Description
Description
The BioLogic DuoFlow family of chromatography systems offers flexibility with multiple system configurations, many optional upgrades, and a common software platform that is intuitive and easy to follow. These systems can be used on the laboratory bench or in a coldroom.
The BioLogic DuoFlow Pathfinder 80 system includes:
- F40 workstation
- BioLogic Maximizer valve system for flow rates of up to 80 ml/min
- BioLogic QuadTec UV/Vis detector for simultaneous monitoring of four wavelengths ranging from 190–740 nm (with deuterium lamp at 190–370 nm and optional halogen lamp at 370–740 nm)
- BioLogic DuoFlow pH monitor for real-time monitoring of pH conditions during a run
- BioFrac fraction collector for the widest range of collection capabilities
A BioLogic Maximizer mixer, MX-1 mixer, 3-tray rack, AVR7-3 sample inject valve, fittings kit, system cable 25, conductivity monitor, starter kit, UNO Q1 column, diverter valve, two F1 racks, and instructions are also included.
The Dell PC controller enables easy communication with the workstation and peripheral devices via an external USB Bitbus communicator. The controller includes the Windows XP operating system, application software, keyboard, mouse, and high-resolution flat-panel monitor.
BioLogic DuoFlow modular components allow the system to meet both laboratory space and application requirements; as requirements change, systems may be easily reconfigured and seamlessly upgraded with increased functionality, such as higher flow rates, sophisticated detection capabilities, pH monitoring, column scouting, and buffer blending.
Specifications
Specifications
Specifications
BioLogic DuoFlow Systems
Operating Limits
Flow rate
40 ml/min
Pressure
1,000 psi (66 MPa) at 40 ml/min
Detection
UV Detection
Standard BioLogic QuadTec UV/Vis detector for simultaneous 4-wavelength detection
Conductivity
Standard
pH
Standard (BioLogic DuoFlow Maximizer)
Other
Optional signal import module (SIM) adds refractive index, and fluorescence detectors
Sample Loading
Large samples/
Optional SV5-4 valve, Model EP-1 Econo
buffer selection
pump or Econo gradient pump
Single sample
AVR7-3 valve
Multiple samples
Optional DynaLoop sample loops (25 ml)
Fraction Collection
General
BioFrac or Model 2110 fraction collector
Large-volume
BioFrac fraction collector with optional
Small-volume
BioFrac or Model 2110 fraction collector
Special Applications
Buffer blending
BioLogic Maximizer valve system (BioLogic DuoFlow Maximizer, BioLogic DuoFlow Pathfinder systems); optional in BioLogic DuoFlow, BioLogic QuadTec systems
Column scouting/
Optional AVR9-8 valves for .8 columns
column switching
or additional AVR7-3 valve for 2 columns
Reverse flow
Additional AVR7-3 valve
Solvent Compatibility
All commonly used chromatographic solvents
Operating temperature
4–40°C
Dimensions (W x D x H)
35 x 41 x 81 cm (13.8 x 16.1 x 31.9")
Weight
70 kg (154 lb)
Accessories
Accessories
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(Discontinued) 40 psi Backpressure Regulator
7500230
Pkg of 1, for use with BioLogic DuoFlow and NGC Workstations
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(Discontinued) F40 Pump Maintenance Kit
7600184
Kit includes 2 piston seals, 4 check valves, seal removal tool, 2 O-rings, to service one F40 pump
Image
(Discontinued) F40 Piston Kit
7600173
Kit, includes 2 pistons, to service one F40 pump
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(Discontinued) F40 Piston Seals
7600172
Pkg of 2, piston seals, and seal tool, to service one F40 pump
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(Discontinued) Check Valve
7500162
Pkg of 1, check valve, for use with BioLogic DuoFlow workstation, (4 check valves required per pump)
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(Discontinued) Inline Filter Kit
7500703
Filter kit, includes 1 inline filter and 2 replacement frits, for use with BioLogic DuoFlow and NGC Systems
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(Discontinued) F10 Pump Kit
7600110
Kit to convert an F40 workstation to an F10, includes 2 assembled pumpheads with seals and check valves, 4 piston assemblies, F10 tubing kit, tools; enables flow rates as low as 0.01 ml/min
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(Discontinued) BioLogic Maximizer Interconnect Tubing
7602003
Pkg of 2, high-pressure tubing, includes 2 PEEK prefitted tubing lengths for connecting BioLogic DuoFlow pumps to BioLogic Maximizer system
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(Discontinued) BioLogic Maximizer Tubing Kit
7602002
High-pressure tubing kit, includes 4 color coded, prefitted FEP tubing lengths for connecting solvent bottles to the BioLogic Maximizer system
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(Discontinued) F40 Tubing Kit
7600652
High-pressure tubing kit, includes precut and fitted PTFE, Tefzel, and green PEEK tubing, for installation of BioLogic DuoFlow basic chromatography system running at flow rates >40 ml/min
