EXQuest Spot Cutter

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EXQuest Spot Cutter


Discontinued Product
The ExQuest™ Spot Cutter has been discontinued. We will continue to provide support for ExQuest owners until March, 2020.

 

The EXQuest™ spot cutter is a precision instrument that accurately locates and excises protein bands or spots from 1-D and 2-D gels or blots. It then loads them into 96- and 384-well microplates or 96-tube racks for downstream processing and analysis. The EXQuest spot cutter is designed to be both robust in construction and simple to operate, reducing manual labor and improving throughput of protein spots for analysis.

The EXQuest spot cutter is a fully integrated system controlled through PDQuest™ 2-D analysis software or Quantity One® 1-D analysis software. It consists of an enclosure, an imaging system, a fluidics system, robotics, sensors, a cutting head, a gel tray, a microplate rack, and a wash station.

Versatile Excision Capability

The EXQuest spot cutter allows use of any common proteome separation and staining methods:

  • Freestanding 2-D and 1-D SDS-PAGE gels
  • Plastic- or glass-backed 2-D and 1-D gels
  • PVDF and nitrocellulose membrane blots
  • Gels or membranes stained for proteins with visible stains (such as silver and Coomassie blue stains) or fluorescent stains (such as Flamingo™ fluorescent gel stain and SYPRO Ruby protein stain)

Big Performance on the Bench

  • Accommodates any commercially available large or mini format gel or PVDF membrane blot in a small bench footprint
  • PDQuest software automatically selects spots in order from lowest to highest amount of protein, minimizing the chance of carryover contamination from spot to spot
  • Quantity One software provides convenient tools for 1–D gel cutting
  • Cutting tip produces precise cuts without damage or distortion on gels or PVDF membranes
  • Cuts up to 4 gels at a time at up to 600 spots per hour
  • System accuracy of ±0.1 mm ensures accurate excision of even small, closely grouped spots
  • Error-free data capture from image analysis to mass spectrometry
  • Built-in wash wells provide customized tip washing between cuts for extra contamination control
  • Quick-change settings to switch between UV and visible light modes
  • Compatible with ProteomeWorks™ and ProteomeWorks Plus spot cutters

 

System accuracy ±0.1 mm (100 µm)
Excision speed 600 spots per hour
Excision platform Accommodates one large gel (e.g., PROTEAN® Plus handcast gels) or up to four small gels (e.g., Criterion™ gels) without the need for trimming
Pickup rate >99.5% spot pickup on first cut
CCD camera 1,600 x 1,200 imaging pixel resolution, 100 µm resolution, 12-bit, cooled to 0°C
Excision tip nominal internal diameter 1.5 mm, 1.0 mm, and optional membrane cutting tip
Excision surfaces Backed or free gels 1-D and 2-D gels 0.5–2.0 mm thick; visible light applications (e.g., Coomassie blue, silver stain); UV light applications (e.g., Flamingo™, SYPRO Ruby); PVDF and nitrocellulose membrane blots
Operating temperature 15–30°C
Operating humidity range 12–85%, noncondensing
Dimensions (without computer) (W x D x H) 91 x 69 x 66 cm (35.8 x 27.2 x 26.0"). Requires 38 cm clearance above the front of the instrument and 25 cm clearance on left side of instrument
Weight Instrument only, 76 kg (168 lb)
Minimum computer requirements (computer not included) Microsoft Windows 2000 or XP operating system (1.2 GHz), 2.1 GB hard drive, 512 MB RAM, screen resolution 1,024 x 768, 2 MB video RAM
Connection type USB
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(Discontinued) EXQuest Spot Cutter With PC

1657201
Gel excision instrument, includes PC, enclosure, imaging system, fluidics system, robotics, sensors, cutting head, gel tray, microplate track, wash station

This product is no longer available. Please contact technical support for more information on replacement product.
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Cutting Tip

1657202
Pkg of 1, 1.0 mm cutting tip, for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
Cutting Tip

1657203
Pkg of 1, 1.5 mm cutting tip, for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) Glass Bottle

1657204
Pkg of 1, 1 L glass bottle for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is no longer available. Please contact technical support for more information on replacement product.
Calibration Pucks

1657205
Pkg of 10, calibration puck for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
Membrane Cutting Head

1657206
Pkg of 1, cutting head with 1.0 mm tip, for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
Membrane Cutting Tip

1657207
Pkg of 1, 1.0 mm cutting tip, for use with membrane cutting head (#165-7206) on EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
Gel Cutting Sheets

1657208
Pkg of 15, gel cutting sheet, for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
Gel Holding Clips

1657209
Pkg of 2, gel holding clip for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
Calibration Target

1657210
Pkg of 1, calibration target for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
Camera Target

1657211
Pkg of 1, camera target for use with EXQuest spot cutter (#165-7200, #165-7201)

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Micro Tubes

1657212
Pkg of 20, 1.5 ml, micro tube for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
Bottle Holder

1657214
Pkg of 1, bottle holder for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
Gel Tray

1657215
Pkg of 1, gel tray for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) Transilluminator Lamp

1657216
Pkg of 1, transilluminator lamp for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is no longer available. Please contact technical support for more information on replacement product.
Rounded-Bottomed Microplates

1657217
Pkg of 20, 96-well microplate, for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
Ferrule

1657218
Pkg of 10, 10-32, 1/16" OD, ferrule for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
Bar Code Reader

1657219
Pkg of 1, bar code reader for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) Microplate Holder

1657220
Pkg of 1, microplate holder for use with EXQuest spot cutter (#165-7200, #165-7201)

This product is no longer available. Please contact technical support for more information on replacement product.
PDQuest Advanced 2-D Analysis Software

1709630
1-user license, 2-D analysis software, provides advanced functionality

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Number Description Options
3194 EXQuest Spot Cutter Brochure, Rev A Click to download
4000129-14 Instruction Manual, The Discovery Series: PDQuest 2-D Analysis Software, Rev A Click to download
10001531 Instruction Manual, EXQuest™ Spot Cutter, Rev A Click to download
3097 Imaging and Analysis: Tools for Acquisition and Analysis of Protein Expression Data Brochure, Rev B Click to download

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