Gene:  PRAME, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues except testis. This expression pattern is similar to that of other CT antigens such as MAGE BAGE and GAGE. However unlike these other CT antigens this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: PRAME, Human
preferentially expressed antigen in melanoma

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0021741
Info:   Same primer pair as used in probe assay qHsaCIP0033165
List Price:    $138.00
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PrimePCR™ Probe Assay: PRAME, Human
preferentially expressed antigen in melanoma

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0033165
List Price:    $202.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PRAME, Human
preferentially expressed antigen in melanoma

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5024142
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0021741; Intron-spanning
List Price:    $138.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PRAME, Human
preferentially expressed antigen in melanoma

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5057658
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0033165; Intron-spanning
 
Fluorophore:   FAM
List Price:    $207.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PRAME, Human
preferentially expressed antigen in melanoma

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5057659
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0033165; Intron-spanning
 
Fluorophore:   HEX
List Price:    $207.00
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PrimePCR™ ddPCR™ Mutation Assay:PRAME p.W7R, Human
preferentially expressed antigen in melanoma

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504088
Info:   65nt amplicon; crosses exon-intron junction; in silico
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PrimePCR™ ddPCR™ Mutation Assay:PRAME WT for p.W7R, Human
preferentially expressed antigen in melanoma

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504089
Info:   65nt amplicon; crosses exon-intron junction; in silico
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