Ask the Expert Webcast

Speaker:
Heidi Jones, MBA
Market Development Manager,
Chromatography, Bio-Rad Laboratories

Attend Our Upcoming Ask The Expert Webcast

Traditional Protein A affinity chromatography is the gold standard for Fc-fusion protein purification, but it can result in protein denaturation and degradation due to low-pH elution. In this Ask the Expert webcast, Heidi Jones will discuss a two-step purification workflow using new, weak anion exchange-hydrophobic resin, Nuvia™ wPrime 2A, followed by a strong anion exchange resin, Nuvia HP-Q, for the purification of EGFR:Fc fusion proteins. Together, this two-step process offers a non-affinity-based alternative that achieves high purity and yield of EGFR:Fc fusion proteins while preserving protein integrity.

Key Takeaways:

• Mixed-mode and anion exchange chromatography achieve purity and yield comparable to Protein A methods, offering more flexibility
• Neutral pH purification reduces the risk of Fc-fusion protein denaturation and degradation
• Fc-fusion proteins enable alternative purification strategies when traditional affinity chromatography is unsuitable

Register Now for Gentler Workflow: Fc-Fusion Protein Purification with Mixed-Mode and Anion Exchange Chromatography, Text