ChemiDoc MP Imaging System



Use the ChemiDoc MP Imaging System for imaging of gels and blots. Detect multiplex fluorescence, chemiluminescence, and a wide range of different stains.

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Stain Free Enabled

What is Stain-Free Technology?

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The ChemiDoc MP Imaging System is a full-feature instrument for imaging and analyzing gels and western blots. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs.

Complete Capabilities: Superior Images

Total Protein Normalization


Learn how to properly design a quantitative western blotting experiment and faithfully compare protein expression levels, without concern for changing or overexpressed HKP’s.
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Replaces Film


Save money on consumables and reduce environmental waste without compromising on performance. Get the sensitivity of film and the convenience of digital documentation.
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Multiplex Fluorescence
3 Colors


Save sample, avoid the errors commonly associated with stripping, reprobing, and cutting blots and answer more complex biological questions by optimizing your experiments to fluorescence.
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Features and Benefits

  • All-in-one flexible imaging — get precise, reproducible fluorescence, chemiluminescence, and colorimetric gel and blot detection, analysis and documentation in a single system
  • Improve your imaging capabilities with the ChemiDoc MP — benefit from the advantages of using a wider range of fluorescent reporters (RGB, far red, and near infrared) including StarBright Fluorophores; see the fluorophore compatibility chart
  • Multiplex fluorescent western blotting — detect up to three proteins simultaneously and eliminate the need for stripping and reprobing
  • Ease of use — features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images. Learn in minutes with on screen help
  • Stain-free protein normalization — stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminates the need for housekeeping proteins
  • Sample Trays and Smart Tray Technology — three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
  • Installation/Operational Qualification kit available for ensuring installation and calibration is properly executed for highly regulated laboratory environments.

Performing Stain-Free western blotting using the ChemiDoc MP System provides the ability to rapidly assess electrophoresis and blot transfer quality prior to blot processing, to perform total protein normalization and multiplexing using a wide rance of fluorophores.

Applications and Uses

  • Electrophoresis — protein, including stain-free, and nucleic acid gel imaging and documentation
  • Blotting — identify and quantify proteins, nucleic acids, protein-ligand interactions, etc. with fluorescence, chemiluminescence, or colorimetric detection

Image Lab Touch Software Demo

Explore how intuitive it is to acquire fluorescent and chemiluminescent gels and blots on the ChemiDoc systems. This easy-to-use software is perfect for multi-group use and comes installed on both the ChemiDoc and ChemiDoc MP.

Learn More about Image Lab Touch Software »


Included Components

  • Internal computer with Image Lab Touch Software
  • 12” touch screen display
  • Image Lab Software to install on your computer
  • Blot/UV/Stain-Free Sample Tray for Bio-Rad stain-free gels and blots, fluorescence multiplexing, chemiluminescence detection, ethidium bromide and SYPRO Ruby (12003028)

Mini and Midi ChemiDoc MP Stain-Free Western Blotting also include:

  • ChemiDoc MP Imaging System
  • Mini-PROTEAN Tetra Cell, 4-gel (1658004) or Criterion Cell (1656001) Electrophoresis Chamber
  • PowerPac Basic Power Supply (1645050)
  • Trans-Blot Turbo Transfer System (1704150)
  • 4–20% Mini-PROTEAN TGX Stain-Free or 4–20% Criterion TGX Stain-Free Protein Gels
  • Buffers, Nitrocellulose Transfer Packs, Immun-Blot LF PVDF Membranes, and other consumables

Optional Components

  • White Sample Tray for Coomassie Blue, copper, silver, and zinc stains (12003026)
  • Blue Sample Tray for nucleic acid applications with GelGreen or any SYBR Stain (12003027)
  • Holder for ChemiDoc Sample Trays and UV Shield (1708377)
  • Gel Alignment Templates for ChemiDoc MP and ChemiDoc Imaging Systems (12003915)
  • UV Safety Shield for ChemiDoc MP and ChemiDoc Imaging Systems (12003914)

Configuration Options

Catalog Number
ChemiDoc MP Imaging System
ChemiDoc MP Stain-Free Western Blotting Workflow for Mini Gels
ChemiDoc MP Stain-Free Western Blotting Workflow for Midi Gels


Note: Also available are the ChemiDoc Imaging System (17001401), ChemiDoc Stain-Free Western Blotting Workflows for Mini Gels (17001403), and ChemiDoc Stain-Free Western Blotting Workflows for Midi Gels (17001390), which have a more limited fluorescence capability than ChemiDoc MP Systems. These systems can be upgraded to a broader spectrum (full RGB, far red, and near IR) to enable fluorescence multiplexing with the ChemiDoc to ChemiDoc MP Upgrade Kit (12003263).

