Criterion Stain Free™ Tris-HCl Gels

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Criterion Stain Free Tris-HCl Gels

Overview

Criterion™ Tris-HCI Gels
Criterion Cells, Blotter, and Accessories

Criterion family of products: from left to right, Criterion Cell, Criterion precast gel, Criterion blotter, assembly tray with gel holder cassette, roller, fiber pads and blotting filter paper, Criterion Dodeca Cell.

Discontinued Product
Criterion Stain Free Tris-HCl Gels have been replaced with Criterion™ TGX Stain-Free™ Gels.
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Description

Discontinued Product

Criterion Stain-Free Tris-HCl Gels have been replaced with Criterion™ TGX Stain-Free™ Gels

To find a recommended replacement gel for a particular discontinued Tris-HCl gel, either:

If no replacement gel is listed, please contact Technical Support for questions about transitioning to a new product.

 

What Is Stain-Free
Technology?

Find out more »

Bio-Rad Stain-Free Gel Imaging Systems allow direct visualization, analysis, and documentation of protein samples in SDS-PAGE gels with the single touch of a button, without staining, destaining, or gel drying procedures. The complete system is composed of the Gel Doc™ EZ imager*, Criterion Stain-Free gels, and Image Lab™ Software. It is a dedicated,

small-format, easy-to-use system that uses standard conventions of SDS-PAGE. This stain-free system is ideal for quick sample assessment prior to purification procedures and does not require any changes to the sample preparation process. The system is also useful as a precursor to blotting and profiling workflows in which Coomassie Blue staining is ordinarily used as a detection method for purity assessment, quantitation, protein characterization (western blotting), and profiling (MALDI-TOF, LC-MS/MS). Benefits include:

 

  • Fast results — elimination of staining, destaining, and gel drying steps shortens the electrophoresis portion of the experimental workflow, such as sample assessment and data analysis in protein purification; researchers can plan for the next purification step or for the next project
  • Reproducibility — gel imaging and analysis is automated, enabling reproducible results. There is no background variability within a gel or between gels (as is often seen with standard Coomassie Blue staining); Stain-Free Imaging enables standardization of methods
  • Green technology — acetic acid and methanol are eliminated, reducing organic waste
  • Ease of use — one-touch gel image analysis processing and data reporting is accomplished through a single user interface, reducing the number of steps to achieve results

Criterion Stain-Free Gels

Criterion Stain-Free gels are formulated with a protein visualization compound and Tris-HCl for PAGE applications. The most common protein electrophoretic application, SDS-PAGE, separates complex protein mixtures by size, allowing estimation of the molecular weight of sample proteins. The gels are made without SDS. By omitting the SDS from the running buffer, the gels can be used to run proteins under nondenaturing conditions for subsequent analysis of their native conformation and activity. Both single percentage and linear gradient gels are available.

Gel Doc EZ Imager*

The Gel Doc EZ system is a compact and automated imaging system. Trays specific for different applications afford flexibility and the Image Lab software provides publication-quality images and analyzed results with a push of a button.

*The Gel Doc EZ imager has replaced the discontinued Criterion Stain-Free Imaging System.


Specifications

Gel dimensions (W x L) 13.3 x 8.7 cm
Gel cassette dimensions (W x L) 15 x 10.6 cm
Gel storage conditions 4°C
Gel shelf life* 12 weeks
* From date of manufacture.  

