1-Step Quantitative Reverse Transcription PCR (RT-qPCR) from RNA

Print
1-Step Quantitative Reverse Transcription PCR (RT-qPCR) from RNA

Overview

Superior Dynamic Range and Sensitivity Performance of iTaq Universal SYBR® Green One-Step Kit

A cell lysate and a 226 bp human GAPDH assay were used to determine dynamic range and sensitivity performance of different kits on a CFX384 real-time PCR instrument.

A. The iTaq Universal SYBR® Green One-Step Kit

B. The Qiagen QuantiTect SYBR® Green FT-PCR kit

C. Life Technologies Power SYBR RNA-to-CT kit

iTaq Universal SYBR® Green One-Step Kit performed better than the other kits tested in terms of cell input, PCR efficiency and R2


  iTaq Universal SYBR® Green One-Step Kit (Bio-Rad) QuantiTect SYBRGreen RT-PCR Kit (Qiagen) Power SYBRRNA-to-Ct Kit (Life Technologies)
Cell Input 1,000 to 0.001 1,000 to 1 100 to 0.1
PCR Efficiency 96.8% 94.5% 83.3%
R2 0.997 0.977 0.981
Greater Sensitivity with iTaq Universal Probes One-Step Kit

Bio-Rad’s iTaq Universal Probes One-Step Kit (A, C) and TaqMan RNA-to-CT 1-step kit from Life Technologies (B, D) were used in high-performing duplex and singleplex RT-qPCR reactions. Input RNA was 10–100 ng. Reactions were performed on a CFX96 real-time PCR detection system.

The iTaq Kit exhibits a slight 2–quantification cycle (Cq) delay from singleplex to duplex, which is typical for duplex reactions; however, the TaqMan kit exhibits a 4-Cq delay (greater than one log of dynamic range), which indicates less sensitivity.

Duplex PGK1, PPIA Assay

iTaq universal probes one-step kit
iTaq Universal Probes One-Step Kit
TaqMan RNA-to-CT 1-step kit (Life Technologies)
TaqMan RNA-to-CT 1-step kit (Life Technologies)

Singleplex PGK1 Assay

iTaq universal probes one-step kit
iTaq Universal Probes One-Step Kit
TaqMan RNA-to-CT 1-step kit (Life Technologies)
TaqMan RNA-to-CT 1-step kit (Life Technologies)
Superior Dynamic Range, Sensitivity, and Linearity with GC-Rich Template

Superior dynamic range and sensitivity performance of iTaq Universal SYBR® Green One-Step Kit.

A 93-bp humanBAX assay was performed with 500 ng to 5 pg of total RNA on an AB ViiA 7 real-time PCR instrument.

The delayed cycle threshold (Ct) values, loss of dynamic range and the 500 ng input, and compression of the 50 ng input in the Power SYBR® RNA-to-Ct kit demonstrated RT-qPCR inhibition at higher RNA inputs and lack of low-level target expression detection. ΔRn, baseline-corrected normalized reporter.

iTaq Universal SYBR<sup>®</sup> Green one-step kit PCR efficiency of 102.4% and R2 of 0.999 Life Technologies Power SYBR<sup></sup> RNA-to-Ct kit PCR efficiency of 111.8% and R2 of 0.992
iTaq Universal SYBR® Green One-Step Kit PCR efficiency of 102.4% and R2 of 0.999 Life Technologies Power SYBR® RNA-to-Ct kit PCR efficiency of 111.8% and R2 of 0.992

Optimal RT-qPCR efficiency and sensitivity with iTaq Universal Probes One-Step Kit.

A 72% GC-rich CBP assay with an input of 100 ng–10 pg RNA was performed on a CFX96 real-time PCR detection system.

Brilliant III ultra-fast QRT-PCR master mix (Agilent Technologies) produced quantification cycle (Cq) values with less than optimal data precision for the technical replicates.

iTaq Universal SYBR<sup>®</sup> Green one-step kit PCR efficiency of 98.1% and R2 of 0.999 Brilliant III ultra-fast QRT-PCR master mix (Agilent Technologies) PCR efficiency of 87.3% and R2 ≥0.984
iTaq Universal SYBR® Green One-Step Kit PCR efficiency of 98.1% and R2 of 0.999 Brilliant III ultra-fast QRT-PCR master mix (Agilent Technologies) PCR efficiency of 87.3% and R2 ≥0.984

 

Inhibitor Tolerance and Multiplexing

Superior RT-qPCR performance in a triplex reaction with iTaq Universal Probes One-Step Kit.

An XPO (FAM), TUB (HEX), and GAPDH (Quasar) assay set with an input of 100 ng–100 fg RNA was performed on a CFX96 Real-Time PCR Detection System using the iTaq Kit and two competitor products.

 
Dye Logs Standard Curve, R2 Efficiency, %
 FAM 5 0.998 106
 HEX 4 0.999 101
 Quasar 6 0.99 103

The iTaq Universal Probes One-Step Kit (A) exhibited the greatest dynamic range across all three assays, which indicates a high degree of sensitivity. In addition, the iTaq Universal Probes One-Step Kit demonstrated the lowest standard deviations for technical replicates.

Dye Logs Standard Curve, R2 Efficiency, %
 FAM 4 0.998 101
 HEX 4 0.993 97.6
 Quasar 4 0.998 97.5

Brilliant III ultra-fast QRT-PCR master mix from Agilent Technologies (B) and TaqMan RNA-to-CT 1-step kit from Life Technologies (C) did not perform as well, exhibiting four logs of dynamic range for each assay (lower sensitivity) and higher standard deviations for technical replicates (lower data precision).

Dye Logs Standard Curve, R2 Efficiency, %
 FAM 4 0.999 96.7
 HEX 4 0.999 96.7
 Quasar 4 0.999 95.6
 

Great ethanol tolerance of iTaq Universal Probes One-Step Kit

Final concentration 10 pg 200 pg
Average Cq dCq Average Cq dCq
0% 27.4 - 23.0 -
0.25% 27.3 -0.1 22.9 -0.1
1.25% 27.8 0.4 23.4 0.4

Singleplex reactions were prepared using iTaq Universal Probes One-Step Kit with two RNA inputs of 10 pg and 200 pg and ethanol was spiked in at a final concentration of 0%, 0.25, and 1.25% to evaluation the tolerance of the kit. The iTaq Universal Probes One-Step Kit resulted in less than 0.5 dCq between reactions indicating minimal inhibition by ethanol.


