iScript Select cDNA Synthesis Kit

Print
iScript Select cDNA Synthesis Kit

Overview

The iScript™ Select Kit Demonstrates Superior Fidelity
The iScript Select Kit Demonstrates Superior Fidelity

The iScript Select Kit demonstrates superior fidelity when compared to several other commonly used reverse transcriptases. A partial fragment of the ampicillin resistance gene was reverse transcribed using one of six different commonly used reverse transcriptases according to manufacturer’s instructions. PCR was performed using Bio-Rad’s iProof™ High-Fidelity Master Mix. Resulting amplicons were sequenced using the Illumina MiSeq platform. For each sample, 10 million bases of valid reads with three or more repeats were obtained. Fidelity of each reverse transcriptase was determined by taking the inverse of the error rate. Mean accuracy of four replicates is shown. Error bars are standard errors of four replicates.

Long cDNA Synthesis

The iScript Select cDNA synthesis kit facilitates synthesis of cDNA longer than 6 kb. First-strand cDNA was produced from 1 µg total RNA using the iScript Select cDNA synthesis kit and the provided oligo(dT) primer mix. A 2 µl aliquot of the 20 µl cDNA synthesis reaction was subjected to 35 PCR cycles using a proofreading high-fidelity polymerase and human adenomatous polyposis coli (APC) primer sets. From a 50 µl PCR reaction, 10 µl was analyzed on a 1% agarose gel. Lane 1, 1 kb ladder; lanes 2 and 3, 6.4 kb APC PCR product; lanes 4 and 5, 8.2 kb APC PCR product.

Broad Dynamic Range

The iScript™ Select cDNA synthesis kit performs reliably over six orders of magnitude using any first-strand priming approach. Human total RNA from 1 pg to 1 µg was reverse transcribed using the iScript Select cDNA synthesis kit and the first-strand primers indicated above. The resulting cDNA was amplified in triplicate using iQ™ SYBR® Green supermix with β-actin primers and detected using the iCycler iQ® real-time detection system, RFU, relative fluorescence units.

A high-fidelity reverse transcription kit that also provides flexibility in primer setup (individual tubes of oligo(dT) and random primers).
Read full description

Ordering Information


Description

The iScript Select cDNA Synthesis Kit is a high-fidelity, sensitive reverse transcription system that provides ultimate flexibility. Unlike our other iScript cDNA Synthesis products, the reaction components for this kit are packaged in individual tubes, enabling flexible priming options and customization of reaction setup for specific application needs.

Key Features and Benefits

  • Achieve high-fidelity — unique reverse transcription kit minimizes introduction of base pair errors
  • Synthesize long cDNAs — efficiently synthesize cDNA greater than 8 kb
  • Save time and reduce errors during setup — less hands on time and less pipetting steps compared to protocols for other high-fidelity reverse transcriptase kits
  • Detect low-level target genes and conserve RNA — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
  • Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription


High-Fidelity

iScript Select cDNA Synthesis Kit is designed for applications requiring high-fidelity, such as cloning and NGS. This kit introduces fewer base pair errors than other commonly used reverse transcription kits.

Ideal RT for Many Applications

The combination of iScript Select Kit performance and its packaging of various priming options in individual tubes enables this reagent to perform optimally across many different applications.

A versatile reverse transcription kit serves all of your RT-PCR needs

  • Cloning

    Cloning

  • RT-qPCR

    RT-qPCR

  • NGS

    NGS

  • Sequencing

    Sequencing

Selector Tools

Use our selectors to find the best reagents, plates, tubes, seals, and accessories for your PCR and qPCR experiments.


Choose the best cDNA synthesis kit for your application, with formulations optimized for ease, yield, flexibility, or gDNA clearance.


Specifications

Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature
freezer (multiple freeze thaws not recommended)

Ordering

x

Account price failed to display. Please try reloading the page.Reload Page

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
email to

iScript™ Select cDNA Synthesis Kit, 25 x 20 µl rxns

1708896
25 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

List Price: 
 
$170.00 
LoadingLoading...
EDU Price:
 
What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
iScript™ Select cDNA Synthesis Kit, 100 x 20 µl rxns

1708897
100 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

List Price: 
 
$523.00 
LoadingLoading...
EDU Price:
 
What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
iScript™ Select cDNA Synthesis Kit, 500 x 20 µl rxns

1708897BUN
500 x 20 µl reactions, includes 5x reaction mix, iScript Reverse Transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

List Price: 
 
$2,461.00 
LoadingLoading...
EDU Price:
 
What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 

Documents

Number Description Options
10001023 Instruction Manual, iScript™ Select cDNA Synthesis Kit, Rev E Click to download
7075 iScript™ Select cDNA Synthesis Kit Flier Click to download
7076 iScript™ Select cDNA Synthesis Kit Has Highest Fidelity as Determined by Next-Generation Sequencing Click to download
7037 iScript High-Fidelity Cloning Applications Protocol Click to download
6746 iScript Reverse Transcription Kits Flier Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier Click to download
5211 iProof High-Fidelity DNA Polymerase Flier, Rev C Click to download [ Add to Cart (Free) ]
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Get Help
Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.