Bio-Scale Mini CHT Ceramic Hydroxyapatite Cartridges

Print
Bio-Scale Mini CHT Ceramic Hydroxyapatite Cartridges

Overview

Bio-Scale Mini Cartridge Specifications
Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
50 ml: 40 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml–40 mm length x 5.6 mm inner diameter
5 ml–40 mm length x 12.6 mm inner diameter
10 ml–80 mm length x 12.6 mm inner diameter**
50 ml–80 mm length x 28.2 mm inner diameter**

* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 7324120 7324122 7324124
UNOsphere Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 7324100 7324102 7324104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 7324110 7324112 7324114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 7324140 7324142 7324144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 7324130 7324132 7324134
IgG Purification
DEAE Blue DEAE Affi-Gel Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

7324632 7324634
Antibody Purification
Protein A UNOsphere SUPrA Affinity Recombinant protein A 30 mg/ml human IgG 7324200, 1 x 1 ml 7324201, 5 x 1 ml 7324202, 1 x 5 ml
Protein A Affi-Prep Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
7324600 7324602
CHT Type I CHT Ceramic
Hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 7324322 7324324
CHT Type II CHT Ceramic
Hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 7324332 7324334
Affinity-Tagged Protein Purification
IMAC** Nuvia IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
7800811 7800812
IMAC** Profinity IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
7324610 7324612 7324614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
7324620 7324622 7324624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
7324642 7324644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel P-6 Gel filtration Polyacrylamide 10–22% CV*** 7324502 7324504 7325304 7325312 7325314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Monoclonal Antibody Workflow

Workflow for mAb purification with UNOsphere SUPrA affinity, UNOsphere Q, and CHT Type 1 media.

To read more click here

CHT Specifications
  Type I Type II
Functional groups Ca2+, PO4, OH Ca2+, PO4, OH
Observed dynamic binding capacity lysozyme (Lys) ≥25 mg Lys/g CHT ≥12.5 mg Lys/g CHT
Nominal pore diameter 600–800 A 800–1,000 A
Maximum backpressure 100 bar (1,500 psi) 100 bar (1,500 psi)
Nominal mean particle size 20 ± 2, 40 ± 4, and 80 ± 8 µm 20 ± 2, 40 ± 4, and 80 ± 8 µm
Bulk density 0.63 g/ml 0.63 g/ml
CHT Characteristics
  Type I Type II
Observed dynamic binding capacity IgG 25–60 mg IgG/ml CHT* 15–25 mg IgG/ml CHT**
Typical linear flow rate range 50–1,000 cm/hr  
pH Stability 6.5–14 pH  
Base Stability at least 21 months in 1 N NaOH  
Regeneration 500 mM sodium phosphate, pH7
1,000 mM trisodium phosphate, pH 11–12
 
Autoclavability (bulk) 121°C, 20 min in phosphate buffer, pH 7  
Sanitization 1–2 N NaOH  
Recommended column storage 0.1 M NaOH  
Shelf life (dry, unused material) 85 months stored dry, sealed, and at room temperature  

* 40 µm particles, 300 cm/hr, 5 mM sodium phosphate, pH 6.5
** 40 µm particles, 300 cm/hr, 5 mM sodium phosphate, pH 6.5

IgG Protocol
Purification of IgG Using CHT

Purification of IgG using CHT Ceramic Hydroxyapatite.

To read more click here

Acidic Proteins Protocol Using CHT

Acidic Proteins Protocol using CHT
Optimizing the bioprocess requires management of multiple variables ranging from matrix interaction, elution characteristics, scale-up from bench, regulation and drug safety requirements, and process robustness and economics. This protocol has been developed as general starting guidelines for the purification of most proteins and nucleic acids and may help to reduce time spent in methodology development.

Bio-Scale Mini CHT cartridges are 5 ml cartridges filled with CHT ceramic hydroxyapatite support, a leading purification medium for today's demanding downstream process industry.
Read full description

Ordering Information


  • Description
  • Ordering
  • Accessories
  • Documents

Description

Bio-Scale Mini CHT cartridges are 5 ml cartridges filled with CHT ceramic hydroxyapatite support, a leading purification medium for today's demanding downstream process industry. The mixed-mode support offers unique selectivities and often separates biomolecules that appear homogeneous using other chromatographic methods. The diverse binding capabilities of CHT for host cell proteins, leached protein A, antibody dimers and aggregates, nucleic acids, and viruses allow its use at any stage from initial capture to final polishing.

The cartridges are available with either CHT Type I or Type II, 40 µm media. The Type I support has a protein binding capacity of 25–60 mg of human IgG/ml and higher capacity for acidic proteins. Type II has a protein binding capacity of 15–25 mg of human IgG/ml and gives better resolution of nucleic acids and of proteins that elute early. Type II has very low affinity for albumin, so it is often more suitable than Type I for purification of many species and classes of immunoglobulins.

