Bio-Plex Pro™ Human Cancer Biomarker Assays

Image
lsr_bp_pro_human_cancer_biomarker_assays.jpg

Overview

Multiplex immunoassays built on magnetic beads for the quantitation of multiple cancer biomarkers. Available as premixed panels, singleplex sets, and custom configurations.
Read full description

Description

Description

Bio-Plex Pro Human Cancer Biomarker Assays are a unique blend of magnetic bead–based assays designed to meet the needs of the most discerning preclinical and clinical researchers. The multiplex format enables robust and reproducible measurement of 34 cancer biomarkers involved in disease processes such as:

  • Angiogenesis
  • Metastasis
  • Cell proliferation
  • Cell adhesion/migration
  • Apoptosis
  • Inflammation

Bio-Plex Pro Human Cancer Biomarker Assays are available in two panels.

Panel 1 consists of receptors, cytokines, chemokines, growth factors, and hormones and is available as a premixed 16-plex all-in-one kit.

Panel 2 consists of ligands for panel 1, cytokines, chemokines, and growth factors and is available as a premixed 18-plex all-in-one kit.

Both panels are available in customizable analyte configurations and singleplex sets.

Panel 1

  • sEGFR
  • FGF-basic
  • Follistatin
  • G-CSF
  • sHER-2/neu
  • HGF
  • sIL-6Rα
  • Leptin
  • Osteopontin
  • PDGF-AB/BB
  • PECAM-1
  • Prolactin
  • SCF
  • sTIE-2
  • sVEGFR-1
  • sVEGFR-2

Panel 2

  • Angiopoietin-2
  • sCD40L
  • EGF
  • Endoglin
  • sFASL
  • HB-EGF
  • IGFBP-1
  • IL-6
  • IL-8
  • IL-18*
  • PAI-1
  • PLGF
  • TGF-α
  • TNF-α
  • uPA
  • VEGF-A
  • VEGF-C
  • VEGF-D

* IL-18 is not available in singleplex format.

Based on 6.5 µm magnetic beads, these assay panels are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic beads for simplified plate processing
  • All-in-one, space-saving complete kits
  • Premixed standards for generation of curves over a broad dynamic range for each target
  • Reagent kit II for reliable performance in serum and plasma samples
  • Optimized protocol for high precision and broad assay working ranges
  • Assay quick guide to get you started right away
  • 2-level quality controls included with premixed kits

Other Assay Formats Available

Instrument Compatibility

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Related Products

Bio-Plex Pro™ Human Inflammation Assays
For the first time, the key biomarkers of inflammation from the TNF superfamily proteins, IFN family proteins, Treg cytokines, and MMPs can be measured using a single multiplex kit. Bio-Plex Pro Human Inflammation Assays are available in larger 24-plex and 37-plex screening panels and a pathway-focused Treg 12-plex panel, along with singleplex and custom-configured assays.

Accessories

Accessories

171-304500_tn.jpg
171304500
1.5 L, wash buffer for use with Bio-Plex Pro wash stations; not for use with phosphoprotein assays
171-020100_tn.jpg
171020100
Includes magnetic washer and adjustment hex tools for use in manual wash steps for all Bio-Plex Magnetic Assays
171-025001b_tn.jpg
171025001
Pkg of 40, 96-well plate, for use with Bio-Plex Pro wash stations when using magnetic bead–based assays
300-34376_tn.jpg
30034376
Microplate wash station for magnetic bead–based assays, includes magnetic plate carrier, waste bottle, 2 buffer bottles
171-304502_tn.jpg
(Discontinued) Filter Plate
171304502
Pkg of 1, 96-well plate, with clear plastic lid and tray, for Bio-Plex Assays using the vacuum wash method, sealing tape not included

Kits & Reagents

Kits & Reagents

171-304070_tn.jpg
171304055
1 x 96-well, includes detection antibody diluent, standard diluent HB, sample diluent HB, assay and wash buffers, streptavidin-PE, filter plate, and sealing tape, for use with vacuum separation methods
171304055M
1 x 96-well, includes detection antibody diluent, standard diluent HB, sample diluent HB, assay and wash buffers, streptavidin-PE, flat bottom plate, and sealing tape, for use with magnetic separation methods
171-DC5000_tn.jpg
171DC5001
Pkg of 50 lot-matched vials, lyophilized mixture of 16 standard antigens
Bio-Plex-Pro-Human-Cancer-Biomarker-Panel-2-18-plex Standards-SKU-171-DC6001.jpg
171DC6001
Pkg of 50 lot-matched vials, lyophilized mixture of 18 standard analytes

Documents

Documents

Number Description Options
6532
Cross-Reactivity of Bio-Plex Pro Human Cancer Biomarker Assays to Mouse Proteins, Rev A
6335
Bio-Plex Multiplex Immunoassays Analyte Guide
6304
Development and Validation of Bio-Plex Pro Human Cancer Biomarker Panel 2, Rev A
6156
Composite Profiling of Angiogenic Factors Using Bio-Plex Pro Human Cancer Biomarker Panel 1, Rev A
5405
Bio-Plex® Multiplex System Brochure, Ver E
[ Add to Cart (Free) ]
10000080121
Bio-Plex Pro™ Human Th17 Cytokine Assays Quick Guide
10022186
Instruction Manual, Bio-Plex Pro Human Cancer Biomarker Assays, Rev B
6457
The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B
6458
Bio-Plex Success Guide/Assay Sample Prep, Rev B
7793
Cancer Vaccines: Getting Ready for a Decade of Big Breakthroughs