(Discontinued) BioLogic DuoFlow 40 System

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Overview

The BioLogic DuoFlow family of chromatography systems offers flexibility with multiple system configurations, many optional upgrades, and a common software platform that is intuitive and easy to follow.
Read full description Ordering Information

Description

Description

The BioLogic DuoFlow family of chromatography systems offers flexibility with multiple system configurations, many optional upgrades, and a common software platform that is intuitive and easy to follow. These systems can be used on the laboratory bench or in a coldroom.

The BioLogic DuoFlow 40 system includes an F40 workstation for a flow rate of up to 40 ml/min* at 1,000 psi (66 bar, 6.6 MPa). A Model MX-1 mixer, 3-tray rack, AVR7-3 sample inject valve, fittings kit, UV detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, UNO Q1 column, and instructions are also included.

A Dell PC controller enables easy communication with the workstation and peripheral devices via an external USB Bitbus communicator. The controller includes the Windows XP operating system, application software, keyboard, mouse, and high-resolution flat-panel monitor.

BioLogic DuoFlow modular components allow the system to meet both laboratory space and application requirements; as requirements change, systems may be easily reconfigured and seamlessly upgraded with increased functionality, such as higher flow rates, sophisticated detection capabilities, pH monitoring, column scouting, and buffer blending.

* To double the flow rate to up to 80 ml/min, use the BioLogic Maximizer valve system. The optional F10 pump kit enables a flow rate of 0.01–10 ml/min at 3,500 psi (233 bar, 23 MPa).

Specifications

Specifications

Specifications
BioLogic DuoFlow Systems
Operating Limits
 
Flow rate
40 ml/min
Pressure
1,000 psi (6 6 MPa) at 40 ml/min
Detection
 
UV Detection
Standard 254 and 280 nm filters; optional 214,
UV Detection
analytical flow cell; optional 2 mm preparative
Conductivity
Standard
pH
Standard (BioLogic DuoFlow Maximizer)
Other
Optional signal import module (SIM) adds pH, refractive index, and fluorescence detectors
Sample Loading
 
Large samples/
Optional SV5-4 valve, Model EP-1 Econo
buffer selection
pump or Econo gradient pump
Single sample
AVR7-3 valve
Multiple samples
Optional DynaLoop sample loops (25 ml)
Fraction Collection
 
General
BioFrac or Model 2110 fraction collector
Large-volume
BioFrac fraction collector with optional
Small-volume
BioFrac or Model 2110 fraction collector
Special Applications
 
Buffer blending
BioLogic Maximizer valve system (BioLogic DuoFlow Maximizer, BioLogic DuoFlow Pathfinder systems); optional in BioLogic DuoFlow, BioLogic QuadTec systems
Column scouting/
Optional AVR9-8 valves for .8 columns
column switching
or additional AVR7-3 valve for 2 columns
Reverse flow
Additional AVR7-3 valve
Solvent Compatibility
All commonly used chromatographic solvents
Operating temperature
4–40°C
Dimensions (W x D x H)
35 x 41 x 81 cm (13.8 x 16.1 x 31.9")
Weight
70 kg (154 lb)

Ordering

Ordering

items

Use the filters below to refine results!

760-4037_tn.jpg
7604037
100/120 V, medium-pressure chromatography system, includes Dell controller and monitor, F40 workstation, mixer, rack, AVR7-3 valve, UV detector with flow cell and filters, conductivity monitor, starter kit
760-4037_tn.jpg
7604036
100/120 V, medium-pressure chromatography system, includes Dell controller, F40 workstation, MX-1 mixer, 3-tray rack, AVR7-3 valve, UV detector with flow cell and filters, starter kit, Japan and Korea only
760-4047_tn.jpg
7604047
100/120 V, medium-pressure chromatography system, includes fraction collector, Dell controller and monitor, F40 workstation, AVR7-3 valve, UV detector with flow cell and filters, conductivity monitor
760-4047_tn.jpg
7604046
100/120 V, medium-pressure chromatography system, includes fraction collector, Dell controller, F40 workstation, mixer, rack, AVR7-3 valve, UV detector with flow cell and filters, starter kit, Japan and Korea only
760-4047_tn.jpg
7604048
220/240 V, medium-pressure chromatography system, includes fraction collector, Dell controller, F40 workstation, AVR7-3 valve, UV detector with flow cell and filters, conductivity monitor, starter kit