The robust properties of CHT ceramic hydroxyapatite improve efficiency, yield, and financial value through:

  • Excellent capture at elevated flow rates, enabling processing at all scales
  • Large capacity for high-titer upstream feedstocks
  • Exceptional selectivity, allowing a two-step chromatographic process

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, disposable, and ready to use, these cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system. The simple luer connections can connect easily to any system (request bulletin 5326).

CHT Ceramic Hydroxyapatite Mechanism

Hydroxyapatite contains two types of binding sites, positively charged calcium and negatively charged phosphate groups. These sites are distributed regularly throughout the crystal structure of the matrix. Solute species dominantly interact through cation exchange via the phosphate groups and/or metal affinity via the calcium atoms.

For more information, request Bulletin 5709.

*U.S. patent 7,208,087.


Ordering

x

Account price failed to display. Please try reloading the page.Reload Page

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
email to

Bio-Scale Mini CHT Type I Cartridge

7324322
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit CHT Type I, 40 µm Column, 1 x 5 ml (12009253)
Bio-Scale Mini CHT Type I Cartridges

7324324
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit CHT Type I, 40 µm Columns, 5 x 5 ml (12009254)
Bio-Scale Mini CHT Type II Cartridge

7324332
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit CHT Type II, 40 µm Column, 1 x 5 ml (12009257)
Bio-Scale Mini CHT Type II Cartridges

7324334
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit CHT Type II, 40 µm Columns, 5 x 5 ml (12009258)

Accessories

x

Account price failed to display. Please try reloading the page.Reload Page

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
email to

Luer to BioLogic™ and NGC™ Systems Fittings Kit

7320113
Low-pressure system fittings kit, includes 1/4–28 female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow™ and NGC Systems

List Price: 
 
$68.00 
LoadingLoading...
EDU Price:
 
What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
Luer to 10-32 Adaptor Fittings Kit

7320112
Low-pressure adaptor fittings kit, includes luer to 10-32 fittings to connect 1 cartridge to an HPLC system

List Price: 
 
$111.00 
LoadingLoading...
EDU Price:
 
What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
Luer to M6 Adaptor Fittings Kit

7320111
Low-pressure adaptor fittings kit, includes luer to M6 fittings to connect 1 cartridge to an FPLC system

List Price: 
 
$170.00 
LoadingLoading...
EDU Price:
 
What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 

Documents

Number Description Options
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
6484 High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
6786 Monoclonal Antibody Purification Resin Selection Card, Ver A Click to download
6789 IgG Polyclonal Antibody Purification Resin Selection Card, Ver A Click to download
5326 Chromatography Column Connection Guide, Rev A Click to download
5709 How CHT Ceramic Hydroxyapatite Works, Rev A Click to download [ Add to Cart (Free) ]
5667 CHT Ceramic Hydroxyapatite Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
2156 CHT Ceramic Hydroxyapatite — A New Dimension in Chromatography of Biological Molecules, Rev C Click to download
5739 Packing and Slurry-in-Place Procedures for CHT Ceramic Hydroxyapatite Using a Bio-Rad InPlace Process-Scale Chromatography Column, Rev A Click to download [ Add to Cart (Free) ]
2731 Plasmid Purification Using CHT Ceramic Hydroxyapatite Support, Rev A Click to download
2780 Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A Click to download
2524 Purification of Horse IgGT Using Macro-Prep DEAE and CHT Ceramic Hydroxyapatite Type I Supports, Rev C Click to download
2774 Purification of Transgenic Antibody From Corn Seed Using UNOsphere S and CHT Ceramic Hydroxyapatite Supports, Rev A Click to download
1986 Effect of pH on Gradient Elution of Proteins on Two Types of CHT Ceramic Hydroxyapatite, Rev B Click to download
1985 Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B. Click to download
2940 Removal of Aggregate from an IgG4 Product Using CHT™ Ceramic Hydroxyapatite Resin, Rev B Click to download
2849 Protein A Removal From IgG on CHT Ceramic Hydroxyapatite Support, Rev A Click to download
2535 Purification of Recombinant Human alpha 1-Antitrypsin From Transgenic Milk Using CHT Ceramic Hydroxyapatite, Rev B Click to download
2575 Purification of a Calcium-Binding Protein Using CHT Ceramic Hydroxyapatite Click to download
2946 Plasmid DNA Adsorption Behavior on CHT Ceramic Hydroxyapatite Type II Support, Rev A Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download
6086 Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up Click to download [ Add to Cart (Free) ]

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Get Help
Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.