Superior RT-qPCR inhibitor tolerance of iTaq Universal SYBR® Green One-Step Kit.

A human ACTB assay was performed with 200 ng of total RNA, and chocolate and heparin were spiked in to evaluate the tolerance of the iTaq Universal SYBR® Green One-Step Kit () versus the Life Technologies Power SYBR RNA-to-Ct () and Qiagen QuantiTect SYBR® Green RT-PCR kit ().        

A, Chocolate spiked in from 0.01 to 0.2% resulted in a greater quantification cycle (Cq) delay from the control sample with the Life Technologies Power SYBR RNA-to-Ct kit (), indicating less tolerance for RT-qPCR inhibitors than the iTaq Universal SYBR® Green One-Step Kit ().

B. Heparin spiked in from 200 to 3,200 pg/µl caused the 17.83 Cq delay at 200 pg/µl and eventual complete inhibition with the Life Technologies Power SYBR RNA-to-Ct kit () but only minor Cq delays with the iTaq Universal SYBR® Green One-Step Kit ()

C. Heparin spiked in from 200 to 1,600 pg/µl produced a greater Cq delay from the control (unspiked) sample with the Qiagen QuantiTect SYBR® Green RT-PCR kit () than the iTaq Universal SYBR® Green One-Step Kit ().

Superior Data Precision and Reproducibility

iTaq Universal Probes One-Step Kit

Superior data precision and reproducibility with iTaq universal probes one-step kit

GAPDH assay with input of 8.78 to 100 ng of RNA shows high precision and reproducibility of results. PCR was performed on a CFX96 Real-Time Detection System (RFU, relative fluorescence units.)

iTaq Universal SYBR Green One-Step Kit: Superior precision compared to other kits.

A human TUBA assay with an input of 8.78 to 100 ng of total RNA was performed on an ABI 7900 real-time instrument using different RT-PCR kits (ΔRn, baseline-corrected normalized reporter.)

The iTaq universal SYBR<sup>®</sup> Green one-step kit demonstrated robust data acquisition even with small-fold changes.

A. The iTaq Universal SYBR® Green One-Step Kit

 
The Qiagen QuantiTect SYBR<sup>®</sup> Green RT-PCR kit and Life Technologies Power SYBR precision RNA-to-Ct kit

B. The Qiagen QuantiTect SYBR® Green RT-PCR kit

 
Both showed less than optimal precision. ΔRn, baseline-corrected normalized reporter.

C. Life Technologies' Power SYBR® precision RNA-to-Ct kit

Scroll upScroll down


CFX Loyalty Program

Earn a free one-year service contract on your CFX System with purchase of Reagents.

CFX Loyalty Program

iTaq Universal One-Step RT-qPCR Kits are a fast and convenient solution for real-time PCR using the powerful combination of iScript RNase H+ MMLV reverse transcriptase and hot-start iTaq DNA Polymerase in one reaction, providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity, and inhibitor tolerance without affecting performance.

Available for SYBR®Green and probe-based qPCR assays, iTaq Universal One-Step Kits are ideal for low-to mid-throughput real-time PCR screening and validation. A simplified work flow and reduced cycling times enable screening and validation of the greatest number of samples and targets in the shortest period of time.

Features and Benefits of iTaq Universal One-Step RT-qPCR Kits

  • Enhanced efficiency, specificity, and sensitivity— our patented inhibitor reducer* prevents interference of the DNA polymerase by the RT enzyme during cDNA replication
  • Compatible with all real-time PCR instruments — our universal reference dye enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system
  • Superior gene expression results under various cycling conditions — our robust formulation delivers consistent performance in standard or fast cycling conditions, primer concentrations, sample and target types, and temperature ranges, allowing cost and time savings
  • Obtain superior results with singleplex and multiplex reactions — advanced formulation enables the simultaneous amplification of up to two targets, resulting in higher data precision with fewer pipet steps and reduced sample usage

Instrument Compatibility

iTaq Universal One-Step RT-qPCR Kits are compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
BioFire
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses of iTaq Universal One-Step RT-qPCR Kits

  • Gene expression analysis
  • Multiplexing
  • Absolute quantification
  • Mutation detection
  • Pathogen detection
  • Viral detection
  • Genetically modified organisms (GMO) characterization
  • Genetic profiling

Related Categories

Supporting Documentation

Online Resources

* U.S. patent 8,338,094.

Product format Two-tube system
Kit content iScript reverse transcriptase + 2x iTaq PCR reaction mix
Detection chemistry SYBR Green or probes
Multiplex capability (for probes only) Duplex (triplex possible depending on the abundance of the targets)
Inhibitor tolerance Tested with ethanol. Also compatible with up to 4 µl of crude lysates
5’ to 3’ exonuclease activity of the enzyme(s) Yes
Hot-start, activation time Yes, 1–3 min
Sample input 100 fg to 100 ng
Sensitivity 10 copies in a duplex reaction
Reference dye Patented universal dye preblended in the iTaq reaction mix
Instrument compatibility Both ROX-dependent (high- and low-) and -independent instruments
Product expiration (after shipping date) 12 months
RT storage at –20°C (after shipping date) 12 months
Reaction mix storage at 4°C (after thawing) Up to 3 months
Customer guarantee (minimum usage time) at –20°C 12 months
x

Account price failed to display. Please try reloading the page.Reload Page

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
email to

iTaq™ Universal SYBR® Green One-Step Kit, 100 x 20 µl rxns, 1 ml

1725150
100 x 20 µl reactions, 1 ml (1 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR® Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water