Accessories

Accessories

760-0184_tn.jpg
7600184
Kit includes 2 piston seals, 4 check valves, seal removal tool, 2 O-rings, to service one F40 pump
760-0172_tn.jpg
(Discontinued) F40 Piston Seals
7600172
Pkg of 2, piston seals, and seal tool, to service one F40 pump
760-0173_tn.jpg
(Discontinued) F40 Piston Kit
7600173
Kit, includes 2 pistons, to service one F40 pump
750-0162_tn.jpg
(Discontinued) Check Valve
7500162
Pkg of 1, check valve, for use with BioLogic DuoFlow workstation, (4 check valves required per pump)
750-0703_tn.jpg
(Discontinued) Inline Filter Kit
7500703
Filter kit, includes 1 inline filter and 2 replacement frits, for use with BioLogic DuoFlow™ and NGC™ Systems
760-0110_tn.jpg
(Discontinued) F10 Pump Kit
7600110
Kit to convert an F40 workstation to an F10, includes 2 assembled pumpheads with seals and check valves, 4 piston assemblies, F10 tubing kit, tools; enables flow rates as low as 0.01 ml/min
750-0230_tn.jpg
7500230
Pkg of 1, for use with BioLogic DuoFlow™ and NGC™ Workstations
760-0652_tn.jpg
(Discontinued) F40 Tubing Kit
7600652
High-pressure tubing kit, includes precut and fitted PTFE, Tefzel, and green PEEK tubing, for installation of BioLogic DuoFlow basic chromatography system running at flow rates >40 ml/min
750-0251_tn.jpg
(Discontinued) BioLogic Rack
7500251
Includes rack tray, 8 sleeves, 2 short vertical bars, 2 long vertical bars, column clamp set, 5 bar clamps, 4 cable organizers
750-0268_tn.jpg
7500268
Kit, includes 2 rack trays, 2 long vertical bars, 16 sleeves, for use with BioLogic Rack (7500251)
750-0262_tn.jpg
(Discontinued) Vertical Bars
7500262
Pkg of 2, 64 cm long bars, for use with BioLogic Rack (7500251)
750-0263_tn.jpg
(Discontinued) Vertical Bars
7500263
Pkg of 2, 10 cm short bars, for use with BioLogic Rack (7500251)
horizontal-bar-kit-750-0264-thumb.jpg
(Discontinued) Horizontal Bar Kit
7500264
Bar kit, includes 2 tie bars, 4 bar clamps, for use with BioLogic Rack (7500251)
750-0260_tn.jpg
(Discontinued) Column Clamp Set
7500260
Column clamp set, includes 1 column clamp assembly, for use with BioLogic Rack (7500251)
750-0265_tn.jpg
(Discontinued) Bar Clamps
7500265
Pkg of 5, bar clamps, for use with BioLogic Rack (7500251)
760-0406_tn.jpg
7600406
Pkg of 1, high-pressure sample inject valve, 9-port, 8-position, 3,500 psi (233 bar) limit, for use with BioLogic DuoFlow system
760-0408_tn.jpg
7600408
Pkg of 1, high-pressure valve, 9-port, 8-position, 3,500 psi (233 bar) limit, for use with BioLogic DuoFlow system
750-0415_tn.jpg
(Discontinued) SV5-4 Select Valve
7500415
Pkg of 1, 5-port, 4-position solenoid valve, 30 psi (2 bar) limit
760-0410_tn.jpg
7600410
Pkg of 1, solenoid valve, 3-port, 2-position, 30 psi (2 bar) limit, for use with BioLogic DuoFlow system
750-0471_tn.jpg
7500471
Pkg of 1, sample injection port, for use with AVR7-3 automated sample inject valve (#760-0406)
125-0224_tn.jpg
(Discontinued) Injection Needle
1250224
Pkg of 1, 22-gauge, injection needle, blunt, for use with BioLogic DuoFlow system
750-0490_tn.jpg
7500490
Pkg of 1, sample loop kit, includes 100, 250, and 500 μl PEEK loops
750-0491_tn.jpg
7500491
Pkg of 1, sample loop kit, includes 1, 2, and 5 ml PEEK loops

