Aurum™ Total RNA 96 Kit

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Aurum Total RNA 96 Kit

Overview

RNA Isolation Selection Guide
  Aurum™ Total RNA Kits PureZOL™ RNA Isolation Reagent
Mini Kit Fatty and Fibrous Tissue Kit 96 Kit
Format Mini column Filtration (vacuum or spin) Mini column Filtration (vacuum or spin) 96-well plate Filtration (vacuum or spin)
Maximum starting amounts        
   Cultured cells 2 x 106 1 x 106 1 x 107
   Bacterial cells 2.4 x 109 8 x 108 2.4 x 109
   Yeast cells 3 x 107 2 x 107 3 x 107
   Animal tissue — hard 20 mg 100 mg 100 mg
   Animal tissue — soft    to moderately hard 40 mg 100 mg 100 mg
   Plant tissue 40 mg 100 mg 100 mg
Isolation method Silica membrane Lysis with PureZOL Reagent, purification on silica membrane Silica membrane Organic extraction
Number of preps 50 mini preps 50 mini preps 2 x 96-well plate 50 or 100 preps (1 ml/prep)
Number of washes 3 3 3
DNase I included* Yes Yes Yes No
DNase I digest time 15 min (animal tissue, 25 min) 15 min 10 min
Total preparation time <50 min (1.5 hr with DNase I) <50 min (1.5 hr with DNase I) <60 min <60 min
Binding capacity >100 µg >100 µg >40 µg
Elution volume 2 x 40 µl 2 x 40 µl 80 µl 30–100 µl
Yield** 28 µg/2 x 106 NIH 3T3 cells 96 µg/100 mg brain 10 µg/1 x 106 NIH 3T3 cells 1.15 µg/mg brain
  38 µg/2 x 106 HeLa cells 58 µg/100 mg breast 17 µg/1 x 106 HeLa cells 1.37 µg/mg fat
  20 µg/40 mg brain 85 µg/100 mg heart 11 µg/2 x 107 S. cerevisiae cells 1.2 µg/mg heart
  52 µg/20 mg thymus 63 µg/100 mg skin 5 µg/8 x 108 E. coli cells 0.66 µg/mg cartilage
  132 µg/40 mg spleen 54 µg/100 mg cartilage 5 µg/8 x 108 B. cereus cells 1.2 µg/mg skin
  36 µg/40 mg lung     1.0 µg/mg potato
  32 µg/40 mg potato     1.37 µg/1 x 105 293H cells
  28 µg/3 x 107 S. cerevisiae cells     0.1 µg/mg Arabidopsis
  54 µg/2.4 x 109 E. coli cells     0.38 µg/mg A. niger
        0.63 µg/2.4 x 107 E. coli cells
        0.31 µg/3 x 105 S. cerevisiae cells
Purity A260/A280 of 1.9–2.1 A260/A280 of 1.9–2.1 A260/A280 of 1.9–2.1 A260/A280 of 1.9–2.1
 

* Removal not required.

** Yields will vary depending on the development stage, tissue type or cell line, and growth conditions used.

Isolated RNA Quality

Automated electrophoresis of high-quality eukaryotic total RNA. Total RNA was isolated from a homogeneous culture of 1 x 106 NIH 3T3 cells using the Aurum total RNA 96 kit. Start times of the 18S and 28S ribosomal RNAs were 40.0 and 45.6 sec, respectively. The clearly defined peaks and minimal background fluorescence indicate intact, undegraded RNA.

Isolated RNA Performance via qPCR


Real-time RT-PCR with total RNA isolated using the Aurum total RNA 96 kit. Total RNA isolated from a culture of 1 x 106 HeLa cells was used in two-step real-time RT-PCR. Reverse transcription reactions were performed on tenfold dilutions (1 µg to 10 pg) of the HeLa total RNA using the iScript cDNA synthesis kit. cDNA from these reactions was used to detect human α-tubulin gene expression levels using gene-specific primers and iQ SYBR® Green supermix. Real-time data were acquired on Bio-Rad's iCycler iQ system. A, real-time curves for detection of human α-tubulin cDNA; B, standard curve showing r = 0.998, efficiency = 97.2%. RFU, relative fluorescence units.

The Aurum total RNA 96 kit isolates high-quality DNA-free total RNA from cultured cells, bacteria, and yeast in a 96-well format for high-throughput total RNA isolation.
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Description

The Aurum total RNA 96 kit isolates high-quality DNA-free total RNA from cultured cells, bacteria, and yeast in a 96-well format for high-throughput total RNA isolation. The kit utilizes a stringent reagent composed of guanidine isothiocyanate and β-mercaptoethanol for efficient sample lysis and quick RNase inactivation, followed by purification on silica membrane in a 96-well plate format using a spin- or vacuum-mediated protocol. The entire purification process, including DNase I digest, is completed in less than 60 minutes. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications.

Features include:

  • High-throughput total RNA isolation in less than 60 minutes
  • RNase-free reagents and plasticware, including DNase I for removal of genomic DNA at no additional cost
  • Rapid silica binding-based purification technology in a 96-well plate format
  • Easy-to-use spin- or vacuum-compatible protocol

Specifications

Format 96-well plate
Method Spin- or vacuum-mediated silica binding
Number of preps 192 (2 x 96-well plate)
Maximum starting material amounts 1 x 106 cultured cells
8 x 108 bacterial cells
2 x 107 yeast cells
Binding Capacity >40 µg purified total RNA
Purity A260/A280 of 1.9–2.1
RNA size distribution >200 nucleotides
Preparation time <60 min (including DNase I digest)
Maximum elution volume 80 µl
Minimum elution volume 30 µl*
10 µg/1 x 106 NIH 3T3 cells
17 µg/1 x 109 HeLa cells
11 µg/2 x 107 S. cerevisiae cells
Yield** 5 µg/8 x 108 E. Coli cells
5 µg/8 x 108 B. cereus cells
*The elution volume is reduced to 30µl when
isolating total RNA from small amounts of
starting material (<500,000 cells or 10 mg of tissue).
 
**Yields will vary depending on the
developmental stage, tissue type or cell line,
and growth conditions used.
 

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Aurum™ Total RNA 96 Kit

7326800
DNA-free RNA isolation kit, includes RNA binding plates, collection microplates, lyophilized DNase I, RNase-free reagents, sealing tape, for 2 x 96-well preps

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$676.00 
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Accessories

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DNase I

7326828
Pkg of 1 vial, refill reagent for Aurum total RNA kits, RNase-free, lyophilized

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$66.00 
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Aurum DNase Dilution Solution

7326805
20 ml, refill reagent for Aurum total RNA kits

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$16.00 
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Aurum Total RNA Wash Low-Stringency Solution

7326804
60 ml, refill reagent for Aurum total RNA kits

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$35.00 
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Aurum Total RNA Wash High-Stringency Solution

7326803
150 ml, refill reagent for Aurum total RNA kits, RNase-free

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$45.00 
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Aurum Total RNA Lysis Solution

7326802
85 ml, refill reagent for Aurum total RNA kits, RNase-free

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$61.00 
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Aurum Total RNA Elution Solution

7326801
20 ml, refill reagent for Aurum total RNA kits, RNase-free

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$16.00 
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Documents

Number Description Options
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
2925 Aurum Total RNA 96 Kit Protocol Overview Click to download
4110131 Instruction Manual, Aurum™ Total RNA 96 Kit, Rev C Click to download
7331 Protocol for Viral RNA Extraction using Aurum Total RNA Kits, Ver A Click to download
6763 Gene Expression Real-Time PCR Workflow Flier Click to download

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