1725150 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5150_1371756447.xml 1725150 06/20/13 12:21 PM en_US true internet/product_SKU 2019-05-04 16:19:18 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5150_1371756447.xml /default/main/bio-rad/WORKAREA/default jcrucyr0 2017-11-14 07:07:36 true jcrucyr0 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5150_1371756447.xml LSR N /default/main/bio-rad en 06/20/23 12:25 AM 1555974592199 2019-05-04 16:19:18 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5150_1371756447.xml 1725150-itaq-universal-sybr-green-one-step-kit-100-x-20-ul-rxns-1-ml 1 1725150 iTaq Universal SYBR Green One-Step Kit iTaq™ Universal SYBR<sup>®</sup> Green One-Step Kit, 100 x 20 µl rxns, 1 ml 100 x 20 &micro;l reactions, 1 ml (1 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR<sup>&reg;</sup> Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript&trade; reverse transcriptase; and nuclease-free water 100 x 20 &micro;l reactions, 1 ml (1 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR<sup>&reg;</sup> Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript&trade; reverse transcriptase; and nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/itaq-universal-sybr-green-one-step-kit-1ml-172-5150-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/itaq-universal-sybr-green-one-step-kit-1ml-172-5150-view.jpg ,VN Molly Gregg iTaq Universal SYBR Green One-Step Kit 1 ml, one-step RT-PCR kit, includes 2x one-step SYBR Green reaction mix (Taq polymerase, buffers, ROX normalization dyes), iScript reverse transcriptase, and nuclease-free water, for 100 x 20 µl reactions 1725150, itaq, universal, sybr, green, one-step, one, step, rt-pcr, reverse, transcription, real-time, real, time, pcr, qpcr, detection, amplification, gene, expression 06/20/13 12:21 PM 06/20/23 12:25 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Our Universal Real-Time PCR Reagents Web App brings you in-depth product information, individual and competitive performance data, interactive tutorials, 3-D animated videos, technical manuals, and more.</p> <p>Use our combined iScript&trade; RNase H+reverse transcriptase and antibody hot-start iTaq DNA polymerase kit formulation to complete your SYBR<sup>&reg;</sup> Green real-time reactions in one step &ndash; PCR efficiency is improved over a wide dynamic range and under various conditions.</p> <ul> <li>Advanced formulation for amplifying difficult targets and challenging samples</li> <li>Can be combined with our <a href="/evportal/destination/commerce/product_detail?catID=M0HROA15">PrimePCR&trade; assays</a> for a simplified workflow</li> <li>Universal &mdash; works on any real-time PCR system</li> </ul> <h2 class="descHeader">Kit Components</h2> <ul> <li>Reverse transcriptase &ndash; 25 &micro;l</li> <li>Reaction mix &ndash; 1 ml (1 x 1 ml)</li> </ul> <h2 class="descHeader">Instrument Compatibility</h2> <p>iTaq Universal SYBR Green One-Step RT-qPCR Kit is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0*</li> </ul> </td> </tr> <tr> <td valign="top">BioFire</td> <td valign="top"> <ul> <li>LightScanner HR-1</li> <li>LightScanner 32*</li> </ul> </td> </tr> </tbody> </table> <p class="caption caption-margin">* BSA must be added according to instrument specifications</p> <h2 class="descHeader">Applications and Uses of the iTaq<sup>&reg;</sup> Universal SYBR<sup>&reg;</sup> Green One-Step Kit</h2> <ul> <li>qPCR/real-time PCR</li> <li>Gene expression analysis</li> <li>Absolute quantification</li> <li>Mutation detection</li> <li>Pathogen detection</li> <li>Viral detection (load)</li> <li>Genetically modified organism (GMO) characterization</li> <li>Genetic profiling</li> </ul> <h2 class="descHeader">Packaging Options</h2> <p>The <strong>iTaq&trade; universal SYBR<sup>&reg;</sup> Green one-step kit</strong> is also available in a format of <a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5151">500 reactions (171-5151)</a>.</p> <h2 class="descHeader">Related Products</h2> <p>Bio-Rad has also developed one-step kits for <a href="/en-us/product/itaq-universal-probes-one-step-kit">probe-specific applications</a>.</p> /webroot/web/html/lsr/products/SKUs 172-5150.html /webroot/web/html/lsr/products/SKUs/172-5150.html Product expiration (after shipping date) 2026 12 months 1 0 RT storage at –20°C (after shipping date) 2898 12 months 1 0 Reaction mix storage at 4°C (after thawing) 2896 Up to 3 months 1 0 Customer guarantee (minimum usage time) at –20°C 2530 12 months 1 0 false $229.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725150&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725150&CartQuote=QT&quantity= 1468 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725150&AddToDefaultWishList=true&quantity= 41106300 229.00 Product detail page 1725150
List Price: 
 
$229.00 
LoadingLoading...
EDU Price:
  What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
 
iTaq™ Universal SYBR® Green One-Step Kit, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725151
500 x 20 µl reactions, 5 ml (5 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR® Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water

