iScript™ cDNA Synthesis Kit

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iScript cDNA Synthesis Kit

Overview

Broad Dynamic Range

The iScript™ cDNA synthesis kit performs across a broad range of concentrations. Input RNA (amounts shown in inset) was reverse transcribed and the resulting cDNA was amplified using iQ™ SYBR® Green supermix. The iScript cDNA synthesis kit performs over six orders of magnitude with high sensitivity. Standard curve r = 0.998, efficiency = 96.5%, RFU, relative fluorescence units.

Excellent Sensitivity

Sensitivity of the iScript™ cDNA synthesis kit in RT-PCR using low amounts of input RNA. cDNA was synthesized at 42°C and amplified with iTaq™ DNA polymerase using β-actin primers. M, EZ Load™ 100 bp molecular ruler; C, no-template negative control.

Offers ease-of-use and a linear dynamic range of 1 µg to 100 fg. This two-component cDNA synthesis kit uses a 26-minute protocol.
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Description

The iScript cDNA synthesis kit is a sensitive and easy-to-use first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. This two-tube kit is optimized to yield sensitive, unbiased representation over a broad dynamic range, with minimal setup and reaction time.

Key Features and Benefits

  • Have ease of use and consistency — 2-tube (iScript reaction mix and iScript reverse transcriptase) format for faster setup
  • Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
  • Utilize dilute RNA samples — reaction mix and iScript enzyme blend format allows up to 15 µl of RNA sample in a 20 µl cDNA synthesis reaction
  • Prevent 5' and 3' bias of your target genes and design primers in any region — optimum blend of oligo(dT) and random primers in the 5x supermix
  • Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription
  • Complete cDNA synthesis and qPCR on the same day — fast 26 minute cDNA synthesis protocol

Specifications

Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)

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iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns

1708890
25 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

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$170.00 
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iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns

1708891
100 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

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$495.00 
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iScript™ cDNA Synthesis Kit, 500 x 20 µl rxns

1708891BUN
500 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water

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$2,330.00 
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Documents

Number Description Options
4106228 iScript™ cDNA Synthesis Kit Product Insert, Ver D Click to download
6746 iScript Reverse Transcription Kits Flier Click to download [ Add to Cart (Free) ]
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
5990 Gene Expression Analysis Solutions Brochure Click to download
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier Click to download

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