Freeze 'N Squeeze™ DNA Gel Extraction Spin Columns

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Freeze 'N Squeeze DNA Gel Extraction Spin Columns

Overview

Freeze 'N Squeeze Method
Freeze 'N Squeeze Recovery

Recovery of DNA fragments using Freeze 'N Squeeze spin columns. Extracted fragments were rerun on a ReadyAgarose 1% TAE gel (lanes 2–6). Lane 1, precision molecular mass ruler; lane 7, EZ Load 100 bp PCR molecular ruler.

PCR Product and DNA Fragment Purification Guide

Bio-Rad offers a variety of pre-PCR and post-PCR sample preparation and cleanup columns and prepacked columns; many are also suitable for DNA fragment purification.

  Freeze 'N Squeeze DNA
Gel Extraction Spin Columns
Micro Bio-Spin and Bio-Spin Columns PCR Kleen
Spin Columns
Application Recovery of DNA fragments (50 bp–23 kb) purified from TAE- or TBE-buffered agarose gels Dye terminator and label cleanup from sequencing reactions; buffer exchange; desalting Removal of PCR products >200 bp from other reaction mixture components
Starting Material ≤700 mg gel slice 10–100 µl of sequencing reaction mixture 25–100 µl of PCR or other enzyme reaction
Format Spin-column extraction of frozen-thawed gel slices Prepacked size exclusion spin column Prepacked size exclusion spin column
Recovery 4–23 kb: >80% recovery;
0.5–4 kb: >70% recovery;
50–500 bp: >50% recovery
>95% of linear DNA >22 bases or bp; 99% retention of unincorporated dye terminators 50–80%
Time Required <10 min <10 min <4 min
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Freeze 'N Squeeze DNA gel extraction spin columns provide a quick and effective means to purify double-stranded DNA fragments from TAE- or TBE-buffered agarose gels. Unlike other purification methods which are often time-consuming or utilize toxic chaotropic materials, the Freeze 'N Squeeze spin column method purifies via filtration, using short centrifugation to draw 50–23,000 bp of DNA out of agarose gel slices that have been quickly frozen and thawed.

Key Features

  • Requires less than 1 minute of hands-on time and less than 10 minutes for DNA cleanup
  • No solutions to prepare
  • DNA immediately available for subsequent PCR, subcloning, ligations, and sequencing reactions
Applications Recovery of DNA fragments purified from TAE- or TBE-buffered agarose gels
Starting material ≤700 mg gel slice
DNA size 50–23,000 bp
Method Spin-column extraction of frozen-thawed gel slices
Recovery 4–23 kb: >80% recovery
Time required <10 min
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Freeze 'N Squeeze™ DNA Gel Extraction Spin Columns

7326165
Pkg of 25, columns for agarose gel DNA extraction, 50 bp–23 kb recovery

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Freeze 'N Squeeze™ DNA Gel Extraction Spin Columns

7326166
Pkg of 100, columns for agarose gel DNA extraction, 50 bp–23 kb recovery

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