Gene:  IDH1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases which localize to the mitochondrial matrix and two NADP(+)-dependent isocitrate dehydrogenases one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions such as the conversion of 2 4-dienoyl-CoAs to 3-enoyl-CoAs as well as in peroxisomal reactions that consume 2-oxoglutarate namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: IDH1, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044840
Info:   Same primer pair as used in probe assay qHsaCEP0052102
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PrimePCR™ SYBR® Green Assay: IDH1, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012601
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PrimePCR™ Probe Assay: IDH1, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052102
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