Bio-Scale™ Mini UNOsphere™ Rapid S Cartridges

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Bio-Scale Mini UNOsphere Rapid S Cartridges

Overview

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 7324120 7324122 7324124
UNOsphere Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 7324100 7324102 7324104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 7324110 7324112 7324114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 7324140 7324142 7324144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 7324130 7324132 7324134
IgG Purification
DEAE Blue DEAE Affi-Gel Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

7324632 7324634
Antibody Purification
Protein A UNOsphere SUPrA Affinity Recombinant protein A 30 mg/ml human IgG 7324200, 1 x 1 ml 7324201, 5 x 1 ml 7324202, 1 x 5 ml
Protein A Affi-Prep Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
7324600 7324602
CHT Type I CHT Ceramic
Hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 7324322 7324324
CHT Type II CHT Ceramic
Hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 7324332 7324334
Affinity-Tagged Protein Purification
IMAC** Nuvia IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
7800811 7800812
IMAC** Profinity IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
7324610 7324612 7324614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
7324620 7324622 7324624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
7324642 7324644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel P-6 Gel filtration Polyacrylamide 10–22% CV*** 7324502 7324504 7325304 7325312 7325314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

UNOsphere Rapid S cartridges are available in 1 ml and 5 ml capacities, possess enhanced chemistries to overcome pH shifts during conductivity transitions, and are optimized for high speed and throughput.
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Ordering Information


Description

Prepacked Bio-Scale™ Mini cartridges have a patented* double-wall cartridge design that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with aqueous buffers most commonly used for protein separation. The cartridges are prepacked with media for ion exchange, affinity, and hydroxyapatite and fluoroapatite chromatography. The cartridges are convenient, ready to use, and disposable, and the simple luer connections connect easily to any chromatography system (see bulletin 5326 under the Documents tab). Bio-Rad now offers UNOsphere™ Rapid S, UNOsphere SUPrA™, and CFT™ ceramic fluoroapatite media prepacked in Bio-Scale Mini cartridges and two kits: one for purification using apatite (CFT ceramic fluoroapatite and CHT™ ceramic hydroxyapatite) and one for monoclonal antibody purification.

UNOsphere Rapid S strong cation exchange media have enhanced chemistries to overcome the pH shift that occurs with conductivity transitions. The support quickly adjusts to changes in conductivity with minimal pH excursion. No pH shifting provides a new level of economy due to shorter equilibration times, resulting in faster separations and lower consumption of buffer solutions. The media have been optimized to deliver speed and throughput in capture and intermediate purification steps. UNOsphere Rapid S media combine high binding capacity, fast mass transfer, excellent flow characteristics, and robust physical properties to make it an exceptional choice for purification.

  • Available in 1 and 5 ml cartridges
  • Excellent scalability
  • Stable in 1 M NaOH
  • High binding capacity
  • High linear flow rates at low backpressures
  • Rapid regeneration
  • Fully supported for regulatory submissions
  • Engineered for reproducible results

* U.S. patent 7,208,087 B2.


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(Discontinued) Bio-Scale™ Mini UNOsphere™ Rapid S Cartridges, 5 x 1 ml

7324400
Bio-Scale Mini UNOsphere Rapid S Cartridges are no longer available. Please use Bio-Scale Mini UNOsphere S Cartridges, Foresight™ UNOsphere™ S Columns, or Foresight™ Nuvia™ HR-S Columns as alternative products.

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) Bio-Scale™ Mini UNOsphere™ Rapid S Cartridges, 1 x 5 ml

7324401
Bio-Scale Mini UNOsphere Rapid S Cartridges are no longer available. Please use Bio-Scale Mini UNOsphere S Cartridges, Foresight™ UNOsphere™ S Columns, or Foresight™ Nuvia™ HR-S Columns as alternative products.

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) Bio-Scale™ Mini UNOsphere™ Rapid S Cartridges, 5 x 5 ml

7324402
Bio-Scale Mini UNOsphere Rapid S Cartridges are no longer available. Please use Bio-Scale Mini UNOsphere S Cartridges, Foresight™ UNOsphere™ S Columns, or Foresight™ Nuvia™ HR-S Columns as alternative products.

This product is no longer available. Please contact technical support for more information on replacement product.

Documents

Number Description Options
10005454 Instruction Manual, Bio-Scale™ Mini UNOsphere™ Q, S and Rapid S Cartridges, 1 and 5 ml, Rev E Click to download
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
5326 Chromatography Column Connection Guide, Rev A Click to download

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