1725151 templatedata/internet/product_SKU/data/ja_JP/LSR/Amplification---PCR/PCR-Reagents/172-5151_1371756630.xml 1725151 06/20/13 12:21 PM en_US true internet/product_SKU 2019-05-04 16:19:22 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5151_1371756630.xml /default/main/bio-rad/WORKAREA/default jcrucyt7 2017-11-14 07:07:36 true jcrucyt7 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5151_1371756630.xml LSR N /default/main/bio-rad en 06/20/23 12:30 AM 1555974592244 2019-05-04 16:19:22 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5151_1371756630.xml 1725151-itaq-universal-sybr-green-one-step-kit-500-x-20-ul-rxns-5-ml-5-x-1-ml 1 1725151 iTaq Universal SYBR Green One-Step Kit iTaq™ Universal SYBR<sup>®</sup> Green One-Step Kit, 500 x 20 µl rxns, 5 ml (5 x 1 ml) 500 x 20 &micro;l reactions, 5 ml (5 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR<sup>&reg;</sup> Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript&trade; reverse transcriptase; and nuclease-free water 500 x 20 &micro;l reactions, 5 ml (5 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR<sup>&reg;</sup> Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript&trade; reverse transcriptase; and nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/itaq-universal-sybr-green-one-step-kit-5ml-172-5151-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/itaq-universal-sybr-green-one-step-kit-5ml-172-5151-view.jpg ,VN Molly Gregg iTaq Universal SYBR Green One-Step Kit 5 ml (5 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR Green reaction mix (Taq polymerase, buffers, ROX normalization dyes), iScript reverse transcriptase, and nuclease-free water, for 500 x 20 µl reactions 1725151, itaq, universal, sybr, green, one-step, one, step, rt-pcr, reverse, transcription, real-time, real, time, pcr, qpcr, detection, amplification, gene, expression 06/20/13 12:21 PM 06/20/23 12:30 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Our Universal Real-Time PCR Reagents Web App brings you in-depth product information, individual and competitive performance data, interactive tutorials, 3-D animated videos, technical manuals, and more.</p> <p>Use our combined iScript&trade; RNase H+reverse transcriptase and antibody hot-start iTaq DNA polymerase kit formulation to complete your SYBR<sup>&reg;</sup> Green real-time reactions in one step &mdash; PCR efficiency is improved over a wide dynamic range and under various conditions.</p> <ul> <li>Advanced formulation for amplifying difficult targets and challenging samples</li> <li>Can be combined with our <a href="/evportal/destination/commerce/product_detail?catID=M0HROA15">PrimePCR&trade; assays</a> for a simplified workflow</li> <li>Universal &mdash; works on any real-time PCR system</li> </ul> <h2 class="descHeader">Kit Components</h2> <ul> <li>Reverse transcriptase &ndash; 125 &micro;l</li> <li>Reaction mix &ndash; 5 ml (5 x 1 ml)</li> </ul> <h2 class="descHeader">Instrument Compatibility</h2> <p>iTaq Universal SYBR Green One-Step RT-qPCR Kit&nbsp;is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0*</li> </ul> </td> </tr> <tr> <td valign="top">BioFire</td> <td valign="top"> <ul> <li>LightScanner HR-1</li> <li>LightScanner 32*</li> </ul> </td> </tr> </tbody> </table> <p class="caption caption-margin">* BSA must be added according to instrument specifications</p> <h2 class="descHeader">Applications and Uses of the iTaq<sup>&reg;</sup> Universal SYBR<sup>&reg;</sup> Green One-Step Kit</h2> <ul> <li>qPCR/real-time PCR</li> <li>Gene expression analysis</li> <li>Absolute quantification</li> <li>Mutation detection</li> <li>Pathogen detection</li> <li>Viral detection (load)</li> <li>Genetically modified organism (GMO) characterization</li> <li>Genetic profiling</li> </ul> <h2 class="descHeader">Packaging Options</h2> <p>The <strong>iTaq&trade; universal SYBR<sup>&reg;</sup> Green one-step kit</strong> is also available in a format of <a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5150">100 reactions (171-5150)</a>.</p> <h2 class="descHeader">Related Products</h2> <p>Bio-Rad has also developed one-step kits for <a href="/en-us/product/itaq-universal-probes-one-step-kit">probe-specific applications</a>.</p> /webroot/web/html/lsr/products/SKUs 172-5151.html /webroot/web/html/lsr/products/SKUs/172-5151.html Product expiration (after shipping date) 2026 12 months 1 0 RT storage at –20°C (after shipping date) 2898 12 months 1 0 Reaction mix storage at 4°C (after thawing) 2896 Up to 3 months 1 0 Customer guarantee (minimum usage time) at –20°C 2530 12 months 1 0 false $836.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725151&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725151&CartQuote=QT&quantity= 1468 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725151&AddToDefaultWishList=true&quantity= 41106300 836.00 Product detail page 1725151
List Price: 
 
$836.00 
LoadingLoading...
EDU Price:
  What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
 
iTaq Universal Probes One-Step Kit, 100 x 20 µl rxns, 1 ml

1725140
100 x 20 µl reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water

