CFX96 Touch Real-Time PCR Detection System

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CFX96 Touch Real-Time PCR Detection System

Overview

1000-series Touch Family System Tour
Flexibility

The Ultimate in Configuration Flexibility

A System That Grows with Your Research
The flexibility of the 1000-series platform allows your setup to grow and evolve as your needs change. Adding more instruments to increase throughput or expand options is as easy as connecting a USB cable. CFX Manager™ software can independently run four instruments, including a combination of CFX96 Touch™, CFX96 Touch Deep Well™, or CFX384 Touch™ real-time instruments and C1000 Touch™ or S1000™ thermal cyclers. You may start a run on one instrument while another is running a different experiment, or you can start the same experiment on all four instruments at once.

CFX96 Connections

Configuration Options

Stand-Alone

  • Stand-alone thermal cyclers and real-time PCR detection systems

 


Stand-Alone Multi-Instrument

  • C1000 Touch cycler control of up to 3 S1000 cyclers
  • CFX96 Touch, CFX96 Touch Deep Well, or CFX384 Touch system control of up to 3 S1000 cyclers

 


Software-Controlled Stand-Alone

  • C1000 Touch cycler or CFX96 Touch, CFX96 Touch Deep Well, or CFX384 Touch system controlled by CFX Manager software

 


Software-Controlled Multi-Instrument

  • Up to 8 C1000 Touch cyclers, each connected to 1–3 S1000 cyclers, controlled by CFX Manager software
  • 1 CFX96 Touch, CFX96 Touch Deep Well, or CFX384 Touch system connected to 1–3 S1000 cyclers, controlled by CFX Manager software
  • Up to 4 CFX96 Touch, CFX96 Touch Deep Well, and/or CFX384 Touch systems controlled by CFX Manager software

 

Performance

 

Excellent uniformity. IL-1β plasmid template diluted to 105 copies/reaction amplified in the presence of a FAM-labeled detection probe with iQ™ supermix. Graph shows 96 replicates of 10 µl reactions. Average quantification cycle (Cq) = 19.81 ± 0.10. RFU, relative fluorescence units.

 

Confidently analyze data from a broad range of sample concentrations even when multiplexing five targets. A–E, fluorescence data from a series of tenfold dilutions of plasmid DNA (108–102 copies) amplified using reporter dyes to monitor five targets: , FAM/actin; , HEX/GAPDH; , Texas Red/cyclophilin; , Cy5/tubulin; , Quasar 705/IL-1β; F, standard curves generated from data in A–E, reaction efficiencies range from 97 to 103%. Cq, quantification cycle; RFU, relative fluorescence units.

Plastics Compatibility Chart

Instrument Compatibility of PCR Plastic Consumables

 

Real-Time PCR Detection Systems
  MiniOpticon System
MiniOpticon™ System
MyiQ2 and iCycler iQ Systems
iCycler iQ®, iQ™5,
MyiQ™, and MyiQ™2 Systems
Chromo4 System
Chromo4™ System
DNA Engine Opticon 2 System
DNA Engine Opticon® 2 System

CFX96 Touch™, CFX96 Touch Deep Well™, CFX384
Touch™, and CFX Connect™ Systems
Tubes
High-profile individual tubes   CFX96 Touch
Deep Well only
Low-profile tube strips  
48- and 96-Well Plates
Hard-Shell® low-profile 96-well semi-skirted PCR plates        
Hard-Shell high-profile 96-well semi-skirted PCR plates     CFX96 Touch
Deep Well only
Hard-Shell low-profile 96-well skirted PCR plates    
Multiplate™ high-profile unskirted PCR plates   CFX96 Touch
Deep Well only
Multiplate low-profile unskirted PCR plates  
iQ™ 96-well semi-skirted PCR plates      
384-Well Plates
Hard-Shell 384-well skirted PCR plates        
Lids and Adhesive Seals
Optical flat cap strips
Microseal® 'B' adhesive seals  
Microseal® ꞌCꞌ optical seals  
Heat Sealer and Optically Clear Heat Seal
PX1™ PCR plate sealer  
Optically clear heat seal  
qPCR Applications Guide
qPCR Applications Guide

The qPCR Applications Guide is a comprehensive source of information on real-time PCR. Complete with detailed information on optimizing, validating, and analyzing real-time PCR data, this technically focused applications guide is a resource for experienced researchers as well as those wanting to learn more about using real-time PCR for applications such as qPCR, RT-qPCR, genotyping, and GMO detection. This guidebook includes chapters that cover:

  • Key concepts of real-time PCR
  • Getting started — experimental design
  • Multiplexing considerations
  • Data analysis
  • Gene expression analysis
  • Genotyping allelic discrimination
  • GMO detection

This guidebook comes with an easy-to-review real-time PCR quick guide to use at the bench. The quick guide covers the fundamentals of qPCR, design, optimization, and data analysis of qPCR experiments.

