Bio-Scale™ Mini CHT™ 40 µm Cartridges

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Overview

Bio-Scale Mini CHT Cartridges are 5 ml Bio-Scale Mini Cartridges filled with either CHT Type I or Type II having a particle size of 40 µm.
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Description

Description

Bio-Scale Mini CHT cartridges are 5 ml Bio-Scale Mini cartridges filled with either CHT Type I or Type II having a particle size of 40 µm:

  • Type I — sintered at 400°C; has a high protein binding capacity (>25 mg of lysozyme/g) and higher capacity for acidic proteins
  • Type II — sintered at 700°C; has a lower protein binding capacity (>12.5 mg of lysozyme/g) and gives better resolution of nucleic acids and of proteins that elute early. Type II has very low affinity for albumin, so it is often more suitable than Type I for purification of many species and classes of immunoglobulins

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems.

More Information

High Performance Columns For Antibody and Tagged Protein PurificationsLearn more about Bio-Rad’s suite of high-performance chromatography columns designed for high-resolution laboratory scale protein purification.







Related Products

Fitting adaptors are available for connection to other low- and medium-pressure chromatography systems:

  • Fittings kit for connection to BioLogic DuoFlow™ systems (732-0113)
  • Fittings kit for connection to older AKTA systems with M6 fittings (732-0111)
  • Fittings kit for connection to NGC™ systems and newer AKTA systems with 1/16th inch/10-32 fittings (732-0112)

Bio-Rad chromatography systems:

 * U.S.patent 7,208,087.

Specifications

Specifications

Sizes
1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate
Varies for each support type*
Maximum operating pressure
1, 5, and 10 ml: 45 psi
Fittings
Female luer inlet and male luer outlet
Column material
Polypropylene
Frit material
Polyethylene (HDPE)
Dimensions
1 ml — 40 mm length x 5.6 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.
 

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7324322
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I
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7324324
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I
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7324332
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II
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7324334
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II

Documents

Documents

Number Description Options
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
6498
Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C
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1929
Bio-Scale Ceramic Hydroxyapatite Type I Column Product Information Sheet, Rev A
6484
High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B
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6749
Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes, Ver A
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