CHT Ceramic Hydroxyapatite Type I Support

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Overview

CHT ceramic hydroxyapatite, (Ca5(PO4)3OH)2 , support is a leading purification medium for today's demanding downstream process industry.
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Description

Description

Free Ceramic Hydroxyapatite reference wall charts for process purification

FREE Ceramic hydroxyapatite reference wall charts

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Hydroxyapatite (Ca5(PO4)3OH)2 is a form of calcium phosphate used in the chromatographic separation of biomolecules. Sets of five calcium doublets (C-sites) and pairs of hydroxyl-containing phosphate triplets (P-sites) are arranged in a repeating geometric pattern. Repeating hexagonal structures can be seen in electron micrographs of the material; space-filling models and repeat structure from Raman spectroscopy have also been constructed.

CHT ceramic hydroxyapatite support is a leading purification medium for today's demanding downstream process industry. Its mixed-mode support offers unique selectivities and often separates biomolecules that appear homogeneous using other chromatographic methods. The diverse binding capabilities of CHT for host cell proteins, leached protein A, antibody dimmers and aggregates, nucleic acids, and viruses allow its use at any stage from initial capture to final polishing.

The robust properties of CHT ceramic hydroxyapatite improve efficiency, yield, and financial value through:

  • Excellent capture at elevated flow rates, enabling processing at all scales
  • Large capacity for high-titer upstream feedstocks
  • Exceptional selectivity, allowing a two-step chromatographic process

Two types of CHT ceramic hydroxyapatite, Type I and Type II, are available in three particle sizes, 20, 40, and 80 µm. Both types have elution characteristics similar to crystalline hydroxyapatite but also have some important differences:

  • Type I, sintered at 400°C, has a higher protein binding capacity and better capacity for acidic proteins
  • Type II, sintered at 700°C, has a lower protein binding capacity, but offers better resolution of nucleic acids and certain proteins; it also has a very low affinity for albumin and is particularly well-suited for the purification of many species and classes of immunoglobulins

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO9001 registered corporation.

Ordering

Ordering

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1582000
10 g, CHT ceramic hydroxyapatite multimodal chromatography media, Type I, 20 μm particle size
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1570020
100 g, CHT ceramic hydroxyapatite multimodal chromatography media, Type 1, 20 μm particle size
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157-0021
1 kg, CHT ceramic hydroxyapatite multimodal chromatography media, Type I, 20 μm particle size
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157-0025
5 kg, CHT ceramic hydroxyapatite multimodal chromatography media, Type I, 20 μm particle size
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1584000
10 g, CHT ceramic hydroxyapatite multimodal chromatography media, Type I, 40 μm particle size
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1570040
100 g, CHT ceramic hydroxyapatite multimodal chromatography media, Type I, 40 μm particle size
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157-0041
1 kg, CHT ceramic hydroxyapatite multimodal chromatography media, Type I, 40 μm particle size
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157-0045
5 kg, CHT ceramic hydroxyapatite multimodal chromatography media, Type I, 40 μm particle size
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1588000
10 g, CHT ceramic hydroxyapatite multimodal chromatography media, Type I, 80 μm particle size
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1570080
100 g, CHT ceramic hydroxyapatite multimodal chromatography media, Type 1, 80 μm particle size
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157-0081
1 kg, CHT ceramic hydroxyapatite multimodal chromatography media, Type I, 80 μm particle size
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157-0085
5 kg, CHT ceramic hydroxyapatite multimodal chromatography media, Type I, 80 μm particle size

Documents

Documents

Number Description Options
7100
Maximize Monomer Recovery during mAb Purification, Ver A
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5667
CHT Ceramic Hydroxyapatite Product Information Sheet, Rev B
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6086
Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up
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5709
How CHT Ceramic Hydroxyapatite Works, Rev A
[ Add to Cart (Free) ]
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
6982
Mixed-Mode Resins/Media Selection Guide
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6983
Native and Recombinant Protein Purification Resins/Media Selection Card
6786
Monoclonal Antibody Purification Resin Selection Card, Ver A
6789
IgG Polyclonal Antibody Purification Resin Selection Card, Ver A
6791
Virus Purification Resin Selection Card, Ver A
6792
Aggregate Purification Resin Selection Card, Ver A
6955
Non–Affinity Based Purification Platform for Neutral/Basic IgMs (Poster)
6749
Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes, Ver A
[ Add to Cart (Free) ]
6549
Mammalian Virus Purification Using Ceramic Hydroxyapatite, Rev A
[ Add to Cart (Free) ]
2940
Removal of Aggregate from an IgG4 Product Using CHT™ Ceramic Hydroxyapatite Resin, Rev B
5739
Packing and Slurry-in-Place Procedures for CHT Ceramic Hydroxyapatite Using a Bio-Rad InPlace Process-Scale Chromatography Column, Rev A
[ Add to Cart (Free) ]
2731
Plasmid Purification Using CHT Ceramic Hydroxyapatite Support, Rev A
2156
CHT Ceramic Hydroxyapatite — A New Dimension in Chromatography of Biological Molecules, Rev C
2780
Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A
2524
Purification of Horse IgG T Using Macro-Prep DEAE and CHT Ceramic Hydroxyapatite Type I Supports, Rev C
2774
Purification of Transgenic Antibody From Corn Seed Using UNOsphere S and CHT Ceramic Hydroxyapatite Supports, Rev A
1986
Effect of pH on Gradient Elution of Proteins on Two Types of CHT Ceramic Hydroxyapatite, Rev B
1985
Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B.
2849
Protein A Removal From IgG on CHT Ceramic Hydroxyapatite Support, Rev A
2535
Purification of Recombinant Human alpha 1-Antitrypsin From Transgenic Milk Using CHT Ceramic Hydroxyapatite, Rev B
2575
Purification of a Calcium-Binding Protein Using CHT Ceramic Hydroxyapatite
5662
Process Chromatography Applications Laboratory Brochure, Rev B
2946
Plasmid DNA Adsorption Behavior on CHT Ceramic Hydroxyapatite Type II Support, Rev A
6278
High-Throughput Calcium Analysis of Hydroxyapatite Column Effluent, Rev A
2026
Evaluation of Different Approaches for the Chromatographic Purification of Monoclonal Antibodies
5853
Chimeric IgG Monoclonal Antibody Purification: A Comparative Study Using CHT Ceramic Hydroxyapatite and CFT Ceramic Fluoroapatite Chromatographic Media, Rev A
6870
Ceramic Apatite-Based Chromatography Media Publications List, Ver A
6946
Monoclonal Antibody Purification: Polish Purification Resins Brochure, Ver A
6886
Quick Monoclonal Antibody Purification Strategy: Affinity-Based Capture Flier, Ver A
6902
How Does CHT™ Ceramic Hydroxyapatite Media Work (Poster), Ver A
6932
Good Practices for CHT™ Ceramic Hydroxyapatite Media Usage (Poster), Ver A
6965
Recommendations for CHT™ Ceramic Hydroxyapatite Column Packing (Poster)
6966
Development of a Non–Affinity Based Purification Platform for Neutral/Basic IgMs