Bio-Scale™ Mini UNOsphere SUPrA™ Resin Cartridges

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Bio-Scale™ Mini UNOsphere SUPrA™ Resin Cartridges

Overview

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 7324120 7324122 7324124
UNOsphere Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 7324100 7324102 7324104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 7324110 7324112 7324114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 7324140 7324142 7324144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 7324130 7324132 7324134
IgG Purification
DEAE Blue DEAE Affi-Gel Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

7324632 7324634
Antibody Purification
Protein A UNOsphere SUPrA Affinity Recombinant protein A 30 mg/ml human IgG 7324200, 1 x 1 ml 7324201, 5 x 1 ml 7324202, 1 x 5 ml
Protein A Affi-Prep Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
7324600 7324602
CHT Type I CHT Ceramic
Hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 7324322 7324324
CHT Type II CHT Ceramic
Hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 7324332 7324334
Affinity-Tagged Protein Purification
IMAC** Nuvia IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
7800811 7800812
IMAC** Profinity IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
7324610 7324612 7324614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
7324620 7324622 7324624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
7324642 7324644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel P-6 Gel filtration Polyacrylamide 10–22% CV*** 7324502 7324504 7325304 7325312 7325314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

UNOsphere SUPrA Cartridges are available in 1 ml and 5 ml capacities for protein A–based affinity chromatography. They deliver high binding capacity at a wide range of linear velocities and bed heights.
Read full description

Ordering Information


Description

UNOsphere SUPrA Resins were developed for protein A–based affinity chromatography and designed to provide a balance between dynamic binding capacity, flow properties, and stability. These prepacked protein A resin cartridges provide flexibility and a predictable scale-up path.

  • Available in 1 and 5 ml cartridges
  • Designed for method development
  • Delivers high binding capacity at a wide range of linear velocities and bed heights
  • Combined with UNOsphere Q and CHT Type I ceramic hydroxyapatite media, UNOsphere SUPrA media complete the purification workflow solution for monoclonal antibodies

More Information

High Performance Columns For Antibody and Tagged Protein PurificationsLearn more about Bio-Rad’s suite of high-performance chromatography columns designed for high-resolution laboratory scale protein purification.







Related Products

Fitting adaptors are available for connection to other low- and medium-pressure chromatography systems:

  • Fittings kit for connection to BioLogic DuoFlow™ Systems (732-0113)
  • Fittings kit for connection to older AKTA systems with M6 fittings (732-0111)
  • Fittings kit for connection to NGC™ Systems and newer AKTA systems with 1/16th inch/10-32 fittings (732-0112)

Bio-Rad chromatography systems:

 * U.S. patent 7,208,087.


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Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 1 ml

7324200
1 ml, prepacked, UNOsphere SUPrA affinity media cartridge

This product is being discontinued soon. Please contact customer service for more information.
Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 5 x 1 ml

7324201
Pkg of 5, 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit UNOsphere SUPrA Columns, 5 x 1 ml (12009322)
Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 5 ml

7324202
5 ml, prepacked, UNOsphere SUPrA affinity media cartridge

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit UNOsphere SUPrA Column, 1 x 5 ml (12009323)
1 ml Empty Bio-Scale™ Mini Cartridges

7324660
Pkg of 5, 1 ml, empty chromatography columns, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.
5 ml Empty Bio-Scale™ Mini Cartridges

7324661
Pkg of 5, 5 ml, empty chromatography columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

Documents

Number Description Options
10014997 Instruction Manual, Bio-Scale™ Mini UNOsphere SUPrA™ cartridges, 1 and 5 ml, Rev B Click to download
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
6484 High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
6745 Automated mAb Workflows: Combining Multidimensional (Multi-D) Purifications with Product Analysis, Ver C Click to download
6770 Automated mAb Workflows: Combining Multidimensional (Multi-D) Purifications with Product Analysis (Poster), Ver A Click to download

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