The CFX96 Optical Reaction Module converts a C1000 Touch Thermal Cycler into a real-time PCR system. This six-channel system combines advanced optical technology with precise thermal control.
Read full description

Ordering Information


  • Description
  • Specifications
  • Ordering
  • Accessories
  • Kits & Reagents
  • Download
  • Plastics
  • Documents

Description

Request a Demo
 
CFX System

Real-Time PCR Tips, Tricks, and Best Practices Guide

Get the Guide »

The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions.

Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR.

Key Features and Benefits

With the CFX96 Touch Real-Time PCR Detection System you can:

  • Set up your system quickly — easy installation and factory-calibrated optics
  • Minimize sample and reagent use — up to 5-target multiplexing with sample volumes as low as 10 µl
  • Optimize reactions in a single run — thermal gradient feature
  • Analyze data faster — visualize all run data at once and export only the data you need in the format you want
  • Use advanced data analysis tools — normalized gene expression using CFX Maestro Software
  • Configure the system to fit your needs — run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX Automation System II for higher throughput
  • Combine the CFX96 Touch System with good laboratory practice standards — use CFX Maestro Software, Security Edition for data collection and analysis to simplify compliance with U.S. FDA 21 CFR Part 11 regulations

Flexible CFX System Configurations Meet Your Throughput Needs

The CFX Automation System II works with all CFX Real-Time PCR Detection Systems to enable walk-away, high-throughput qPCR operation. Integrate this robotic plate handler with one or two CFX Systems to maximize throughput while maintaining a compact footprint.

Flexible Configurations Meet Your Throughput Needs

CFX Maestro Software Works Seamlessly with PrimePCR Assays and Panels

The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Maestro Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.

  • Begin analyzing data in a single step by importing target, reference, and control information from a PrimePCR run file directly into CFX Maestro Software
  • Combine data from multiple plates into a Gene Study to rapidly screen large numbers of targets or samples
  • Use powerful data visualization tools, such as hierarchical clustering and color-matched expression levels, to identify individual targets or clusters to consider for further investigation

Service Plans and Validation Tools for CFX Systems, Thermal Cyclers, and Real-Time PCR Instruments


Specifications

Thermal Cycler  
Chassis C1000 Touch™
Maximum ramp rate, °C/sec 5
Average ramp rate, °C/sec 3.3
Heating and cooling method Peltier
Lid, °C Heats up to 105
Temperature  
Range, °C 0–100
Accuracy, °C ±0.2 of programmed target at 90°C
Uniformity, °C ±0.4 well-to-well within 10 sec of arrival at 90°C
Gradient  
Operational range, °C 30–100
Programmable span, °C 1–24
Optical Detection  
Excitation 6 filtered LEDs
Detection 6 filtered photodiodes
Range of excitation/emission wavelengths, nm 450–730
Sensitivity Detects 1 copy of target sequence in human genomic DNA
Dynamic range 10 orders of magnitude
Scan Time  
All channels, sec 12
FAM/SYBR® Green only, sec 3
Software  
Operating systems Windows 7, Windows 8, Windows 10
Multiplex analysis Up to 5 targets per well
System  
Licensed for real-time PCR Yes
Sample capacity, wells 96
Sample size, µl 1–50 (10–25 recommended)
Communication interface USB 2.0
Electrical approvals IEC, CE
Dimensions (W x D x H), cm/in 33 x 46 x 36 /13 x 18 x 14
Weight, kg/lb 21/47

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CFX96 Optical Reaction Module for Real-Time PCR Systems with Starter Package

1845096
96–well optical module for real-time PCR, includes CFX Maestro Software, qbase+ Software license, cables, reagents, consumables, for use with C1000 Touch™ Thermal Cycler Chassis

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CFX96 Optical Reaction Module for Real-Time PCR Systems

1845097
96-well optical module for real-time PCR, includes cables, for use with C1000 Touch Thermal Cycler Chassis

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CFX Qualification Plate

1845098
Predispensed 96-well plate for performance validation of CFX96™, CFX96 Touch™, CFX96 Touch™ Deep Well, or CFX Connect™ Real-Time PCR Detection System, includes template DNA, primers, supermix, water

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$692.00 
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PX1 PCR Plate Sealer

1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

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$4,305.00 
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(Discontinued) 1000-Series Connectivity Kit

1841001
Kit contains mouse, mouse pad, USB flash drive

This product is no longer available. Please contact technical support for more information on replacement product.

