Bio-Scale Mini CHT Ceramic Hydroxyapatite Cartridges

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Overview

Bio-Scale Mini CHT cartridges are 5 ml cartridges filled with CHT ceramic hydroxyapatite support, a leading purification medium for today's demanding downstream process industry.
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Description

Description

Bio-Scale Mini CHT cartridges are 5 ml cartridges filled with CHT ceramic hydroxyapatite support, a leading purification medium for today's demanding downstream process industry. The mixed-mode support offers unique selectivities and often separates biomolecules that appear homogeneous using other chromatographic methods. The diverse binding capabilities of CHT for host cell proteins, leached protein A, antibody dimers and aggregates, nucleic acids, and viruses allow its use at any stage from initial capture to final polishing.

The cartridges are available with either CHT Type I or Type II, 40 µm media. The Type I support has a protein binding capacity of 25–60 mg of human IgG/ml and higher capacity for acidic proteins. Type II has a protein binding capacity of 15–25 mg of human IgG/ml and gives better resolution of nucleic acids and of proteins that elute early. Type II has very low affinity for albumin, so it is often more suitable than Type I for purification of many species and classes of immunoglobulins.

The robust properties of CHT ceramic hydroxyapatite improve efficiency, yield, and financial value through:

  • Excellent capture at elevated flow rates, enabling processing at all scales
  • Large capacity for high-titer upstream feedstocks
  • Exceptional selectivity, allowing a two-step chromatographic process

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, disposable, and ready to use, these cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system. The simple luer connections can connect easily to any system (request bulletin 5326).

CHT Ceramic Hydroxyapatite Mechanism

Hydroxyapatite contains two types of binding sites, positively charged calcium and negatively charged phosphate groups. These sites are distributed regularly throughout the crystal structure of the matrix. Solute species dominantly interact through cation exchange via the phosphate groups and/or metal affinity via the calcium atoms.

For more information, request Bulletin 5709.

*U.S. patent 7,208,087.

Ordering

Ordering

items

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7324322
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I
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7324324
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I
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7324332
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II
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7324334
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II

Accessories

Accessories

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7320113
Low-pressure system fittings kit, includes 1/4–28 female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow™ and NGC Systems
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7320112
Low-pressure adaptor fittings kit, includes luer to 10-32 fittings to connect 1 cartridge to an HPLC system
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7320111
Low-pressure adaptor fittings kit, includes luer to M6 fittings to connect 1 cartridge to an FPLC system

Documents

Documents

Number Description Options
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
6484
High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B
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6498
Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C
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6786
Monoclonal Antibody Purification Resin Selection Card, Ver A
6789
IgG Polyclonal Antibody Purification Resin Selection Card, Ver A
5326
Chromatography Column Connection Guide, Rev A
5709
How CHT Ceramic Hydroxyapatite Works, Rev A
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5667
CHT Ceramic Hydroxyapatite Product Information Sheet, Rev B
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2156
CHT Ceramic Hydroxyapatite — A New Dimension in Chromatography of Biological Molecules, Rev C
5739
Packing and Slurry-in-Place Procedures for CHT Ceramic Hydroxyapatite Using a Bio-Rad InPlace Process-Scale Chromatography Column, Rev A
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2731
Plasmid Purification Using CHT Ceramic Hydroxyapatite Support, Rev A
2780
Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A
2524
Purification of Horse IgG T Using Macro-Prep DEAE and CHT Ceramic Hydroxyapatite Type I Supports, Rev C
2774
Purification of Transgenic Antibody From Corn Seed Using UNOsphere S and CHT Ceramic Hydroxyapatite Supports, Rev A
1986
Effect of pH on Gradient Elution of Proteins on Two Types of CHT Ceramic Hydroxyapatite, Rev B
1985
Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B.
2940
Removal of Aggregate from an IgG4 Product Using CHT™ Ceramic Hydroxyapatite Resin, Rev B
2849
Protein A Removal From IgG on CHT Ceramic Hydroxyapatite Support, Rev A
2535
Purification of Recombinant Human alpha 1-Antitrypsin From Transgenic Milk Using CHT Ceramic Hydroxyapatite, Rev B
2575
Purification of a Calcium-Binding Protein Using CHT Ceramic Hydroxyapatite
2946
Plasmid DNA Adsorption Behavior on CHT Ceramic Hydroxyapatite Type II Support, Rev A
5662
Process Chromatography Applications Laboratory Brochure, Rev B
6086
Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up
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