Cell Lysis RT-qPCR Kits

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Overview

SingleShot Cell Lysis RT-qPCR Kits allow RT-qPCR to be performed directly from cultured cells without RNA purification. Kits are available for both one-step and two-step RT-qPCR with SYBR Green and probe chemist

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Description

Description

Single Shot Workflow

SingleShot Cell Lysis RT-qPCR Kits provide a complete and fast solution for the generation of lysates from cell cultures, optimized for downstream one step or two step quantitative PCR (qPCR) reactions, without an RNA purification step.

Kits are available in SYBR Green or probe chemistries. A cell lysis kit is also offered as a stand-alone option.

All kits offer the following key benefits:

Choose the Right Kit for Your Needs

SingleShot Kit Features Product Options
Cell Lysis One-Step RT-qPCR Kits
  • Cell lysis, reverse transcription, and qPCR
  • All one-step RT-qPCR reagents included
  • No RNA purification needed
SYBR Green
100 reactions (1725095)
Probes
100 reactions (1725070)
Cell Lysis Two-Step RT-qPCR Kits
  • Cell lysis, reverse transcription, and qPCR
  • All two-step RT-qPCR reagents included
  • No RNA purification needed
SYBR Green
100 reactions (1725085)
Probes
100 reactions (1725090)
Cell Lysis Kits 100 reactions (1725080)
500 reactions (1725081)

 

Simple Workflow

Simple, short, minimal hands-on protocol is suitable for automation and provides a high-throughput solution.

Grow cells to desired density, transfer up to 105 cells to wells of a PCR plate, add lysis solution, and incubate at room temperature for 10 minutes.

Heat for 5 minutes at 37°C and then for 5 minutes at 75°C. The lysate is ready for RT-qPCR or can be stored for future use.

Preamp Single Shot Workflow

Cell Lines Validated for Use with the SingleShot Cell Lysis RT-qPCR Kits

All SingleShot Cell Lysis RT-qPCR Kits have been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:

Cell Line Property Species
A549 Adherent H. sapiens, lung carcinoma
HeLa Adherent H. sapiens, cervical adenocarcinoma
HepG2 Adherent H. sapiens, liver carcinoma
NIH/3T3 Adherent M. musculus, embryonic fibroblast
MCF-7 Adherent  H. sapiens, epithelial, adenocarcinoma
NCI-H1975 Adherent H. sapiens, lung adenocarcinoma 
K-562 Suspension H. sapiens, chronic myelogenous leukemia
HEK 293 Suspension H. sapiens, kidney
Jurkat Suspension H. sapiens, T lymphocyte
PC-12 Suspension R. norvegicus, pheochromocytoma, adrenal
HepRG Primary-like H. sapiens, hepatocytes

 

Related Categories

 

 

Supporting Documentation

Online Resources

Specifications

Specifications

Storage at 4°C
6 months:
Lysis buffer

3 months:
iTaq™ Universal One-Step Reaction Mix
SsoAdvanced™ Universal Supermix
Storage at –20°C
All kit components are stable for 12 months

Ordering

Ordering

items

Use the filters below to refine results!

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1725080

Pkg of 1, kit includes 5 ml (1 x 5 ml) of cell lysis buffer, 100 µl of proteinase K solution, and 100 µl of DNase solution, for 100 x 50 µl reactions.

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1725081
Pkg of 1, kit includes 25 ml (5 x 5 ml) of cell lysis buffer, 500 µl of proteinase K solution, and 500 µl of DNase solution, for 500 x 50 µl reactions
singleshot-sybr-green-kit-172-5085-thumb.jpg
1725085
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions
single-shot-probes-kit-172-5090-thumb.jpg
1725090
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal Probes Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions
singleshot-sybr-green-one-step-kit-172-5095-thumb.jpg
1725095
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq™ Universal SYBR® Green One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions
singleshot-probes-one-step-kit-172-5070-thumb.jpg
1725070
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq™ Universal Probes One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions

Documents

Documents

Number Description Options
10000102577
SingleShot SYBR Green One-Step Kit
10042473
User Manual, SingleShot™ Probes Kit, Rev A
6774
Parallel Analysis of RNA and Protein Expression Using SingleShot™ Cell Culture Lysates Application Note, Ver A
6776
Protocol for Parallel Analysis of RNA and Protein Expression Using SingleShot™ Cell Culture Lysates, Ver A
6777
Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR
10042475
User Manual, SingleShot™ SYBR® Green One-Step Kit, Rev A
10042476
User Manual, SingleShot™ Probes One-Step Kit, Rev A
10000112808
User Manual, Singleshot Cell Lysis Kit
6090
Amplification Reagents and Plastics Brochure
[ Add to Cart (Free) ]
6629
High-Throughput Real-Time PCR Solutions Brochure
6604
SingleShot™ Cell Lysis RT-qPCR Kits Application Note, Rev B
[ Add to Cart (Free) ]
6572
Accurate and Reproducible RT-qPCR Gene Expression Analysis on Cell Culture Lysates Using the SingleShot™ SYBR® Green Kit, Rev A
6833
High Throughput Single Cell Gene Expression Profiling by Multiplex qPCR, Ver A