
Overview
Specifications | |
Sizes | 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes |
Recommended flow rate | Varies for each support type* |
Maximum operating pressure | 1, 5, and 10 ml: 45 psi 50 ml: 40 psi |
Fittings | Female luer inlet and male luer outlet |
Column material | Polypropylene |
Frit material | Polyethylene (HDPE) |
Dimensions | 1 ml — 40 mm length x 5.6 mm inner diameter 5 ml — 40 mm length x 12.6 mm inner diameter 10 ml — 80 mm length x 12.6 mm inner diameter** 50 ml — 80 mm length x 28.2 mm inner diameter** |
* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.
Cartridge by Application | Support | Type of Separation | Chemical Form | Protein Capacity/ml* | Catalog Numbers by Cartridge Size |
||
5 x 1 ml | 1 x 5 ml | 5 x 5 ml | |||||
Protein and Plasmid Purification | |||||||
High Q | Macro-Prep High Q | Strong anion exchange | –N+(CH3)3 | ≥37 mg BSA | 7324120 | 7324122 | 7324124 |
UNOsphere Q | UNOsphere Q | Strong anion exchange | –N+(CH3)3 | 180 mg BSA | 7324100 | 7324102 | 7324104 |
UNOsphere S | UNOsphere S | Strong cation exchange | –SO3- | 60 mg human lgG | 7324110 | 7324112 | 7324114 |
DEAE | Macro-Prep DEAE | Weak anion exchange | –HN+(C2H5)2 | >35 mg protein | 7324140 | 7324142 | 7324144 |
Protein Purification | |||||||
High S | Macro-Prep High S | Strong cation exchange | –SO3- | ≥49 mg human lgG | 7324130 | 7324132 | 7324134 |
IgG Purification | |||||||
DEAE Blue | DEAE Affi-Gel Blue | Affinity |
Cibacron Blue F3GA and DEAE |
0.2–1.0 ml serum |
— | 7324632 | 7324634 |
Antibody Purification | |||||||
Protein A | UNOsphere SUPrA | Affinity | Recombinant protein A | 30 mg/ml human IgG | 7324200, 1 x 1 ml | 7324201, 5 x 1 ml | 7324202, 1 x 5 ml |
Protein A | Affi-Prep Protein A | Affinity | Protein A | 8–10 mg mouse monoclonal lgG1; 16–23 mg human lgG |
7324600 | 7324602 | — |
CHT Type I | CHT Ceramic Hydroxyapatite Type I, 40 µm |
Hydroxyapatite | (Ca5(PO4)3OH)2 | 25–60 mg human lgG | — | 7324322 | 7324324 |
CHT Type II | CHT Ceramic Hydroxyapatite Type II, 40 µm |
Hydroxyapatite | (Ca5(PO4)3OH)2 | 15–25 mg human lgG | — | 7324332 | 7324334 |
Affinity-Tagged Protein Purification | |||||||
IMAC** | Nuvia IMAC | Affinity | Nitrilotriacetic acid | ≥40 mg recombinant histidine-tagged protein |
— | 7800811 | 7800812 |
IMAC** | Profinity IMAC | Affinity | Iminodiacetic acid | ≥15 mg recombinant Histidine-tagged protein |
7324610 | 7324612 | 7324614 |
GST** | Profinity GST | Affinity | Glutathione | ≥10 mg/ml recombinant GST-tagged protein |
7324620 | 7324622 | 7324624 |
Purification of Serum Proteins, Enzymes | |||||||
Blue | Affi-Gel Blue | Affinity | Cibacron Blue F3GA |
0.2 ml serum 11 mg albumin |
— | 7324642 | 7324644 |
Cartridge by Application | Support | Type of Separation | Chemical Form | Protein Capacity/ml* | Catalog Numbers by Cartridge Size | ||||
1 x 5 ml |
5 x 5 ml |
5 x 10 ml |
1 x 50 ml |
5 x 50 ml |
|||||
Desalting, Buffer Exchange | |||||||||
P-6 | Bio-Gel P-6 | Gel filtration | Polyacrylamide | 10–22% CV*** | 7324502 | 7324504 | 7325304 | 7325312 | 7325314 |
* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.
Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Workflow for mAb purification with UNOsphere SUPrA affinity, UNOsphere Q, and CHT Type 1 media.
Type I | Type II | |
Functional groups | Ca2+, PO43-, F- | Ca2+, PO43-, F- |
Observed dynamic binding capacity bovine lgG | 14–17 mg/g CFT | 30–32 mg/g CFT |
Observed dynamic binding capacity lysozyme (Lys) | ≥25 mg/g CFT | 17–21 mg/g CFT |
Nominal pore diameter | 600–800 A | 600–800 A |
Maximum backpressure | 55 bar (800 psi) | 55 bar (800 psi) |
Nominal mean particle size | 40 ± 4 µm | 40 ± 4 µm |
Types I and II | |
Typical linear flow rate range | 50–300 cm/hr |
pH Stability | 5–14 pH |
Base Stability | |
Regeneration | Normal — 400 mM sodium phosphate, pH6.8 Difficult — 400 mM trisodium phosphate, pH 11–12 Basic proteins (pl >7)– 1–2 M NaCl or KCI,pH 10–12 |
Sanitation | Perform CIP, then 3–5 CV 1–2 N NaOH or KOH; exposure time ≥1 hr |
Recommended column storage | 0.1–1 N NaOH or KOH |
Shelf life (dry, unused material) | >2 years |
* 40 µm particles, 300 cm/hr, 5 mM sodium phosphate, pH 6.5.
** 40 µm particles, 300 cm/hr, 5 mM sodium phosphate, pH 6.5.
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