Gene:  MED12, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene mediator complex subunit 12 (MED12) along with MED13 CDK8 kinase and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome also known as FG syndrome and Lujan-Fryns syndrome. [provided by RefSeq Aug 2009]

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PrimePCR™ SYBR® Green Assay: MED12, Human
mediator complex subunit 12

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0001926
Info:   Same primer pair as used in probe assay qHsaCEP0024217
List Price:    $138.00
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PrimePCR™ Probe Assay: MED12, Human
mediator complex subunit 12

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0024217
List Price:    $202.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MED12, Human
mediator complex subunit 12

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5000745
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0001926; Exonic
List Price:    $138.00
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PrimePCR™ ddPCR™ Mutation Assay: MED12 p.G44D, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506806
Info:   65nt amplicon; exonic
List Price:    $207.00
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PrimePCR™ ddPCR™ Mutation Assay: MED12 WT for p.G44D, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506807
Info:   65nt amplicon; exonic
List Price:    $207.00
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PrimePCR™ ddPCR™ Mutation Assay: MED12 p.G44V, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506886
Info:   65nt amplicon; exonic
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PrimePCR™ ddPCR™ Mutation Assay: MED12 WT for p.G44V, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506887
Info:   65nt amplicon; exonic
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PrimePCR™ ddPCR™ Expression Probe Assay: MED12, Human
mediator complex subunit 12

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5025802
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0024217; Exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: MED12, Human
mediator complex subunit 12

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5025803
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0024217; Exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:MED12 p.G44V, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502008
Info:   65nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:MED12 WT for p.G44V, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502009
Info:   65nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:MED12 p.L1224F, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502838
Info:   61nt amplicon; crosses exon-intron junction; in silico
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PrimePCR™ ddPCR™ Mutation Assay:MED12 WT for p.L1224F, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502839
Info:   61nt amplicon; crosses exon-intron junction; in silico
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PrimePCR™ ddPCR™ Mutation Assay:MED12 p.G44D, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503752
Info:   65nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:MED12 WT for p.G44D, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503753
Info:   65nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:MED12 p.G44A, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504106
Info:   65nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:MED12 WT for p.G44A, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504107
Info:   65nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:MED12 p.L36R, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505014
Info:   70nt amplicon; crosses exon-intron junction; in silico
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PrimePCR™ ddPCR™ Mutation Assay:MED12 WT for p.L36R, Human
mediator complex subunit 12

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505015
Info:   70nt amplicon; crosses exon-intron junction; in silico
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