Cloning and Sequencing Explorer Series

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Cloning and Sequencing Explorer Series

Overview

Cloning and Sequencing Explorer Series Flowchart
Cloning and Sequencing Explorer Series Flowchart
Cloning and Sequencing Explorer Series Contents
Lab contains sufficient materials for 12 workstations (2–4 students per workstation).
Nucleic Acid Extraction Module 1
GAPDH PCR Module 1
Small UView™ DNA Electrophoresis Reagents Module 1
PCR Kleen™ Spin Purification Module 1
Ligation and Transformation Module 1
Microbial Culturing Module 1
Aurum™ Plasmid Mini Purification Module 1
Sequencing and Bioinformatics Module 1
1.5 ml EZ Micro™ test tubes, pkg. of 500 1
Required Accessories Not Included in Kit:
Plant samples
95–100% lab grade ethanol
Horizontal gel electrophoresis chambers
Thermal cycler
Adjustable micropipets
Pipet tips, aerosol barrier
Incubation oven
Water bath and dry bath
Microcentrifuges capable of greater than 12,000 x g
Power supplies
Gel documentation system
Shaking water bath or shaking incubator
Recommended (Optional) Accessories:
Vortexer
Microwave oven/autoclave
Gel documentation system
Spectrophotometer and cuvettes
Available Separately:
Nucleic Acid Extraction Module (1665005EDU) 1
GAPDH PCR Module (1665010EDU) 1
Small UView DNA Electrophoresis Reagents Module (1660462EDU) 1
PCR Kleen Spin Purification Module (7326300EDU) 1
Ligation and Transformation Module (1665015EDU) 1
Microbial Culturing Module (1665020EDU) 1
Aurum Plasmid Mini Purification Module (7326400EDU) 1
Sequencing and Bioinformatics Module (1665025EDU) 1
1.5 ml EZ Micro™ test tubes, pkg. of 500 (2239480EDU) 1
Cloning and Sequencing Explorer Planning Guide

Overview

The intention of this planning guide is to provide an overview of the entire student research project including details of timelines, materials included, and equipment requirements so that instructors may prepare their curriculum and estimate budgetary requirements in advance. These are estimates based on current information available, and may change.


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The complete Cloning and Sequencing Explorer series is a 6–8 week series of laboratory activities in which students clone and analyze a portion of the GAPC gene. Activities range from DNA extraction to computer-based sequence analysis.


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  • Documents

Description

The Cloning and Sequencing Explorer Series gives your students the tools to perform novel research and contribute to the world's body of scientific knowledge. Over 8 modules, students use classic and cutting-edge molecular biology techniques to clone and sequence a portion of the GAPC gene from a plant of their choice. GAPC is a housekeeping gene in the glyeraldehyde-3-phosphate dehydrogenase (GAPDH) family and is well conserved among plants.

The Cloning and Sequencing Explorer Series guides students through the following experimental workflow steps:

  1. Extract genomic DNA from plants
  2. Amplify a GAPDH gene using PCR and analyze using electrophoresis
  3. Purify the PCR product
  4. Ligate the PCR product into a plasmid
  5. Transform the ligated plasmid into bacteria
  6. Grow minipreps of the transformed bacteria
  7. Isolate plasmid DNA and analyze by restriction digestion
  8. Sequence plasmid DNA (sequencing service not included)
  9. Analyze GAPDH gene sequence using bioinformatics

Features and Benefits

Designed by undergraduate and community college educators, this course was specifically developed to engage your students in authentic research, while ensuring success for both you and them. Tailored experimental controls allow your students to:

  • Continue the experiment to its conclusion even with negative results
  • Make mistakes, learn from them, and still complete every step of the workflow
  • Experience firsthand the satisfaction of completing a successful research project
  • Apply molecular biology skills and techniques to complete a real-world research project

The Cloning and Sequencing Explorer Series allows students to apply molecular biology skills and techniques to complete a real-world research project, potentially encouraging them to pursue a career in research.

