Bio-Scale™ Mini CHT™ 40 µm Cartridges

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Bio-Scale Mini CHT 40 μm Cartridges

Overview

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 7324120 7324122 7324124
UNOsphere Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 7324100 7324102 7324104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 7324110 7324112 7324114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 7324140 7324142 7324144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 7324130 7324132 7324134
IgG Purification
DEAE Blue DEAE Affi-Gel Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

7324632 7324634
Antibody Purification
Protein A UNOsphere SUPrA Affinity Recombinant protein A 30 mg/ml human IgG 7324200, 1 x 1 ml 7324201, 5 x 1 ml 7324202, 1 x 5 ml
Protein A Affi-Prep Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
7324600 7324602
CHT Type I CHT Ceramic
Hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 7324322 7324324
CHT Type II CHT Ceramic
Hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 7324332 7324334
Affinity-Tagged Protein Purification
IMAC** Nuvia IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
7800811 7800812
IMAC** Profinity IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
7324610 7324612 7324614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
7324620 7324622 7324624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
7324642 7324644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel P-6 Gel filtration Polyacrylamide 10–22% CV*** 7324502 7324504 7325304 7325312 7325314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Bio-Scale Mini CHT Cartridges are 5 ml Bio-Scale Mini Cartridges filled with either CHT Type I or Type II having a particle size of 40 µm.
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Ordering Information


Description

Bio-Scale Mini CHT cartridges are 5 ml Bio-Scale Mini cartridges filled with either CHT Type I or Type II having a particle size of 40 µm:

  • Type I — sintered at 400°C; has a high protein binding capacity (>25 mg of lysozyme/g) and higher capacity for acidic proteins
  • Type II — sintered at 700°C; has a lower protein binding capacity (>12.5 mg of lysozyme/g) and gives better resolution of nucleic acids and of proteins that elute early. Type II has very low affinity for albumin, so it is often more suitable than Type I for purification of many species and classes of immunoglobulins

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems.

More Information

High Performance Columns For Antibody and Tagged Protein PurificationsLearn more about Bio-Rad’s suite of high-performance chromatography columns designed for high-resolution laboratory scale protein purification.







Related Products

Fitting adaptors are available for connection to other low- and medium-pressure chromatography systems:

  • Fittings kit for connection to BioLogic DuoFlow™ systems (732-0113)
  • Fittings kit for connection to older AKTA systems with M6 fittings (732-0111)
  • Fittings kit for connection to NGC™ systems and newer AKTA systems with 1/16th inch/10-32 fittings (732-0112)

Bio-Rad chromatography systems:

 * U.S.patent 7,208,087.


Specifications

Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml — 40 mm length x 5.6 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.  

Ordering

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Bio-Scale Mini CHT Type I Cartridge

7324322
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit CHT Type I, 40 µm Column, 1 x 5 ml (12009253)
Bio-Scale Mini CHT Type I Cartridges

7324324
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit CHT Type I, 40 µm Columns, 5 x 5 ml (12009254)
Bio-Scale Mini CHT Type II Cartridge

7324332
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit CHT Type II, 40 µm Column, 1 x 5 ml (12009257)
Bio-Scale Mini CHT Type II Cartridges

7324334
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit CHT Type II, 40 µm Columns, 5 x 5 ml (12009258)

Documents

Number Description Options
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
1929 Bio-Scale Ceramic Hydroxyapatite Type I Column Product Information Sheet, Rev A Click to download
6484 High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
6749 Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes, Ver A Click to download [ Add to Cart (Free) ]

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