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4–20% Criterion Stain-Free Tris-HCl Protein Gel, 12+2 well, 45 µl

3450412
Discontinued item
Replaced with 4-20% Criterion TGX Stain-Free Protein Gel, 12+2 well, 45 µl ()

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) 4–20% Criterion Stain-Free Tris-HCl Protein Gel, 18 well, 30 µl

3450418
Discontinued item
Replaced with 4-20% Criterion TGX Stain-Free Protein Gel, 18-well, 30 µl ()

This product is no longer available. Please contact technical support for more information on replacement product.
4–20% Criterion Stain-Free Tris-HCl Protein Gel, 26 well, 15 µl

3450426
Discontinued item
Replaced with 4-20% Criterion TGX Stain-Free Protein Gel, 26-well, 15 µl ()

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) 10% Criterion Stain-Free Tris-HCl Protein Gel, 12+2 well, 45 µl

3451012
Discontinued item
Replaced with 10% Criterion TGX Stain-Free Protein Gel, 12+2 well, 45 µl ()

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) 10% Criterion Stain Free™ Tris-HCl Protein Gel, 18 well, 30 µl

3451018
Discontinued item
Replaced with 10% Criterion TGX Stain-Free Protein Gel, 18-well, 30 µl ()

This product is no longer available. Please contact technical support for more information on replacement product.
8–16% Criterion Stain-Free Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3458161
Discontinued item
Replaced with 8-16% Criterion TGX Stain-Free Protein Gel, 11 cm IPG/prep+1 well, 550 µl ()

This product is being discontinued soon. Please contact customer service for more information.
8–16% Criterion Stain-Free Tris-HCl Protein Gel, 12+2 well, 45 µl

3458162
Discontinued item
Replaced with 8–16% Criterion™ TGX Stain-Free™ Protein Gel, 12+2 well, 45 µl ()

This product is being discontinued soon. Please contact customer service for more information.
8–16% Criterion-Stain Free Tris-HCl Protein Gel, 26 well, 15 µl

3458166
Discontinued item
Replaced with 8–16% Criterion; TGX Stain-Free Protein Gel, 26-well, 15 µl (5678105)

This product is being discontinued soon. Please contact customer service for more information.

Accessories

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list price. If the problem persists, please call customer service at or send an
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Mini-PROTEAN® Cassette Opening Lever

4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes

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$5.00 
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Kits & Reagents

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10x Tris/Glycine/SDS

1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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$32.00 
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10x Tris/Glycine Buffer for Western Blots and Native Gels

1610734
Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

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$32.00 
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10x Tris/Tricine/SDS Running Buffer, 1 L

1610744
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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$100.00 
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10x Tris/Glycine Buffer for Western Blots and Native Gels

1610771
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

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$108.00 
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10x Tris/Glycine/SDS

1610772
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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$108.00 
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2x Laemmli Sample Buffer

1610737
30 ml, premixed protein sample buffer for SDS-PAGE

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$14.00 
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Native Sample Buffer for Protein Gels, 30 ml

1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue

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$16.00 
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Dithiothreitol (DTT)

1610610
1 g, dithiothreitol (DTT) reducing agent

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$31.00 
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Dithiothreitol (DTT)

1610611
5 g, dithiothreitol (DTT) reducing agent

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$94.00 
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2-Mercaptoethanol

1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)

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$27.00 
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Documents

Number Description Options
6040 Electrophoresis Guide, Interactive PDF, Rev C Click to download
5793 Criterion Stain-Free Gel Imaging System Brochure Click to download
10040758 Instruction Manual, Image Lab™ Software: Imaging Stain-Free Gels and Blots, Rev B Click to download
5782 In-Gel Protein Quantitation Using the Criterion Stain-Free Gel Imaging System Click to download
5939 Overcoming the Coomassie Blues, Rev A Click to download
5974 Mini-PROTEAN® and Criterion™ TGX Stain-Free™ Precast Gels Product Information Sheet, Rev E Click to download
6001 Rapid Validation of Purified Proteins Using Criterion Stain-Free Gels Click to download
10014472 Instruction Manual, Criterion Stain-Free Imaging System Click to download
4110001 Instruction Manual, Criterion™ Gel Application Guide, Ver H Click to download
6453 Utilize Stain-Free Technology in a Variety of Workflows, Rev B Click to download [ Add to Cart (Free) ]
6507 TGX and TGX Stain-Free Gels: Publications List Click to download

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