1725140 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5140_1373330820.xml 1725140 07/08/13 05:44 PM en_US true internet/product_SKU 2020-05-27 00:10:40 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5140_1373330820.xml /default/main/bio-rad/WORKAREA/default jcrucyta 2017-11-14 07:07:36 true jcrucyta templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5140_1373330820.xml LSR N /default/main/bio-rad en 07/10/23 05:46 PM 1590367401544 2020-05-27 00:10:40 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5140_1373330820.xml 1725140-itaq-universal-probes-one-step-kit-100-x-20-ul-rxns-1-ml 1 1725140 ITAQ UNIV PROBE 1 STEP 100RX iTaq Universal Probes One-Step Kit, 100 x 20 µl rxns, 1 ml 100 x 20 &micro;l reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water 100 x 20 &micro;l reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/itaq-universal-probes-one-step-kit-172-5140-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/itaq-universal-probes-one-step-kit-172-5140.jpg Karen Moss iTaq Universal Probes One-Step Kit 100 x 20 &micro;l reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water 1725140, itaq, universal, probes, one-step 100x20, reactions, one, step, rt-pcr, reverse, transcription, real-time, real, time, pcr, qpcr, detection, amplification, gene, expression 07/08/13 05:44 PM 07/10/23 05:46 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use our combined iScript RNase H<sup>+</sup> reverse transcriptase and antibody hot-start iTaq DNA polymerase probe kit formulation to complete your real-time reactions in one step &mdash; PCR efficiency is improved over a wide dynamic range and under various conditions.</p> <!--ul> <li>Advanced formulation for amplifying difficult target sequences and challenging samples</li> <li>Perform singleplex or multiplex reactions simultaneously</li> <li>Normalize qPCR data with ROX normalization dyes</li> </ul--> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Sensitive and reproducible <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/greater_sensitivity_ov.html')">singleplex or multiplex</a> reactions directly from RNA</li> <li>Advanced formulation for amplifying <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/high_linearity_ov.html')">difficult target</a> sequences and challenging samples</li> <li>Tested with ethanol for <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/better_inhibitor_ov.html')">inhibitor tolerance</a> and compatible with up to 4 &micro;l crude lysate</li> <li>Works on any real-time PCR system</li> </ul> <h2 class="descHeader">Kit Components</h2> <ul> <li>Reverse transcriptase &mdash; 50 &micro;l</li> <li>Reaction mix &mdash; 1 ml</li> </ul> <h2 class="descHeader">Instrument Compatibility</h2> <p>iTaq Universal Probes One-Step RT-qPCR Kit is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0*</li> </ul> </td> </tr> <tr> <td valign="top">BioFire</td> <td valign="top"> <ul> <li>LightScanner HR-1</li> <li>LightScanner 32*</li> </ul> </td> </tr> </tbody> </table> <p class="caption caption-margin">* BSA must be added according to instrument specifications</p> <h2 class="descHeader">Applications and Uses of the iTaq Universal Probes One-Step Kit</h2> <ul> <li>qPCR/real-time PCR</li> <li>Gene expression analysis</li> <li>Multiplexing</li> <li>Absolute quantification</li> <li>Mutation detection</li> <li>Pathogen detection</li> <li>Viral detection (load)</li> <li>Genetically modified organisms (GMO) characterization</li> <li>Genetic profiling</li> </ul> <h2 class="descHeader">Packaging Options</h2> <p>The <strong>iTaq Universal Probes One-Step Kit</strong> is also available in a format of 500 reactions (<a href="/en-us/sku/172-5141-itaq-universal-probes-one-step-kit-500-x-20-ul-reactions">1725141</a>) and 5,000 reactions (<a href="/en-us/sku/12013250-itaq-universal-probes-one-step-kit-5000-x-20-ul-rxns?ID=12013250 ">12013250</a>).</p> <h2 class="descHeader">Related Products</h2> <p>Bio-Rad has also developed one-step kits for <a href="/en-us/product/itaq-universal-sybr-green-one-step-kits">SYBR<sup>&reg;</sup> Green specific applications</a>.</p> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global itaq-universal-probes-one-step-kit-172-5140-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/itaq-universal-probes-one-step-kit-172-5140-desc.html Product format 3184 Two-tube system 1 0 Kit content 3187 iScript reverse transcriptase + 2x iTaq PCR reaction mix 1 0 Detection chemistry 3185 SYBR Green or probes 1 0 Multiplex capability (for probes only) 3188 Duplex (triplex possible depending on the abundance of the targets) 1 0 Inhibitor tolerance 3180 Tested with ethanol. Also compatible with up to 4 &micro;l of crude lysates 1 0 5’ to 3’ exonuclease activity of the enzyme(s) 3182 Yes 1 0 Hot-start, activation time 3189 Yes, 1&ndash;3 min 1 0 Sample input 3179 100 fg to 100 ng 1 0 Sensitivity 2137 10 copies in a duplex reaction 1 0 Reference dye 3186 Patented universal dye preblended in the iTaq reaction mix 1 0 Instrument compatibility 2752 Both ROX-dependent (high- and low-) and -independent instruments 1 0 Product expiration (after shipping date) 2026 12 months 1 0 Reaction mix storage at 4°C (after thawing) 2896 Up to 3 months 1 0 RT storage at –20°C (after shipping date) 2898 12 months 1 0 Customer guarantee (minimum usage time) at –20°C 2530 12 months 1 0 false $229.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725140&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725140&CartQuote=QT&quantity= 1468 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725140&AddToDefaultWishList=true&quantity= 41106300 229.00 Product detail page 1725140
List Price: 
 
