CFX Duet Real-Time PCR System

CFX Duet Real-Time PCR System

The accuracy and consistency of Bio-Rad qPCR accessible for single and duplex experiments.

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Overview

CFX Duet Real-Time PCR System makes the power and consistency of Bio-Rad qPCR available to almost any lab. The CFX Duet System is the fresh modern system for small and more economical labs with its improved thermal performance and proprietary, accurate optical shuttle system.

Features and Benefits

  • Quick system set up — easy installation and factory-calibrated optics
  • Our best thermal uniformity and accuracy to date
  • Uniform optical system free of regular calibration requirements
  • Audit and e-signature compatibility with CFX Maestro Security Edition Software
  • Eliminates the need for a reference/normalizing dye the CFX Duet System can perform duplex assays including genotyping

Applications and Uses

  • Gene expression without requiring ROX
  • End-point analysis for sequence detection
  • Protein thermal shift assays
  • Assay optimization with thermal gradient
  • Genotyping

Related Products

CFX Maestro Software Works Seamlessly with PrimePCR Assays and Panels

Not only is the CFX Maestro Software is easy to use for routine gene expression and detection experiments, but also integrates with our PrimePCR Assays and Panels. The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Maestro Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.

  • Begin analyzing data in a single step by importing target, reference, and control information from a PrimePCR Assay run file directly into CFX Maestro Software
  • Combine data from multiple plates into a gene study to rapidly screen large numbers of targets or samples
  • Use powerful data visualization tools, such as hierarchical clustering and color-matched expression levels, to identify individual targets or clusters to consider for further investigation

Service Plans and Validation Tools for CFX Systems, Thermal Cyclers, and Real-Time PCR Instruments

Specifications

Thermal Cycler
 
Maximum ramp rate, °C/sec
5
Average ramp rate, °C/sec
3.3
Lid, °C
30–110
Temperature
Accuracy, °C
±0.2 of programmed target at 90°C
Uniformity, °C
±0.3 well-to-well after 10 sec of arrival at target
Gradient
Operational range, °C
30–100
Programmable span, °C
1–24
Optical Detection
Excitation
3 filtered LEDs
Detection
3 filtered photodiodes
Range of excitation/emission wavelengths, nm
450–580
Sensitivity
Detects 1 copy of target sequence in human genomic DNA
Dynamic range
10 orders of magnitude
Scan Time
All channels, sec
12
FAM/SYBR® Green only, sec
3
Software
Operating system
Windows 10 and up
Multiplex analysis
Up to 2 targets per well
System
Sample capacity, wells
96
Sample size, µl
1–50 (10–50 recommended)
Communication interface
USB 2.0
Electrical approvals
IEC, CE
Dimensions (W x D x H), cm/in
33x56x36 / 13x22x14
Weight, kg/lb
22/48

Ordering

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CFX Duet Real-Time PCR System
(Discontinued) CFX Duet Real-Time PCR System
12016265

A PCR detection system with 96-well block and 2-color detection plus FRET. Includes CFX Duet base unit and cables.

cfx-maestro-software-12004110-tn.jpg
(Discontinued) CFX Maestro Software 2.3 for Windows PC
12013758

1 license, CFX Maestro 2.3, software for real-time PCR plate setup, data collection, statistics, and graphing of results; for Windows PCs

Accessories

cfx-validation-plate-SKU-184-5098-thumb.jpg
(Discontinued) CFX Qualification Plate
1845098
Predispensed 96-well plate for performance validation of CFX96™, CFX96 Touch™, CFX96 Touch™ Deep Well, or CFX Connect™ Real-Time PCR Detection System, includes template DNA, primers, supermix, water

Kits & Reagents

ssoadvanced-universal-sybr-green-supermix-172-5271-thumb.jpg
(Discontinued) SsoAdvanced™ Universal SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725271
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions
ssoadvanced-universal-probes-supermix-172-5281-thumb.jpg
(Discontinued) SsoAdvanced Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725281
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions

Plastics

HSP9655_tn.jpg
(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/white
HSP9655
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
msb-1001_tn.jpg
(Discontinued) Microseal 'B' PCR Plate Sealing Film, adhesive, optical
MSB1001

Pkg of 100, optically clear seal for PCR plates. Note: This item is now available under the new material number MSB1001B. For future orders please proceed here.

Documents

Number Description Options
3277
CFX Duet Real-Time PCR System Brochure
[ Add to Cart (Free) ]
3281
CFX Duet Real-Time PCR System Flier
[ Add to Cart (Free) ]
3283
CFX Duet Real-Time PCR System Product Information Sheet
10000148048
CFX Duet Real-Time PCR System Instrument Guide
10000147782
CFX Duet Real-Time PCR System Quick Start Guide