Bio-Scale™ Mini DEAE Affi-Gel® Blue Cartridges

Image
lsr_bio-scale_mini_cartridges.jpg

Overview

Bio-Scale Mini DEAE Affi-Gel Blue Cartridges are 5 ml of DEAE Affi-Gel Blue Support, a bifunctional affinity gel containing Cibacron Blue F3GA dye covalently attached to DEAE Bio-Gel A.
Read full description Ordering Information

Description

Description

Bio-Scale Mini DEAE Affi-Gel Blue cartridges are 5 ml cartridges filled with DEAE Affi-Gel Blue support. DEAE Affi-Gel Blue gel is a bifunctional affinity gel containing Cibacron Blue F3GA dye covalently attached to DEAE Bio-Gel A. The dye binds albumin, proteases, and other complement proteins, while the DEAE group binds remaining acidic proteins. DEAE Affi-Gel Blue gel offers an economical alternative to protein A affinity chromatography; its features include:

  • Single-step IgG purification from serum (the eluted IgG contains a small amount of transferrin)
  • Minimal sample preparation (no dialysis or desalting)
  • No detectable proteolytic activity in the eluted IgG fraction

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems. Bio-Scale Mini affinity cartridges can also be run on the Profinia protein purification system.

*U.S.patent 7,208,087.

Specifications

Specifications

Sizes
1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate
Varies for each support type*
Maximum operating pressure
1, 5, and 10 ml: 45 psi
Fittings
Female luer inlet and male luer outlet
Column material
Polypropylene
Frit material
Polyethylene (HDPE)
Dimensions
1 ml — 40 mm length x 5.6 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.
 

Ordering

Ordering

items

Use the filters below to refine results!

732-4632_tn.jpg
7324632
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4634_tn.jpg
7324634
Pkg of 5, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4620_tn.jpg
7324660

Pkg of 5, 1 ml, empty chromatography columns, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

732-4624_tn.jpg
7324661
Pkg of 5, 5 ml, empty chromatography columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

Documents

Documents

Number Description Options
6498
Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C
[ Add to Cart (Free) ]
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
10006402
Bio-Scale Mini Affi-Gel® Blue and DEAE Affi-Gel Blue Cartridges, 5 ml, Instruction Manual, Rev C