Criterion Stain Free™ Tris-HCl Gels

Image
lsr_criterion_stainfree_Tris_HCI_gels.jpg

Description

Description

Discontinued Product

Criterion Stain-Free Tris-HCl Gels have been replaced with Criterion™ TGX Stain-Free™ Gels

To find a recommended replacement gel for a particular discontinued Tris-HCl gel, either:

If no replacement gel is listed, please contact Technical Support for questions about transitioning to a new product.

 

What Is Stain-Free
Technology?

Find out more »

Bio-Rad Stain-Free Gel Imaging Systems allow direct visualization, analysis, and documentation of protein samples in SDS-PAGE gels with the single touch of a button, without staining, destaining, or gel drying procedures. The complete system is composed of the Gel Doc™ EZ imager*, Criterion Stain-Free gels, and Image Lab™ Software. It is a dedicated,

small-format, easy-to-use system that uses standard conventions of SDS-PAGE. This stain-free system is ideal for quick sample assessment prior to purification procedures and does not require any changes to the sample preparation process. The system is also useful as a precursor to blotting and profiling workflows in which Coomassie Blue staining is ordinarily used as a detection method for purity assessment, quantitation, protein characterization (western blotting), and profiling (MALDI-TOF, LC-MS/MS). Benefits include:

 

  • Fast results — elimination of staining, destaining, and gel drying steps shortens the electrophoresis portion of the experimental workflow, such as sample assessment and data analysis in protein purification; researchers can plan for the next purification step or for the next project
  • Reproducibility — gel imaging and analysis is automated, enabling reproducible results. There is no background variability within a gel or between gels (as is often seen with standard Coomassie Blue staining); Stain-Free Imaging enables standardization of methods
  • Green technology — acetic acid and methanol are eliminated, reducing organic waste
  • Ease of use — one-touch gel image analysis processing and data reporting is accomplished through a single user interface, reducing the number of steps to achieve results

Criterion Stain-Free Gels

Criterion Stain-Free gels are formulated with a protein visualization compound and Tris-HCl for PAGE applications. The most common protein electrophoretic application, SDS-PAGE, separates complex protein mixtures by size, allowing estimation of the molecular weight of sample proteins. The gels are made without SDS. By omitting the SDS from the running buffer, the gels can be used to run proteins under nondenaturing conditions for subsequent analysis of their native conformation and activity. Both single percentage and linear gradient gels are available.

Gel Doc EZ Imager*

The Gel Doc EZ system is a compact and automated imaging system. Trays specific for different applications afford flexibility and the Image Lab software provides publication-quality images and analyzed results with a push of a button.

*The Gel Doc EZ imager has replaced the discontinued Criterion Stain-Free Imaging System.

Specifications

Specifications

Gel dimensions (W x L)
13.3 x 8.7 cm
Gel cassette dimensions (W x L)
15 x 10.6 cm
Gel storage conditions
4°C
Gel shelf life*
12 weeks
* From date of manufacture.
 

Ordering

Ordering

items

Use the filters below to refine results!

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3450412
Discontinued item
Replaced with 4-20% Criterion TGX Stain-Free Protein Gel, 12+2 well, 45 µl ()
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3450418
Discontinued item
Replaced with 4-20% Criterion TGX Stain-Free Protein Gel, 18-well, 30 µl ()
345-0426_tn.jpg
3450426
Discontinued item
Replaced with 4-20% Criterion TGX Stain-Free Protein Gel, 26-well, 15 µl ()
345-1012_tn.jpg
3451012
Discontinued item
Replaced with 10% Criterion TGX Stain-Free Protein Gel, 12+2 well, 45 µl ()
345-1018_tn.jpg
3451018
Discontinued item
Replaced with 10% Criterion TGX Stain-Free Protein Gel, 18-well, 30 µl ()
345-8161_tn.jpg
3458161
Discontinued item
Replaced with 8-16% Criterion TGX Stain-Free Protein Gel, 11 cm IPG/prep+1 well, 550 µl ()
345-0412_tn.jpg
3458162
Discontinued item
Replaced with 8–16% Criterion™ TGX Stain-Free™ Protein Gel, 12+2 well, 45 µl ()
345-0426_tn.jpg
3458166
Discontinued item
Replaced with 8–16% Criterion; TGX Stain-Free Protein Gel, 26-well, 15 µl (5678105)

Accessories

Accessories

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4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes

Kits & Reagents

Kits & Reagents

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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610734
Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
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1610744
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610771
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
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(Discontinued) 10x Tris/Glycine/SDS
1610772
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610737
30 ml, premixed protein sample buffer for SDS-PAGE
161-0738_tn.jpg
1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
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(Discontinued) Dithiothreitol (DTT)
1610610
1 g, dithiothreitol (DTT) reducing agent
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(Discontinued) Dithiothreitol (DTT)
1610611
5 g, dithiothreitol (DTT) reducing agent
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(Discontinued) 2-Mercaptoethanol
1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)

Documents

Documents

Number Description Options
6040
Electrophoresis Guide, Interactive PDF, Rev C
5793
Criterion Stain-Free Gel Imaging System Brochure
10040758
Instruction Manual, Image Lab™ Software: Imaging Stain-Free Gels and Blots, Rev B
5782
In-Gel Protein Quantitation Using the Criterion Stain-Free Gel Imaging System
5939
Overcoming the Coomassie Blues, Rev A
5974
Mini-PROTEAN® and Criterion™ TGX Stain-Free™ Precast Gels Product Information Sheet, Rev E
6001
Rapid Validation of Purified Proteins Using Criterion Stain-Free Gels
10014472
Instruction Manual, Criterion Stain-Free Imaging System
4110001
Instruction Manual, Criterion™ Gel Application Guide, Ver H
6453
Utilize Stain-Free Technology in a Variety of Workflows, Rev B
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6507
TGX and TGX Stain-Free Gels: Publications List