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(Discontinued) Bar Clamps
7500265
Pkg of 5, bar clamps, for use with BioLogic Rack (7500251)
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(Discontinued) Column Clamp Set
7500260
Column clamp set, includes 1 column clamp assembly, for use with BioLogic Rack (7500251)
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(Discontinued) Horizontal Bar Kit
7500264
Bar kit, includes 2 tie bars, 4 bar clamps, for use with BioLogic Rack (7500251)
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(Discontinued) Vertical Bars
7500263
Pkg of 2, 10 cm short bars, for use with BioLogic Rack (7500251)
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(Discontinued) Vertical Bars
7500262
Pkg of 2, 64 cm long bars, for use with BioLogic Rack (7500251)
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(Discontinued) BioLogic Rack Expansion Kit
7500268
Kit, includes 2 rack trays, 2 long vertical bars, 16 sleeves, for use with BioLogic Rack (7500251)
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(Discontinued) BioLogic Rack
7500251
Includes rack tray, 8 sleeves, 2 short vertical bars, 2 long vertical bars, column clamp set, 5 bar clamps, 4 cable organizers
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(Discontinued) DynaLoop 90 Kit
7500452
Sliding-piston sample loop kit, includes 90 ml DynaLoop sliding piston loop, DynaLoop parts kit (#750-0450)
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(Discontinued) DynaLoop 25 Kit
7500451
Sliding-piston sample loop kit, includes 25 ml DynaLoop sliding piston loop, DynaLoop parts kit (#750-0450)
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(Discontinued) Large-Volume Sample Loop Kit
7500491
Pkg of 1, sample loop kit, includes 1, 2, and 5 ml PEEK loops
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(Discontinued) Small-Volume Sample Loop Kit
7500490
Pkg of 1, sample loop kit, includes 100, 250, and 500 μl PEEK loops
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(Discontinued) Injection Needle
1250224
Pkg of 1, 22-gauge, injection needle, blunt, for use with BioLogic DuoFlow system
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(Discontinued) Sample Injection Port
7500471
Pkg of 1, sample injection port, for use with AVR7-3 automated sample inject valve (#760-0406)
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(Discontinued) BioLogic DuoFlow™ and NGC™ Fittings Kit
7600550
Fittings kit contains PEEK and Tefzel nuts, ferrules, unions, plugs, and luer syringe, for use with BioLogic DuoFlow and NGC systems
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(Discontinued) SVT3-2 Diverter Valve
7600410
Pkg of 1, solenoid valve, 3-port, 2-position, 30 psi (2 bar) limit, for use with BioLogic DuoFlow system
Documents
Documents
Login Required Number Description Options
DC7602030
Declaration of Conformity, BioLogic USB Bitbus Device, Rev A
2780
Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A
2849
Protein A Removal From IgG on CHT Ceramic Hydroxyapatite Support, Rev A
5342
Automated Three-Dimensional Purification Method for Histidine-Tagged Proteins Using the BioLogic DuoFlow Maximizer Chromatography System, Rev A
5334
A Method for Rapid, Large-Scale Removal of Albumin and IgG From Human Serum Using the BioLogic DuoFlow Chromatography System, Rev A
5251
Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins, Rev A
5926
Modular Protein Purification, Rev A
3039
Separation Reproducibility With the BioLogic Chromatography Systems
5326
Chromatography Column Connection Guide, Rev A
5369
Biologic DuoFlow System Family Brochure, Rev A
2584
BioLogic DuoFlow™ Valving Guide, Rev E
2815
BioLogic DuoFlow™ Valves Brochure, Rev C
2687
BioLogic DuoFlow Chromatography System Brochure, Rev D
4106041
Instruction Manual, BioLogic HR Valve Plumbing Diagrams, V7-3, AV7-3, Rev A
4106050
Instruction Manual, BioLogic™ HR Sample Inject Port Installation, V7-3, AV7-3 Valves, Rev B
4106054
Instruction Manual, BioLogic™ HR Gradient Pump Maintenance Kit, Rev C
4106248
Instruction Manual, BioLogic DuoFlow Chromatography System Software Version 5.0, Rev A
4006210
Instruction Manual, BioLogic™ Chromatography System
4106171
Instruction Manual, BioLogic DuoFlow Software Version 3.0 Discrepancies Solutions, Rev A
4106181
Instruction Manual, BioLogic DuoFlow Chromatography System Version 4.0 Software New Features, Rev A
4006208
Instruction Manual, BioLogic DuoFlow™ Chromatography System Starter Kit, Rev B
4106249
Instruction Manual, BioLogic DuoFlow Chromatography System 5.0 Software New Features, Rev A
4106039
Instruction Manual, BioLogic Column Rack Expansion Kit, Rev A
4106525
Instruction Manual Insert, BioLogic pH Monitor, Rev B
4106523
Instruction Manual Insert, BioLogic DuoFlow System USB Bitbus Communicator, Rev B
4106520
Instruction Manual Insert, BioLogic™ DuoFlow F10 Tubing Kit, Rev C
4006229
Instruction Manual, BioLogic DuoFlow™ Chromatography System, Rev B