Bio-Scale™ Mini Affi-Gel® Blue Cartridges

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Bio-Scale Mini Affi-Gel Blue cartridges

Overview

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 7324120 7324122 7324124
UNOsphere Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 7324100 7324102 7324104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 7324110 7324112 7324114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 7324140 7324142 7324144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 7324130 7324132 7324134
IgG Purification
DEAE Blue DEAE Affi-Gel Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

7324632 7324634
Antibody Purification
Protein A UNOsphere SUPrA Affinity Recombinant protein A 30 mg/ml human IgG 7324200, 1 x 1 ml 7324201, 5 x 1 ml 7324202, 1 x 5 ml
Protein A Affi-Prep Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
7324600 7324602
CHT Type I CHT Ceramic
Hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 7324322 7324324
CHT Type II CHT Ceramic
Hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 7324332 7324334
Affinity-Tagged Protein Purification
IMAC** Nuvia IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
7800811 7800812
IMAC** Profinity IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
7324610 7324612 7324614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
7324620 7324622 7324624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
7324642 7324644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel P-6 Gel filtration Polyacrylamide 10–22% CV*** 7324502 7324504 7325304 7325312 7325314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Bio-Scale Mini Affi-Gel Blue Cartridges are 5 ml cartridges filled with 50–100 mesh Affi-Gel Blue Support for use in affinity chromatography. It is ideally suited for albumin removal.
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Ordering Information


Description

Bio-Scale Mini Affi-Gel Blue cartridges are 5 ml Bio-Scale Mini cartridges filled with 100–200 mesh Affi-Gel Blue support for use in affinity chromatography. This form of Affi-Gel Blue gel is a crosslinked agarose bead with covalently attached cibacron blue F3GA dye that is ideally suited for albumin removal. Proteins and peptides are bound and released with a high degree of specificity by manipulating the composition of eluent buffers.

Bio-Scale Mini cartridges have a patented* double-wall cartridge design that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts.

Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems. Bio-Scale Mini affinity cartridges can also be run on the Profinia protein purification system.

* U.S. patent 7,208,087.


Specifications

Size 5 ml bed volume
Dimensions (L x ID)*  40 x 12.6 mm
Maximum pressure tolerance 10 psi (680 mbar, 0.26mPa)**
Operational flow rates 0.5–2.0 ml/min (25–100 cm/hr)
Fittings Female luer fitting inlet; male luer fitting outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Shipping conditional 0.02 M sodium phosphate, pH 7.4, 0.05% NaN3
Storage recommendations 0.02 M sodium phosphate, pH 7.4, 0.05% NaN3
Autoclavability Not autoclavable
* L, length; ID, inner diameter.  
** Although the pressure limitation of the cartridge is 45 psi, the agarose gel tends to compress above 10 psi (generally 3.0 ml/min).  

Ordering

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Bio-Scale Mini Affi-Gel Blue Cartridge

7324642
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Affi-Gel Blue Column, 1 x 5 ml (12009234)
Bio-Scale Mini Affi-Gel Blue Cartridges

7324644
Pkg of 5, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Affi-Gel Blue Columns, 5 x 5 ml (12009235)
1 ml Empty Bio-Scale™ Mini Cartridges

7324660
Pkg of 5, 1 ml, empty chromatography columns, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.
5 ml Empty Bio-Scale™ Mini Cartridges

7324661
Pkg of 5, 5 ml, empty chromatography columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

Documents

Number Description Options
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
10006402 Bio-Scale Mini Affi-Gel® Blue and DEAE Affi-Gel Blue Cartridges, 5 ml, Instruction Manual, Rev C Click to download

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