$229.00 
LoadingLoading...
EDU Price:
  What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
 
iTaq Universal Probes One-Step Kit, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725141
500 x 20 µl reactions, 5 ml (5 x 1 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water

1725141 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5141_1373330944.xml 1725141 07/08/13 05:47 PM en_US true internet/product_SKU 2020-05-27 00:10:40 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5141_1373330944.xml /default/main/bio-rad/WORKAREA/default jcrucyq4 2017-11-14 07:07:36 true jcrucyq4 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5141_1373330944.xml LSR N /default/main/bio-rad en 07/10/23 05:48 PM 1590367372289 2020-05-27 00:10:40 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5141_1373330944.xml 1725141-itaq-universal-probes-one-step-kit-500-x-20-ul-rxns-5-ml-5-x-1-ml 1 1725141 iTaq Universal Probes One-Step Kit, 500 rxn iTaq Universal Probes One-Step Kit, 500 x 20 µl rxns, 5 ml (5 x 1 ml) 500 x 20 &micro;l reactions, 5 ml (5 x 1 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water 500 x 20 &micro;l reactions, 5 ml (5 x 1 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/itaq-universal-probes-one-step-kit-172-5141-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/itaq-universal-probes-one-step-kit-172-5141.jpg Karen Moss iTaq Universal Probes One-Step Kit 500 x 20 &micro;l reactions, 5 ml (5 x 1 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water 1725141, itaq, universal, probes, one-step, 500x20, reactions, one, step, rt-pcr, reverse, transcription, real-time, real, time, pcr, qpcr, detection, amplification, gene, expression 07/08/13 05:47 PM 07/10/23 05:48 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use our combined iScript RNase H<sup>+</sup> reverse transcriptase and antibody hot-start iTaq DNA polymerase probe kit formulation to complete your real-time reactions in one step &mdash; PCR efficiency is improved over a wide dynamic range and under various conditions.</p> <!--ul> <li>Advanced formulation for amplifying difficult target sequences and challenging samples</li> <li>Perform singleplex or multiplex reactions simultaneously</li> <li>Normalize qPCR data with ROX normalization dyes</li> </ul--> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Sensitive and reproducible <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/greater_sensitivity_ov.html')">singleplex or multiplex</a> reactions directly from RNA</li> <li>Advanced formulation for amplifying <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/high_linearity_ov.html')">difficult target</a> sequences and challenging samples</li> <li>Tested with ethanol for <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/better_inhibitor_ov.html')">inhibitor tolerance</a> and compatible with up to 4 &micro;l crude lysate</li> <li>Works on any real-time PCR system</li> </ul> <h2 class="descHeader">Kit Components</h2> <ul> <li>Reverse transcriptase &mdash; 250 &micro;l</li> <li>Reaction mix &mdash; 5 ml (5 x 1 ml)</li> </ul> <h2 class="descHeader">Instrument Compatibility</h2> <p>iTaq Universal Probes One-Step RT-qPCR Kit is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0*</li> </ul> </td> </tr> <tr> <td valign="top">BioFire</td> <td valign="top"> <ul> <li>LightScanner HR-1</li> <li>LightScanner 32*</li> </ul> </td> </tr> </tbody> </table> <p class="caption caption-margin">* BSA must be added according to instrument specifications</p> <h2 class="descHeader">Applications and Uses of the iTaq Universal Probes One-Step Kit</h2> <ul> <li>qPCR/real-time PCR</li> <li>Gene expression analysis</li> <li>Multiplexing</li> <li>Absolute quantification</li> <li>Mutation detection</li> <li>Pathogen detection</li> <li>Viral detection (load)</li> <li>Genetically modified organisms (GMO) characterization</li> <li>Genetic profiling</li> </ul> <h2 class="descHeader">Packaging Options</h2> <p>The <strong>iTaq universal probes one-step kit</strong> is also available in a format of 100 reactions (<a href="/en-us/sku/172-5140-itaq-universal-probes-one-step-kit-100-x-20-ul-reactions">1725140</a>) and 5,000 reactions (<a href="/en-us/sku/12013250-itaq-universal-probes-one-step-kit-5000-x-20-ul-rxns?ID=12013250 ">12013250</a>).</p> <h2 class="descHeader">Related Products</h2> <p>Bio-Rad has also developed one-step kits for <a href="/en-us/product/itaq-universal-sybr-green-one-step-kits">SYBR<sup>&reg;</sup>Green specific applications</a>.</p> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global itaq-universal-probes-one-step-kit-172-5141-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/itaq-universal-probes-one-step-kit-172-5141-desc.html Product format 3184 Two-tube system 1 0 Kit content 3187 iScript reverse transcriptase + 2x iTaq PCR reaction mix 1 0 Detection chemistry 3185 SYBR Green or probes 1 0 Multiplex capability (for probes only) 3188 Duplex (triplex possible depending on the abundance of the targets) 1 0 Inhibitor tolerance 3180 Tested with ethanol. Also compatible with up to 4 &micro;l of crude lysates 1 0 5’ to 3’ exonuclease activity of the enzyme(s) 3182 Yes 1 0 Hot-start, activation time 3189 Yes, 1&ndash;3 min 1 0 Sample input 3179 100 fg to 100 ng 1 0 Sensitivity 2137 10 copies in a duplex reaction 1 0 Reference dye 3186 Patented universal dye preblended in the iTaq reaction mix 1 0 Instrument compatibility 2752 Both ROX-dependent (high- and low-) and -independent instruments 1 0 Product expiration (after shipping date) 2026 12 months 1 0 Reaction mix storage at 4°C (after thawing) 2896 Up to 3 months 1 0 RT storage at –20°C (after shipping date) 2898 12 months 1 0 Customer guarantee (minimum usage time) at –20°C 2530 12 months 1 0 false $836.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725141&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725141&CartQuote=QT&quantity= 1468 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725141&AddToDefaultWishList=true&quantity= 24121500 836.00 Product detail page 1725141
List Price: 
 
