms

 

Sensitive and efficient gene expression analysis on any real-time PCR instrument. The β-actin gene was amplified from HeLa cDNA (100 ng–100 fg) using iTaq™ universal probes supermix and FAM-labeled probes on several real-time instruments. A, Bio-Rad® CFX96™ real-time PCR detection system; B, Bio-Rad® iQ™5 real-time PCR detection system; C, ABI 7900 real-time PCR system; D, ABI 7500 fast real-time PCR system; E, ABI ViiA7 real-time PCR system; F, ABI StepOnePlus real-time PCR system. This supermix showed excellent β-actin efficiencies, R2, and slopes on all platforms (>90% efficiency, R2 > 0.990, slope between –3.20 and –3.58). RFU, relative fluorescence units.

Allows challenging simplex and duplex qPCR for a wide variety of gene expression analysis targets on any real-time PCR instrument.
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Description

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iTaq universal probes supermix is a 2x concentrated, ready-to-use reaction master mix containing all components, except primers, probes, and template, for probe-based quantitative PCR (qPCR; simplex or duplex reaction) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl2, enhancers, stabilizers, and a blend of passive reference dyes (including ROX).

iTaq universal probes supermix lets you:

iTaq universal probes supermix contains:

  • Advanced buffer formulation for optimal probe-based qPCR
  • Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
  • Blend of passive reference dyes for universal real-time instrument compatibility

Instrument Compatibility

iTaq Universal Probes Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
BioFire
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses

  • Gene expression analysis
  • Genotyping
  • Mutation detection
  • Pathogen detection


Specifications

Storage at –20°C Guaranteed for 12 months protected from light in a constant temperature freezer
Repeated freeze/thaw cycles Up to 10 times
Storage at 4°C Guaranteed for 3 months protected from light
Polymerase iTaq DNA polymerase
Hot-start mechanism Antibody mediated
Hot-start activation 95°C, 30 sec
Supermix concentration 2x
Thermal cycling protocol Standard and fast
Instrument compatibility Validated on all Bio-Rad and ROX-dependent Applied Biosystems real-time PCR instruments and Roche LightCycler 480, QIAGEN Rotor-Gene Q, Eppendorf Mastercycler ep realplex, and Stratagene Mx real-time PCR systems
Template compatibility cDNA, genomic DNA, plasmid DNA
Detection method Fluorogenic probes

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iTaq™ Universal Probes Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725130
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes

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$157.00 
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iTaq™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725131
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, enhancers, stabilizers, and ROX Normalization Dyes

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$332.00 
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iTaq™ Universal Probes Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725132
1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes

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$636.00 
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iTaq™ Universal Probes Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)

1725134
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes

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$1,469.00 
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iTaq™ Universal Probes Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)

1725135
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes

List Price: 
 
$2,785.00 
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Documents

Number Description Options
10000068969 iTaq™ Universal Probes Supermix Product Insert, Ver A Click to download
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
7178 Amplification: Real-Time PCR Reagents and Kits Brochure Click to download
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier Click to download

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