iScript Advanced cDNA Synthesis Kit for RT-qPCR

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iScript Advanced cDNA Synthesis Kit

Overview

Allows increased data throughput (high capacity up to 7.5 µg input RNA) from a single 20 µl reverse transcription reaction. This two-component cDNA synthesis kit features a short 21-minute protocol.
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Description

The iScript Advanced cDNA Synthesis Kit for RT-qPCR is an enhanced formulation that delivers increased data throughput from a single 20 µl reverse transcription (RT) reaction for real-time qPCR. This two-tube kit enables superior RT capacity of input RNA up to 7.5 µg.

Key Features and Benefits

  • Increase qPCR data throughput and cost effectiveness from a single reaction — cDNA synthesized from higher input RNA amounts can be used for analyzing large numbers of target genes
  • Detect low-level target genes — uncompromised sensitivity even with less input RNA for when RNA sample is limited
  • Reduce inter-assay variability and improve reproducibility — eliminate the need for multiple cDNA synthesis reactions
  • Allow true representation of target mRNA levels — potent blend of RNase inhibitor prevents RNA degradation during reaction setup and RT
  • Increase primer design flexibility and prevent 5′ and 3′ bias — optimum blend of oligo(dT) and random primers for superior RNA coverage
  • Set up reactions with ease and obtain cDNA in less time — 2-tube kit generates cDNA in 21 minutes

Specifications

Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)

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iScript™ Advanced cDNA Synthesis Kit, 25 x 20 µl rxns

1725037
25 x 20 µl reactions, includes 100 µl iScript Advanced 5x Reaction Mix, 25 µl iScript Advanced Reverse Transcriptase

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$214.00 
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iScript™ Advanced cDNA Synthesis Kit, 100 x 20 µl rxns

1725038
100 x 20 µl reactions, includes 400 µl 5x iScript Advanced Reaction Mix, 100 µl iScript Advanced Reverse Transcriptase

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$676.00 
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iScript™ Advanced cDNA Synthesis Kit, 500 x 20 µl rxns

1725038BUN
500 x 20 µl reactions, includes 5 x 400 µl 5x iScript Advanced Reaction Mix, 5 x 100 µl iScript Advanced Reverse Transcriptase

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$3,082.00 
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Documents

Number Description Options
10000070791 iScript™ Advanced cDNA Synthesis Kit for RT-qPCR Product Insert, Ver B Click to download
6746 iScript Reverse Transcription Kits Flier Click to download [ Add to Cart (Free) ]
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier Click to download
6777 Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR Click to download
6816 Protocol for Multiplex RT-qPCR Analysis Using PrimePCR™ Assays, Ver A Click to download
6833 High Throughput Single Cell Gene Expression Profiling by Multiplex qPCR, Ver A Click to download

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