$836.00 
LoadingLoading...
EDU Price:
  What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
 
iTaq Universal Probes One-Step Kit, 5,000 x 20 µl rxns

12013250
5,000 x 20 µl reactions, 50 ml (1 x 50 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; 2.5 ml (1 x 2.5 ml) iScript reverse transcriptase; and 45 ml (1 x 45 ml) nuclease-free water

12013250 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12013250_1590189257.xml 12013250 05/22/20 11:07 PM en_US true internet/product_SKU 2020-05-27 00:10:40 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12013250_1590189257.xml /default/main/bio-rad/WORKAREA/default k9m07yrq 2020-05-22 23:14:18 true k9m07yrq templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12013250_1590189257.xml LSR N /default/main/bio-rad en 05/22/37 01:13 PM 1590367350156 2020-05-27 00:10:40 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12013250_1590189257.xml 12013250-itaq-universal-probes-one-step-kit-5000-x-20-ul-rxns 1 12013250 iTaq Universal Probes 1-Step Kt, 5000 Rx iTaq Universal Probes One-Step Kit, 5,000 x 20 µl rxns 5,000 x 20 µl reactions, 50 ml (1 x 50 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; 2.5 ml (1 x 2.5 ml) iScript reverse transcriptase; and 45 ml (1 x 45 ml) nuclease-free water 5,000 x 20 µl reactions, 50 ml (1 x 50 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; 2.5 ml (1 x 2.5 ml) iScript reverse transcriptase; and 45 ml (1 x 45 ml) nuclease-free water preview TW 12013250 5,000 x 20 µl reactions, 50 ml (1 x 50 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; 2.5 ml (1 x 2.5 ml) iScript reverse transcriptase; and 45 ml (1 x 45 ml) nuclease-free water 1725141, itaq, universal, probes, one-step, 500x20, reactions, one, step, rt-pcr, reverse, transcription, real-time, real, time, pcr, qpcr, detection, amplification, gene, expression 05/22/20 11:07 PM 05/22/37 01:13 PM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use our combined iScript RNase H<sup>+</sup> reverse transcriptase and antibody hot-start iTaq DNA polymerase probe kit formulation to complete your real-time reactions in one step &mdash; PCR efficiency is improved over a wide dynamic range and under various conditions.</p> <!--ul> <li>Advanced formulation for amplifying difficult target sequences and challenging samples</li> <li>Perform singleplex or multiplex reactions simultaneously</li> <li>Normalize qPCR data with ROX normalization dyes</li> </ul--> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Sensitive and reproducible <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/greater_sensitivity_ov.html')">singleplex or multiplex</a> reactions directly from RNA</li> <li>Advanced formulation for amplifying <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/high_linearity_ov.html')">difficult target</a> sequences and challenging samples</li> <li>Tested with ethanol for <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/better_inhibitor_ov.html')">inhibitor tolerance</a> and compatible with up to 4 &micro;l crude lysate</li> <li>Works on any real-time PCR system</li> </ul> <h2 class="descHeader">Kit Components</h2> <ul> <li>Reverse transcriptase &mdash; 2.5 ml (1 x 2.5 ml)</li> <li>Reaction mix &mdash; 50 ml (1 x 50 ml)</li> <li>Nuclease-free water &mdash; 45 ml (1 x 45 ml)</li> </ul> <h2 class="descHeader">Instrument Compatibility</h2> <p>iTaq Universal Probes One-Step RT-qPCR Kit is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0*</li> </ul> </td> </tr> <tr> <td valign="top">BioFire</td> <td valign="top"> <ul> <li>LightScanner HR-1</li> <li>LightScanner 32*</li> </ul> </td> </tr> </tbody> </table> <p class="caption caption-margin">* BSA must be added according to instrument specifications</p> <h2 class="descHeader">Applications and Uses of the iTaq Universal Probes One-Step Kit</h2> <ul> <li>qPCR/real-time PCR</li> <li>Gene expression analysis</li> <li>Multiplexing</li> <li>Absolute quantification</li> <li>Mutation detection</li> <li>Pathogen detection</li> <li>Viral detection (load)</li> <li>Genetically modified organisms (GMO) characterization</li> <li>Genetic profiling</li> </ul> <h2 class="descHeader">Packaging Options</h2> <p>The <strong>iTaq universal probes one-step kit</strong> is also available in a format of 100 reactions (<a href="/en-us/sku/172-5140-itaq-universal-probes-one-step-kit-100-x-20-ul-reactions">1725140</a>) and 500 reactions (<a href="/en-us/sku/1725141-itaq-universal-probes-one-step-kit-500-x-20-ul-rxns-5-ml-5-x-1-ml?ID=1725141">1725141</a>).</p> <h2 class="descHeader">Related Products</h2> <p>Bio-Rad has also developed one-step kits for <a href="/en-us/product/itaq-universal-sybr-green-one-step-kits">SYBR<sup>&reg;</sup>Green specific applications</a>.</p> Product format 3184 Two-tube system 1 0 Kit content 3187 iScript reverse transcriptase + 2x iTaq PCR reaction mix 1 0 Detection chemistry 3185 SYBR Green or probes 1 0 Multiplex capability (for probes only) 3188 Duplex (triplex possible depending on the abundance of the targets) 1 0 Inhibitor tolerance 3180 Tested with ethanol. Also compatible with up to 4 &micro;l of crude lysates 1 0 5’ to 3’ exonuclease activity of the enzyme(s) 3182 Yes 1 0 Hot-start, activation time 3189 Yes, 1&ndash;3 min 1 0 Sample input 3179 100 fg to 100 ng 1 0 Sensitivity 2137 10 copies in a duplex reaction 1 0 Reference dye 3186 Patented universal dye preblended in the iTaq reaction mix 1 0 Instrument compatibility 2752 Both ROX-dependent (high- and low-) and -independent instruments 1 0 Product expiration (after shipping date) 2026 12 months 1 0 RT storage at –20°C (after shipping date) 2898 12 months 1 0 Reaction mix storage at 4°C (after thawing) 2896 Up to 3 months 1 0 Customer guarantee (minimum usage time) at –20°C 2530 12 months 1 0 false $7,524.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12013250&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12013250&CartQuote=QT&quantity= 1468 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12013250&AddToDefaultWishList=true&quantity= 24121500 7524.00 Product detail page 12013250
List Price: 
 