Kits & Reagents

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SsoAdvanced™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725271
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions

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$411.00 
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SsoAdvanced Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725281
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions

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$400.00 
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iTaq™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725121
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, and ROX Normalization Dyes

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$332.00 
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iTaq™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725131
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, enhancers, stabilizers, and ROX Normalization Dyes

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$332.00 
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SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml

1725160
1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions

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$749.00 
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SingleShot™ SYBR® Green Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

1725085
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions

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$1,316.00 
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iScript™ Advanced cDNA Synthesis Kit, 100 x 20 µl rxns

1725038
100 x 20 µl reactions, includes 400 µl 5x iScript Advanced Reaction Mix, 100 µl iScript Advanced Reverse Transcriptase

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$676.00 
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iScript™ Reverse Transcription Supermix, 100 x 20 µl rxns, 400 µl

1708841
100 x 20 µl reactions, 4 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix

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$562.00 
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CFX96™ and CFX384™ Real-Time PCR Detection Systems Firmware Update

SOFT-CFX-FW
Ensures optimal performance from your instrument. Version HC12 1.0.176, PXA270 3.3.155.524, DSP 1.124, and 8051 Smartlid 0.54

Precision Melt Analysis™ v1.2 Software Upgrade

SOFT-PM-12-PATCH
Upgrade Precision Melt Analysis Software from version 1.0 or 1.1 to version 1.2 for use with CFX Manager™ Software version 2.0 and higher


64-Bit OS PC Patch

SOFT-64-PATCH
Patch installs Windows 64-bit operating system PC drivers compatible with CFX96™ and CFX384™ real-time PCR detection systems controlled by CFX Manager™ software



Plastics

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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear

HSP9601
Pkg of 50, white shell/clear well PCR plate, rigid 2-component design

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$276.00 
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear, barcoded

HSP9901
Pkg of 50, white shell/clear well PCR plate, barcoded, 7-digit sequential, Code 128, rigid 2-component design

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$287.00 
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Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/white

HSP9655
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

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$298.00 
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Multiplate™ 96-Well PCR Plates, low profile, unskirted, clear

MLL9601
Pkg of 25, clear PCR plate, one-component polypropylene, can be cut for fewer samples

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$123.00 
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Multiplate™ 96-Well PCR Plates, low profile, unskirted, white

MLL9651
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

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$141.00 
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Microseal 'B' PCR Plate Sealing Film, adhesive, optical

MSB1001
Pkg of 100, optically clear seal for PCR plates

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$191.00 
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0.2 ml 8-Tube PCR Strips without Caps, low profile, clear

TLS0801
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, clear (960 tubes)

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$123.00 
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0.2 ml 8-Tube PCR Strips without Caps, low profile, white

TLS0851
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)

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$130.00 
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0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear

TCS0803
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear

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$43.00 
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Documents

Number Description Options
7444 A Model qPCR Instrument Comparability Study: CFX96 Touch and CFX Opus 96 Real-Time PCR Systems Click to download
7180 CFX96 Touch Protein Thermal Shift Assay Protocol Click to download
6093 CFX96 Touch™ Real-Time PCR Detection System Brochure Click to download [ Add to Cart (Free) ]
6894 Tips, Tricks & Best Practices: The Ultimate qPCR Assay Design Guide Click to download [ Add to Cart (Free) ]
10000068706 C1000 Touch™ Thermal Cycler and CFX Real-Time PCR Detection Systems Instrument Guide, Ver A Click to download
6900 CFX Maestro Software Brochure Click to download [ Add to Cart (Free) ]
10000068703 CFX Maestro Software User Guide Click to download
10000115381 CFX96 Touch and CFX96 Touch Deep Well Real-Time PCR Detection Systems Installation Quick Guide, Ver D Click to download
10027341 CFX Qualification Plate — 96-Well Plate Format Product Insert Click to download
10000115384 Checking Firmware and Software Updates for the C1000 Touch Thermal Cycler and the CFX96 Touch, CFX96 Touch Deep Well, and CFX384 Touch Real-Time PCR Detection Systems, Quick Guide, Ver D Click to download
6144 Plate Preparation for the CFX384™ Real-Time PCR Detection System Using a Tecan Robotics System, Rev A Click to download
6149 Plate Preparation for the CFX384™ Real-Time PCR Detection System Using the IDEX Health & Science Innovadyne Dispenser, Rev A Click to download
5690 CFX Manager™ Software, Security Edition Product Information Sheet, Rev A Click to download
10022527 Amplification Plastics and Reagents Selection Guide Click to download
5990 Gene Expression Analysis Solutions Brochure Click to download
6629 High-Throughput Real-Time PCR Solutions Brochure Click to download
5279 Real-Time PCR Applications Guide, Rev B Click to download
7216 End the Cycle of Repeated, Failed Experiments with a Robust Field Guide to qPCR Click to download
6047 Optical Design of CFX96™ Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye, Rev A Click to download
5692 Real-Time qPCR as a Tool for Evaluating RNAi-Mediated Gene Silencing, Rev A Click to download
5962 Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient, Rev A Click to download
6634 A Fast Semi-Automated Quantitative PCR Method for Monitoring Differential Gene Expression for Drug Target Discovery, Ver B Click to download
6763 Gene Expression Real-Time PCR Workflow Flier Click to download
6777 Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR Click to download

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