Kits & Reagents

Kits & Reagents

760-0550_tn.jpg
7600550
Fittings kit contains PEEK and Tefzel nuts, ferrules, unions, plugs, and luer syringe, for use with BioLogic DuoFlow and NGC systems
7600135
Kit includes protein anion exchange standards, 50 µL injection loop, buffers, 1 mL syringe, and manual
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(Discontinued) UNO Q1 Column
7200001
Pkg of 1, 7 x 35 mm, 1.3 ml, prepacked monolith ion exchange column, 20 mg max. protein load, 0.5–5.0 ml/min flow rate, operating pressure 700 psi
720-0003_tn.jpg
(Discontinued) UNO Q6 Column
7200003
Pkg of 1, 12 x 53 mm, 6 ml, prepacked monolith ion exchange column, 90 mg max. protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
720-0005_tn.jpg
(Discontinued) UNO Q12 Column
7200005
Pkg of 1, 15 x 68 mm, 12 ml, prepacked monolith ion exchange column, 180 mg max. protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
720-0021_tn.jpg
(Discontinued) UNO S1 Column
7200021
Pkg of 1, 7 x 35 mm, 1.3 ml, prepacked monolith ion exchange column, 20 mg max. protein load, 0.5–5.0 ml/min flow rate, operating pressure 700 psi
720-0023_tn.jpg
(Discontinued) UNO S6 Column
7200023
Pkg of 1, 12 x 53 mm, 6 ml, prepacked monolith ion exchange column, 90 mg max protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
720-0025_tn.jpg
(Discontinued) UNO S12 Column
7200025
Pkg of 1, 15 x 68 mm, 12 ml, prepacked monolith ion exchange column, 180 mg max. protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
732-4650_tn.jpg
7324650
Includes one 1 ml cartridge each of UNOsphere™ S, UNOsphere Q, Macro-Prep® High Q, Macro-Prep High S, Macro-Prep DEAE
732-4651_tn.jpg
7324651
Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue

Documents

Documents

Number Description Options
6439
Improving Aggregrate Removal from a Monoclonal Antibody Feedstream Using High-Resolution Cation Exchange Chromatography, Rev B
DC7602030
Declaration of Conformity, BioLogic USB Bitbus Device, Rev A
2780
Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A
2849
Protein A Removal From IgG on CHT Ceramic Hydroxyapatite Support, Rev A
5342
Automated Three-Dimensional Purification Method for Histidine-Tagged Proteins Using the BioLogic DuoFlow Maximizer Chromatography System, Rev A
5334
A Method for Rapid, Large-Scale Removal of Albumin and IgG From Human Serum Using the BioLogic DuoFlow Chromatography System, Rev A
5251
Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins, Rev A
3039
Separation Reproducibility With the BioLogic Chromatography Systems
5326
Chromatography Column Connection Guide, Rev A
5369
Biologic DuoFlow System Family Brochure, Rev A
2584
BioLogic DuoFlow™ Valving Guide, Rev E
2815
BioLogic DuoFlow™ Valves Brochure, Rev C
2687
BioLogic DuoFlow Chromatography System Brochure, Rev D
4106041
Instruction Manual, BioLogic HR Valve Plumbing Diagrams, V7-3, AV7-3, Rev A
4106050
Instruction Manual, BioLogic™ HR Sample Inject Port Installation, V7-3, AV7-3 Valves, Rev B
4106054
Instruction Manual, BioLogic™ HR Gradient Pump Maintenance Kit, Rev C
4106248
Instruction Manual, BioLogic DuoFlow Chromatography System Software Version 5.0, Rev A
4006210
Instruction Manual, BioLogic™ Chromatography System
4106171
Instruction Manual, BioLogic DuoFlow Software Version 3.0 Discrepancies Solutions, Rev A
4106181
Instruction Manual, BioLogic DuoFlow Chromatography System Version 4.0 Software New Features, Rev A
4006208
Instruction Manual, BioLogic DuoFlow™ Chromatography System Starter Kit, Rev B
4106249
Instruction Manual, BioLogic DuoFlow Chromatography System 5.0 Software New Features, Rev A
4106039
Instruction Manual, BioLogic Column Rack Expansion Kit, Rev A
4106525
Instruction Manual Insert, BioLogic pH Monitor, Rev B
4106523
Instruction Manual Insert, BioLogic DuoFlow System USB Bitbus Communicator, Rev B
4106520
Instruction Manual Insert, BioLogic™ DuoFlow F10 Tubing Kit, Rev C
4006229
Instruction Manual, BioLogic DuoFlow™ Chromatography System, Rev B
2078
Purification of Marine Algal Vanadate Bromoperoxidase Dodecamer by Preparative Native PAGE on a BioLogic-Driven Mini Prep Cell, Rev B