$7,524.00 
LoadingLoading...
EDU Price:
  What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
 
Number Description Options
10032048 iTaq™ Universal SYBR® Green One-Step Kit Product Insert, Rev B Click to download
10032046 iTaq Universal Probes One-Step Kit Product Insert, Rev B Click to download
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
7178 Amplification: Real-Time PCR Reagents and Kits Brochure Click to download
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
6516 Better, Faster, and Less Expensive Gene Expression Analysis Using Multiplex One-Step RT-qPCR: A Case Study of Colorectal Cancer, Rev A Click to download

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Get Help
Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.
NVENWV15 [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [54.160.19.155, 10.232.19.102] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [if-modified-since] = [Mon, 26 Aug 2019 09:47:53 UTC] [seourl] = [/en-us/product/1-step-quantitative-reverse-transcription-pcr-rt-qpcr-rna] [x-amzn-trace-id] = [Root=1-5f2cae51-03a0a7e82953ed741941cd4c] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=NVENWV15&WT.mc_id=180911025112] [host] = [10.232.1.21:1776] [x-request-uri] = [/en-us/product/1-step-quantitative-reverse-transcription-pcr-rt-qpcr-rna] [connection] = [Keep-Alive] [accept-encoding] = [br,gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] 180911025112 Catalog/ProductDetail pageStyleKey 1-step-quantitative-reverse-transcription-pcr-rt-qpcr-rna LSR https://www.bio-rad-antibodies.com https://www.bio-rad.com 03/26/14 02:17 PM en_US true internet/generic 2020-08-05 00:02:29 /default/main/bio-rad/STAGING 1385504243813 US templatedata/internet/generic/data/en_US/GNL/page_generic_footer_b2c_1385505294_US.xml page_generic_footer_b2c /default/main/bio-rad/WORKAREA/default jc7v8okm 2017-10-27 13:13:40 true Done jc7v8okm templatedata/internet/generic/data/en_US/GNL/page_generic_footer_b2c_1385505294_US.xml GNL N /default/main/bio-rad en_US 11/27/23 02:18 PM 1594193511948 2020-08-05 00:02:29 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/en_US/GNL/page_generic_footer_b2c_1385505294_US.xml footer 1 1385504243813 page_generic_footer_b2c <div class="col"> <h4>Support</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research/support?ID=1140">Life Science Support</a></li> <li><a class="wtclass linkgeneration" href="_acdDomain/technical-support.html">Antibodies Support</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research/purchase-service-programs/expert-care-service?ID=1383780912356">Expert Care Service</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/contact">Contact Us</a></li> </ul> </div> <div class="col"> <h4>Documents</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/literature-library">Literature Library</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research/support/certificate-of-analysis">Certificate of Analysis</a></li> <li><a class="wtclass" href="https://myeinserts.qcnet.com/" target="_blank" rel="noopener noreferrer">QC Inserts</a></li> <li><a class="wtclass linkgeneration" href="/featured/en/quality-management-systems-certificates.html">Quality Certification</a></li> </ul> </div> <div class="col"> <h4>Ordering</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/my-bio-rad">Quick Order</a></li> <li><a id="footer_orderLink" class="orderhistoryLink wtclass" href="javascript:void(0);">Order History</a></li> <li><a class="wtclass" id="footer_quoteLink" href="javascript:void(0);">Quote History</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/my-bio-rad">My Bio-Rad</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research/purchase-service-programs/supply-center-program?ID=1383773882551">Supply Centers</a></li> </ul> </div> <div class="col"> <h4>Our Products</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research?ID=1100">Life Science Research</a></li> <li><a class="wtclass linkgeneration" href="_acdDomain/">Antibodies</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/clinical-diagnostics?ID=1200">Clinical Diagnostics</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/process-separations?ID=1400">Process Separations</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/food-science?ID=1475">Food Science</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/education?ID=1450">Life Science Education</a></li> </ul> </div> <div class="col" style="padding-right: 0;"> <h4>Company</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/corporate/about-bio-rad?ID=1003">About Bio-Rad</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/corporate/careers?ID=1004">Careers</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/corporate/investor-relations?ID=1007">Investor Relations</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/corporate/newsroom?ID=1006">Newsroom</a></li> <li><a class="wtclass linkgeneration" href="_acdDomain/blog/">Blog</a></li> <li><a class="wtclass" target="_blank" href="https://www.bioradiations.com" rel="noopener noreferrer">Bioradiations</a></li> <li> <div class="social"> <ul> <li><a class="no-target-style brconly wtclass" href="https://www.linkedin.com/company/bio-rad?trk=top_nav_home" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/linkedin_round_40x40.png" alt="Bio-Rad LinkedIn" width="20" height="20" /></a> <a class="no-target-style bracd wtclass" style="display: none;" href="https://www.linkedin.com/company/bio-rad-antibodies" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/linkedin_round_40x40.png" alt="Bio-Rad Antibodies LinkedIn" width="20" height="20" /></a></li> <li><a class="no-target-style brconly wtclass" href="https://www.youtube.com/user/BioRadLifeScience" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/youtube-icon-rnd-40x40.png" alt="Bio-Rad YouTube" width="20" height="20" /></a> <a class="no-target-style bracd wtclass" style="display: none;" href="https://www.youtube.com/playlist?list=PLrAEgIY86I6w1jajKp3O9RQJEvJZ2ux0I" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/youtube-icon-rnd-40x40.png" alt="Bio-Rad Antibodies YouTube" width="20" height="20" /></a></li> <li><a class="no-target-style brconly wtclass" href="https://twitter.com/BioRadLifeSci" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/twitter_round_40x40.png" alt="Bio-Rad Twitter" width="20" height="20" /></a> <a class="no-target-style bracd wtclass" style="display: none;" href="https://twitter.com/BioRadAbs" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/twitter_round_40x40.png" alt="Bio-Rad Antibodies Twitter" width="20" height="20" /></a></li> <li><a class="no-target-style brconly wtclass" href="https://www.facebook.com/biorad/" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/facebook-icon-rnd-40x40.png" alt="Bio-Rad Facebook" width="20" height="20" /></a> <a class="no-target-style bracd wtclass" style="display: none;" href="https://www.facebook.com/BioRadAntibodies" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/facebook-icon-rnd-40x40.png" alt="Bio-Rad Antibodies Facebook" width="20" height="20" /></a></li> <li><a class="no-target-style wtclass" href="https://www.instagram.com/bioradlabs/" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/instagram-icon-rnd-40x40.png" alt="Bio-Rad Instagram" width="20" height="20" /></a></li> <li><a class="no-target-style wtclass" href="https://uk.pinterest.com/BioRadAbs/" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/pinterest40x40.png" alt="Bio-Rad Pinterest" width="20" height="20" /></a></li> <li><a class="no-target-style wtclass" href="https://info.bio-rad.com/WeChat.html" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/wechat_round_40x40.png" alt="Bio-Rad WeChat" width="20" height="20" /></a></li> </ul> </div> </li> </ul> </div> 0 /webroot/web/html footer_page_b2c_en_US.html /webroot/web/html/footer_page_b2c_en_US.html Karen Moss Footer page Footer 03/26/14 02:17 PM 11/27/23 02:18 PM US en GNL /GNL N 0 en-us Home <script type="text/javascript">$(document).ready(function() { var region = $("#headlangID .lang strong").html(); $(".footer-nav > a:nth-child(3)").after(" | <a href='https://www.bio-rad.com/webroot/web/html/featured/en/web-accessibility.html' id='regionlink'>Web Accessibility</a>");});</script>Trademarks Site Terms Terms & Conditions Imprint EU Recycle Program Transparency Information Privacy Change Cookie Setting <script type="text/javascript">$(document).ready(function () {$(".links2").append('<style>.social-links-br {float: left;margin-right: 10px;}.social-links-br img {width: 20px;height: 20px;margin-right: 5px}</style><div class="social-links-br"><a class="no-target-style" href="https://www.linkedin.com/company/bio-rad?trk=top_nav_home" target="_blank"><img src="/webroot/web/images/general/icons/linkedin_round_40x40.png" alt="Bio-Rad LinkedIn"></a><a class="no-target-style" href="https://www.youtube.com/user/BioRadLifeScience" target="_blank"><img src="/webroot/web/images/general/icons/youtube-icon-rnd-40x40.png" alt="Bio-Rad YouTube"></a><a class="no-target-style" href="https://twitter.com/BioRadLifeSci" target="_blank"><img src="/webroot/web/images/general/icons/twitter_round_40x40.png" alt="Bio-Rad Twitter"></a><a class="no-target-style" href="https://www.facebook.com/biorad/" target="_blank"><img src="/webroot/web/images/general/icons/facebook-icon-rnd-40x40.png" alt="Bio-Rad Facebook"></a><a class="no-target-style" href="https://www.instagram.com/bioradlabs/" target="_blank"><img src="/webroot/web/images/general/icons/instagram-icon-rnd-40x40.png" alt="Bio-Rad Instagram"></a></div>');});</script>&copy; {0} Bio-Rad Laboratories, Inc. This page was last edited on {0} true True GTM-MJ2DN2S us US en LSR life-science-research life-science-research /prd/en/US/direct/biorad?ts=1&cmd=OrdersWorkspaceDisplay /prd/en/US/direct/biorad?ts=1&cmd=QuotesWorkspaceDisplay /prd/en/US/direct/biorad?ts=1&cmd=InvoiceWorkspaceDisplay&_Tab=Invoice false false true true false 1040988879 LHsYCOu_wQMQz_Ww8AM window.google_tag_params true 272-THL-329 EU_COOKIE_PREFS CartID_ WT_FPC <script type="text/javascript">$.ajax({url: document.location.protocol + '//munchkin.marketo.net/munchkin.js',dataType: 'script',cache: true,success: function() {Munchkin.init('272-THL-329');}});</script> <script type="text/javascript" src="https://www.bio-rad.com/evportal/framework/skins/evolution/js/proxyMunchkin.js"></script> 2020 {"country":"us","b2b_punch_out_mode":"","pageType":"Product Detail Page","user_id":"","visitorLoginState":false,"visitorType":"","sessionType":"B2C","language":"en","vertical":"LSR","b2b_org_id":""} 0 0 0