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      Bio-Scale™ Mini Affi-Gel® Blue Cartridges

      lsr_bio-scale_mini_cartridges.jpg

      Overview

      Bio-Scale Mini Affi-Gel Blue Cartridges are 5 ml cartridges filled with 50–100 mesh Affi-Gel Blue Support for use in affinity chromatography. It is ideally suited for albumin removal.
      Read full description Ordering Information

      Description

      Description

      Bio-Scale Mini Affi-Gel Blue cartridges are 5 ml Bio-Scale Mini cartridges filled with 100–200 mesh Affi-Gel Blue support for use in affinity chromatography. This form of Affi-Gel Blue gel is a crosslinked agarose bead with covalently attached cibacron blue F3GA dye that is ideally suited for albumin removal. Proteins and peptides are bound and released with a high degree of specificity by manipulating the composition of eluent buffers.

      Bio-Scale Mini cartridges have a patented* double-wall cartridge design that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts.

      Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems. Bio-Scale Mini affinity cartridges can also be run on the Profinia protein purification system.

      * U.S. patent 7,208,087.

      Specifications

      Specifications

      Size
      5 ml bed volume
      Dimensions (L x ID)* 
      40 x 12.6 mm
      Maximum pressure tolerance
      10 psi (680 mbar, 0.26mPa)**
      Operational flow rates
      0.5–2.0 ml/min (25–100 cm/hr)
      Fittings
      Female luer fitting inlet; male luer fitting outlet
      Column material
      Polypropylene
      Frit material
      Polyethylene (HDPE)
      Shipping conditional
      0.02 M sodium phosphate, pH 7.4, 0.05% NaN3
      Storage recommendations
      0.02 M sodium phosphate, pH 7.4, 0.05% NaN3
      Autoclavability
      Not autoclavable
      * L, length; ID, inner diameter.
       
      ** Although the pressure limitation of the cartridge is 45 psi, the agarose gel tends to compress above 10 psi (generally 3.0 ml/min).
       

      Ordering

      Ordering

      items

      Use the filters below to refine results!

      732-4642_tn.jpg
      (Discontinued) Bio-Scale Mini Affi-Gel Blue Cartridge
      7324642
      Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
      The following replacement is recommended:
      • EconoFit Affi-Gel Blue Column, 1 x 5 ml ( 12009234 )
      732-4644_tn.jpg
      (Discontinued) Bio-Scale Mini Affi-Gel Blue Cartridges
      7324644
      Pkg of 5, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
      The following replacement is recommended:
      • EconoFit Affi-Gel Blue Columns, 5 x 5 ml ( 12009235 )
      732-4620_tn.jpg
      (Discontinued) 1 ml Empty Bio-Scale Mini Cartridges
      7324660

      Pkg of 5, 1 ml, empty chromatography columns, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

      732-4624_tn.jpg
      (Discontinued) 5 ml Empty Bio-Scale™ Mini Cartridges
      7324661
      Pkg of 5, 5 ml, empty chromatography columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

      Documents

      Documents

      Number Description Options
      6498
      Laboratory-Scale Protein Purification — Column Selection Guide
      5584
      Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
      10006402
      Bio-Scale Mini Affi-Gel® Blue and DEAE Affi-Gel Blue Cartridges, 5 ml, Instruction Manual, Rev C