Gene:  LIMK2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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There are approximately 40 known eukaryotic LIM proteins so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK a downstream effector of Rho and the encoded protein in turn phosphorylates cofilin inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: LIMK2, Human
LIM domain kinase 2

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0022037
Info:   Same primer pair as used in probe assay qHsaCIP0033265
List Price:    $138.00
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PrimePCR™ Probe Assay: LIMK2, Human
LIM domain kinase 2

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0033265
List Price:    $202.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: LIMK2, Human
LIM domain kinase 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5024259
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0022037; Intron-spanning
List Price:    $138.00
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PrimePCR™ ddPCR™ Expression Probe Assay: LIMK2, Human
LIM domain kinase 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5057772
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0033265; Intron-spanning
 
Fluorophore:   FAM
List Price:    $207.00
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PrimePCR™ ddPCR™ Expression Probe Assay: LIMK2, Human
LIM domain kinase 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5057773
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0033265; Intron-spanning
 
Fluorophore:   HEX
List Price:    $207.00
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PrimePCR™ ddPCR™ Mutation Assay:LIMK2 p.R364*, Human
LIM domain kinase 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503710
Info:   78nt amplicon; crosses exon-intron junction; in silico
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PrimePCR™ ddPCR™ Mutation Assay:LIMK2 WT for p.R364*, Human
LIM domain kinase 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503711
Info:   78nt amplicon; crosses exon-intron junction; in silico
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