Now with Geneious Prime!

geneiousThe Cloning and Sequencing Explorer Series now includes a 4-month subscription to Geneious Prime Bioinformatics Software to support your students' scientific discovery.

For more information, visit the Sequencing and Bioinformatics Module product page.

More Information

  • 6–8 week series of laboratory activities made up of eight modular kits
  • Provides sufficient materials for 12 student workstations, up to four students per workstation
  • Sequencing service not included.

Additional Resources


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Cloning and Sequencing Explorer Series

1665000EDU
Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum (sequencing service not included); education use only

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$1,995.00 
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$1,525.18  
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Refills

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list price. If the problem persists, please call customer service at or send an
email to

Nucleic Acid Extraction Module

1665005EDU
First module in Cloning and Sequencing Explorer Series classroom study kit, includes buffers, DTT, test tubes

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$215.00 
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$172.00  
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GAPDH PCR Module

1665010EDU
Second module in Cloning and Sequencing Explorer Series classroom study kit, includes primers, PCR master mix, test tubes/holders, curriculum

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$498.75 
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$399.00  
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Small Ethidium Bromide DNA Electrophoresis Reagents Pack

1660451EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, ethidium bromide, buffer; education use only

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$143.00 
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$53.00  
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Small Fast Blast DNA Electrophoresis Reagents Pack

1660450EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only

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$114.00 
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$55.01  
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Medium Fast Blast DNA Electrophoresis Reagent Pack

1660455EDU
Agarose gel preparation kit,makes 90–270 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only

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$311.00 
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$199.01  
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Large Fast Blast DNA Electrophoresis Reagent Pack

1660460EDU
Agarose gel preparation kit,makes 360–1,080 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only

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$940.00 
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$588.06  
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Medium Ethidium Bromide DNA Electrophoresis Reagents Pack

1660456EDU
Agarose gel preparation kit,makes 90–270 mini gels, includes agarose powder, ethidium bromide, buffer; education use only

List Price: 
 
$322.00 
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$192.01  
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Large Ethidium Bromide DNA Electrophoresis Reagents Pack

1660461EDU
Agarose gel preparation kit,makes 360–1,080 mini gels, includes agarose powder, ethidium bromide, buffer; education use only

List Price: 
 
$923.00 
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$560.08  
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PCR Kleen™ Spin Purification Module

7326300EDU
Fourth module in Cloning and Sequencing Explorer Series classroom study kit, includes PCR Kleen Spin columns, test tubes, curriculum, for 12 workstations; education use only

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$105.00 
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$80.00  
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Ligation And Transformation Module

1665015EDU
Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only

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$406.25 
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$325.00  
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Microbial Culturing Module

1665020EDU
Sixth module in Cloning and Sequencing Explorer Series classroom study kit, includes ampicillin, petri dishes, E. coli strain HB101 K-12, curriculum, and more, for 12 workstations; education use only

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$121.25 
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$97.00  
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Aurum Plasmid Mini Purification Kit

7326400EDU
Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps

List Price: 
 
$217.14 
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$152.00  
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Sequencing and Bioinformatics Module

1665025EDU
Eighth module in Cloning and Sequencing Explorer Series classroom study kit, includes primers, plasmid, barcoded 96-well plate, test tubes, curriculum, and more, for 12 workstations; education use only (Sequencing service not included)

List Price: 
 
$327.50 
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$262.00  
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(Discontinued) Cloning and Sequencing Explorer Series Instruction Manual

1665001EDU
CD-ROM for use with Cloning and Sequencing Explorer series (1665000EDU), includes electronic version of instruction manual (PDF format); education use only

This product is no longer available. Please contact technical support for more information on replacement product.

Documents

Number Description Options
10000069187 Bio-Rad Explorer Cloning and Sequencing Explorer Series, Ver G Click to download
5773 Cloning and Sequencing Explorer Series Planning Guide, Bio-Rad Explorer™ , Ver C Click to download
6672 Map of AP-Aligned Bio-Rad Kits with Essential Knowledge Click to download
6673 Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories Click to download
6602 UView™ 6x Loading Dye and Stain Guide, Bio-Rad Explorer™ Click to download

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