Related Categories

ChemiDoc MP Imager

Important Information

The ChemiDoc MP Imaging System featured on this page replaces our classic model of the ChemiDoc MP Imager, which is being discontinued. However, we will continue to provide support for customers with the discontinued model.




Automation Capabilities
Smart Tray Technology™
Automatic recognition of application-specific tray and adjustment of imaging parameters and software options
Precalibrated focus for any zoom setting or sample height
Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging applications
Image flat fielding
Dynamic; precalibrated and optimized for each application
Touch-screen functionality
Multitouch capability (2 points) 12.1" display
Maximum image area (W x H)
21 x 16.8 cm
Cooled CCD, 6 megapixels
Dynamic range
>4 orders of magnitude
Illumination sources
Trans-UV, 302 nm excitation
Trans-white (requires White Sample Tray)
Trans-blue, 450−490 nm excitation (requires Blue Sample Tray)
Epi-blue, 460−490 nm excitation
Epi-green, 520−545 nm excitation
Epi-red, 625−650 nm excitation
Epi-far red, 650−675 nm excitation
Epi-near IR, 755−777 nm excitation
Filter and application
590/110 nm standard filter to perform protein and DNA gel and blot imaging
Chemiluminescence filter
518–546 nm filter for blue-excitable fluorophores and stains
577–613 nm filter for green-excitable fluorophores and stains
675–725 nm for red-excitable fluorophores and stains
700–730 nm filter for far red-excitable fluorophores and stains
813–860 nm for near IR-excitable fluorophores and stains
Data output
16-bit or 8-bit: SCN, TIFF, JPEG files
Instrument weight
35 kg (78 lb)
Instrument size (L x W x H)
61 x 51 x 53 cm
Operating voltage
100–250 V
Operating temperature
Operating humidity
10–85% relative humidity (non-condensing)




Use the filters below to refine results!


The ChemiDoc MP Imaging System is a full-feature, best-in-class instrument for imaging and quantifying nucleic acid and protein in gels and western blots. It is capable of imaging fluorescent western blots (RGB, far-red, near-IR), chemiluminescent western blots, all common nucleic acid and protein gel stains, plus stain-free gels. Includes internal computer, 12" touch-screen display, camera, Image Lab™ Touch Software, and Image Lab Software


USB flash drive containing Image Lab Touch Software version 3.0.1 upgrade for use with Bio-Rad ChemiDoc and ChemiDoc MP Imaging Systems. Find release notes​ and installation instructions here.



Pkg of 1, sample tray for gels and blots stained with Coomassie Blue, copper, silver, or zinc stains
Pkg of 1, sample tray for nucleic acid applications with GelGreen or any SYBR® Stain
Pkg of 1, sample tray for imaging chemiluminescence and fluorescence blots, stain-free gels and blots, and gels stained with ethidium bromide, SYPRO Ruby, Oriole, or SYBR® Stains
Pkg of 1, UV safety shield
This kit ensures your ChemiDoc and ChemiDoc MP Instruments are properly installed and calibrated according to the technical specifications for optimal performance according to regulatory guidelines

Pkg of 1, goggles for use with SYBR Safe dyes and Blue Trays for use with ChemiDoc and GelDoc Imaging Systems


Pkg of 1, imaging tray holder, holds 3 sample trays and 1 UV shield for ChemiDoc and GelDoc Imaging Systems; trays and shield not included

Pkg of 2, alignment templates for use with ChemiDoc MP and ChemiDoc Imaging Systems

Optional thermal printer, for use with Bio-Rad imaging systems


Pkg of 4, printer paper rolls for use with Mitsubishi Thermal Printer (#170-8089)

Pkg of 6, 302 nm replacement UV lamps for all ChemiDoc™ and Gel Doc™ Imaging Systems



Number Description Options
Traditional vs. Stain-Free Western Blotting
Stain-Free Western Blotting Brochure
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General Stain-Free Western Blotting Protocol
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Imaging Systems Family Flier
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ChemiDoc MP Imaging System Brochure
ChemiDoc MP Imaging System Flier, Ver A
ChemiDoc and ChemiDoc MP Imaging Systems with Image Lab Touch Software Use Guide Version 3.0.1 (English)
ChemiDoc and ChemiDoc MP Imaging Systems with Image Lab Touch Software Use Guide Version 3.0.1 (Simplified Chinese)
Bio-Rad Stain-Free Western Blotting
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Acquiring an Image with ChemiDoc™ and ChemiDoc MP Imaging Systems Quick Guide, Ver A
Acquiring a Multichannel Image with the ChemiDoc™ and ChemiDoc MP Imaging Systems Quick Guide, Ver B
Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide
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