iScript™ cDNA Synthesis Kit

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iScript cDNA Synthesis Kit

Overview

Broad Dynamic Range

The iScript™ cDNA synthesis kit performs across a broad range of concentrations. Input RNA (amounts shown in inset) was reverse transcribed and the resulting cDNA was amplified using iQ™ SYBR® Green supermix. The iScript cDNA synthesis kit performs over six orders of magnitude with high sensitivity. Standard curve r = 0.998, efficiency = 96.5%, RFU, relative fluorescence units.

Excellent Sensitivity

Sensitivity of the iScript™ cDNA synthesis kit in RT-PCR using low amounts of input RNA. cDNA was synthesized at 42°C and amplified with iTaq™ DNA polymerase using β-actin primers. M, EZ Load™ 100 bp molecular ruler; C, no-template negative control.

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The iScript cDNA synthesis kit is a sensitive and easy-to-use first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. This two-tube kit is optimized to yield sensitive, unbiased representation over a broad dynamic range, with minimal setup and reaction time.

Key Features and Benefits

  • Have ease of use and consistency — 2-tube (iScript reaction mix and iScript reverse transcriptase) format for faster setup
  • Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
  • Utilize dilute RNA samples — reaction mix and iScript enzyme blend format allows up to 15 µl of RNA sample in a 20 µl cDNA synthesis reaction
  • Prevent 5' and 3' bias of your target genes and design primers in any region — optimum blend of oligo(dT) and random primers in the 5x supermix
  • Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription
  • Complete cDNA synthesis and qPCR on the same day — fast 26 minute cDNA synthesis protocol
Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
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iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns

1708890
25 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

0 1708890 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8890_0921120538440.xml 1708890 09/21/09 12:05 PM en_US true internet/product_SKU 2019-05-04 16:19:12 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8890_0921120538440.xml /default/main/bio-rad/WORKAREA/default jcun051o 2017-11-14 07:07:38 true jcun051o templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8890_0921120538440.xml LSR N /default/main/bio-rad en 09/21/22 12:05 PM 1555975165477 2019-05-04 16:19:12 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8890_0921120538440.xml 1708890-iscript-cdna-synthesis-kit-25-x-20-ul-rxns 1 1708890 iScript cDNA Synthesis Kit iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns 25 x 20 &#181;l reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water 25 x 20 &#181;l reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water 25 x 20 &#181;l reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8890_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8890_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/170-8890_feature.jpg ,VN RepEx 170-8890 25 x 20 &#181;l reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water 170-8890, 1708890, iscript, cdna, synthesis, reverse, transcription, first, strand, complimentary, gene, expression, real, time, qpcr, detection 09/21/09 12:05 PM 09/21/22 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Real-Time-PCR-Detection-System N 0 <p>Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Up to 15 &micro;l of RNA in a 20 &micro;l cDNA synthesis reaction</li> <li>Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions</li> <li>Fast 26 min cDNA synthesis protocol</li> <li>Linear range of 1 &micro;g&ndash;1 pg total input RNA using RNase H+ MMLV reverse transcriptase</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, &micro;l</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;25</td> <td style="padding-left: 70px;" valign="top">&nbsp; 100 (1 x 100 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1708890</span></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp; 100</td> <td style="padding-left: 70px;" valign="top">&nbsp; 400 (1 x 400 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1708891-iscript-cdna-synthesis-kit-100-x-20-ul-rxns"><span style="font-size: 11px;">1708891</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp; 500</td> <td style="padding-left: 70px;" valign="top">&nbsp; 2,000 (5 x 400 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1708891bun-iscript-cdna-synthesis-kit-500-x-20-ul-rxns"><span style="font-size: 11px;">1708891BUN</span></a></td> </tr> </tbody> </table> <br /><br /> <p>See the <a href="/en-us/product/iscript-cdna-synthesis-kit">iScript cDNA Synthesis Kit</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li> <li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iScript cDNA Synthesis Kit <a href="/webroot/web/pdf/lsr/literature/4106228.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated/ 170-8890_0921120538440_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//170-8890_0921120538440_en.html 1708842 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8842_1301949358.xml HSP9601 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Plastic-Consumables/HSP-9601_0921120534821.xml MSC1001 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Plastic-Consumables/MSC-1001_1297906204.xml false $165.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708890&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708890&CartQuote=QT&quantity= 1445 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708890&AddToDefaultWishList=true&quantity= 41105900 165.00 Product detail page 1708890
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iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns

1708891
100 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

1 1708891 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8891_0921120538190.xml 1708891 09/21/09 12:05 PM en_US true internet/product_SKU 2019-05-04 16:19:27 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8891_0921120538190.xml /default/main/bio-rad/WORKAREA/default jcun050q 2017-11-14 07:07:38 true jcun050q templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8891_0921120538190.xml LSR N /default/main/bio-rad en 12/31/37 11:59 PM 1556922204059 2019-05-04 16:19:27 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8891_0921120538190.xml 1708891-iscript-cdna-synthesis-kit-100-x-20-ul-rxns 1 1708891 iScript cDNA Synthesis Kit iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns 100 x 20 &#181;l reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water 100 x 20 &#181;l reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water 100 x 20 &#181;l reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8891_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8891_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/170-8891_feature.jpg ,VN RepEx 170-8891 100 x 20 &#181;l reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water 170-8891, 1708891, iscript, cdna, synthesis, reverse, transcription, first, strand, complimentary, gene, expression, real, time, qpcr, detection 09/21/09 12:05 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Real-Time-PCR-Detection-System N 0 <p>Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Up to 15 &micro;l of RNA in a 20 &micro;l cDNA synthesis reaction</li> <li>Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions</li> <li>Fast 26 min cDNA synthesis protocol</li> <li>Linear range of 1 &micro;g&ndash;1 pg total input RNA using RNase H+ MMLV reverse transcriptase</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, &micro;l</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;25</td> <td style="padding-left: 70px;" valign="top">&nbsp; 100 (1 x 100 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1708890-iscript-cdna-synthesis-kit-25-x-20-ul-rxns"><span style="font-size: 11px;">1708890</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp; 100</td> <td style="padding-left: 70px;" valign="top">&nbsp; 400 (1 x 400 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1708891</span></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp; 500</td> <td style="padding-left: 70px;" valign="top">&nbsp; 2,000 (5 x 400 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1708891bun-iscript-cdna-synthesis-kit-500-x-20-ul-rxns"><span style="font-size: 11px;">1708891BUN</span></a></td> </tr> </tbody> </table> <br /><br /> <p>See the <a href="/en-us/product/iscript-cdna-synthesis-kit">iScript cDNA Synthesis Kit</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li> <li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iScript cDNA Synthesis Kit <a href="/webroot/web/pdf/lsr/literature/4106228.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated/ 170-8891_0921120538190_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//170-8891_0921120538190_en.html 1708842 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8842_1301949358.xml HSP9601 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Plastic-Consumables/HSP-9601_0921120534821.xml MSC1001 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Plastic-Consumables/MSC-1001_1297906204.xml false $481.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708891&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708891&CartQuote=QT&quantity= 1445 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708891&AddToDefaultWishList=true&quantity= 41105900 481.00 Product detail page 1708891
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iScript™ cDNA Synthesis Kit, 500 x 20 µl rxns

1708891BUN
500 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

1708891BUN /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8891BUN_1318355597.xml 1708891BUN 10/11/11 10:44 AM en_US true internet/product_SKU 2019-05-04 16:19:06 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8891BUN_1318355597.xml /default/main/bio-rad/WORKAREA/default jcun05hm 2017-11-14 07:07:36 true jcun05hm templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8891BUN_1318355597.xml LSR N /default/main/bio-rad en 10/11/21 10:44 AM 1555974589295 2019-05-04 16:19:06 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8891BUN_1318355597.xml 1708891bun-iscript-cdna-synthesis-kit-500-x-20-ul-rxns 1 1708891BUN iScript cDNA Synthesis Kit (500 reactions) iScript™ cDNA Synthesis Kit, 500 x 20 µl rxns 500 x 20 &micro;l reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water 500 x 20 &micro;l reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8891BUN_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8891BUN_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/170-8891BUN_feature.jpg ,VN Karen Moss iScript&trade; cDNA Synthesis Kit (500 reactions) 500 x 20 &micro;l reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water 170-8891BUN, 1708891BUN, iscript, cdna, synthesis, reverse, transcription, first, strand, complimentary, gene, expression, real, time, qpcr, detection, rt-qpcr 10/11/11 10:44 AM 10/11/21 10:44 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Up to 15 &micro;l of RNA in a 20 &micro;l cDNA synthesis reaction</li> <li>Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions</li> <li>Fast 26 min cDNA synthesis protocol</li> <li>Linear range of 1 &micro;g&ndash;1 pg total input RNA using RNase H+ MMLV reverse transcriptase</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td align="center" valign="top"><strong>Number of 20 &micro;l Reactions</strong></td> <td align="center" valign="top"><strong>Supermix Volume, &micro;l</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp;&nbsp;25</td> <td style="padding-left: 70px;" valign="top">&nbsp; 100 (1 x 100 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1708890-iscript-cdna-synthesis-kit-25-x-20-ul-rxns"><span style="font-size: 11px;">1708890</span></a></td> </tr> <tr> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp; 100</td> <td style="padding-left: 70px;" valign="top">&nbsp; 400 (1 x 400 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><a href="/en-us/sku/1708891-iscript-cdna-synthesis-kit-100-x-20-ul-rxns"><span style="font-size: 11px;">1708891</span></a></td> </tr> <tr class="pd_colorbackground"> <td style="padding-left: 80px;" valign="top">&nbsp;&nbsp; 500</td> <td style="padding-left: 70px;" valign="top">&nbsp; 2,000 (5 x 400 &micro;l)</td> <td style="padding-left: 40px;" valign="top"><span style="font-size: 11px;">1708891BUN</span></td> </tr> </tbody> </table> <br /><br /> <p>See the <a href="/en-us/product/iscript-cdna-synthesis-kit">iScript cDNA Synthesis Kit</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>View all <a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li> <li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iScript cDNA Synthesis Kit <a href="/webroot/web/pdf/lsr/literature/4106228.pdf">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global 170-8891BUN_desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/170-8891BUN_desc.html &nbsp; 1 0 false $2,262.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708891BUN&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708891BUN&CartQuote=QT&quantity= 1445 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708891BUN&AddToDefaultWishList=true&quantity= 41106300 2262.00 Product detail page 1708891BUN
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4106228 iScript™ cDNA Synthesis Kit Product Insert, Ver D Click to download
6746 iScript Reverse Transcription Kits Flier Click to download [ Add to Cart (Free) ]
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier Click to download

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