Bio-Scale™ Mini Affi-Gel® Blue Cartridges

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Overview

Bio-Scale Mini Affi-Gel Blue Cartridges are 5 ml cartridges filled with 50–100 mesh Affi-Gel Blue Support for use in affinity chromatography. It is ideally suited for albumin removal.
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Description

Description

Bio-Scale Mini Affi-Gel Blue cartridges are 5 ml Bio-Scale Mini cartridges filled with 100–200 mesh Affi-Gel Blue support for use in affinity chromatography. This form of Affi-Gel Blue gel is a crosslinked agarose bead with covalently attached cibacron blue F3GA dye that is ideally suited for albumin removal. Proteins and peptides are bound and released with a high degree of specificity by manipulating the composition of eluent buffers.

Bio-Scale Mini cartridges have a patented* double-wall cartridge design that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts.

Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems. Bio-Scale Mini affinity cartridges can also be run on the Profinia protein purification system.

* U.S. patent 7,208,087.

Specifications

Specifications

Size
5 ml bed volume
Dimensions (L x ID)* 
40 x 12.6 mm
Maximum pressure tolerance
10 psi (680 mbar, 0.26mPa)**
Operational flow rates
0.5–2.0 ml/min (25–100 cm/hr)
Fittings
Female luer fitting inlet; male luer fitting outlet
Column material
Polypropylene
Frit material
Polyethylene (HDPE)
Shipping conditional
0.02 M sodium phosphate, pH 7.4, 0.05% NaN3
Storage recommendations
0.02 M sodium phosphate, pH 7.4, 0.05% NaN3
Autoclavability
Not autoclavable
* L, length; ID, inner diameter.
 
** Although the pressure limitation of the cartridge is 45 psi, the agarose gel tends to compress above 10 psi (generally 3.0 ml/min).
 

Ordering

Ordering

items

Use the filters below to refine results!

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7324642
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4644_tn.jpg
7324644
Pkg of 5, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
732-4620_tn.jpg
7324660

Pkg of 5, 1 ml, empty chromatography columns, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

732-4624_tn.jpg
7324661
Pkg of 5, 5 ml, empty chromatography columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system

Documents

Documents

Number Description Options
6498
Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C
[ Add to Cart (Free) ]
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
10006402
Bio-Scale Mini Affi-Gel® Blue and DEAE Affi-Gel Blue Cartridges, 5 ml, Instruction Manual, Rev C