Gene:  MTHFS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 510-methenyltetrahydrofolate a precursor of reduced folates involved in 1-carbon metabolism. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Jun 2011]

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PrimePCR™ SYBR® Green Assay: MTHFS, Human
5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0002426
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PrimePCR™ SYBR® Green Assay: MTHFS, Human
5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044691
Info:   Same primer pair as used in probe assay qHsaCEP0051953
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Add to Custom Plate
PrimePCR™ Probe Assay: MTHFS, Human
5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051953
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MTHFS, Human
5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5001139
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0002426; Exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MTHFS, Human
5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5009316
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0044691; exonic
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PrimePCR™ ddPCR™ Expression Probe Assay: MTHFS, Human
5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036944
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0051953; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: MTHFS, Human
5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036945
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0051953; exonic
 
Fluorophore:   HEX
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    Related pathways not available
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