Gene: Gnas, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

Gene Symbol: Gnas
Aliases: 5530400H20Rik, A930027G11Rik, C130027O20Rik, GPSA, GSP, Galphas, Gnas1, Gnasxl, Gsa, Nesp, Nespl, Oed-Sml, Oedsml, P1, P2, P3, PHP1A, PHP1B, POH
RefSeq: NC_000068.7 NT_039207.8
Ensembl: ENSMUSG00000027523
Entrez: 14683
UniGene: Mm.125770

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Design and Validation of Real-Time PCR Primers-test

Pathway Curation
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Control and Reference Assays for Real-Time PCR

Control assays and synthetic DNA templates were designed to facilitate the assessment of the key experimental factors impacting your real-time PCR results.

DNA Contamination Control Assay
Use the PrimePCR DNA contamination control assay to determine if genomic DNA (gDNA) is present in a sample at a level that may affect PCR results. This assay may also be used to compare relative levels of gDNA contamination present in different samples to determine if PCR results may be affected.

Positive PCR Control Assay
Use the PrimePCR positive control assay to qualitatively assess the performance of a PCR reaction associated with a single sample. This assay may also be used to compare the relative performance of PCR reactions associated with different samples.

RNA Quality Assay
Use the PrimePCR RNA quality assay to determine if RNA integrity may adversely affect PCR results for a single sample. This assay may also be used to compare relative RNA integrity among different samples to determine how PCR results might be affected.

Reverse Transcription Control Assay
Use the PrimePCR reverse transcription control assay to qualitatively assess the performance of the reverse transcription reaction associated with a single sample. This assay may also be used to compare the relative performance of the reverse transcription reactions associated with different samples.

Reference Gene Assays
Reference genes are used in relative gene expression analysis to normalize for variation in the amount of input messenger RNA (mRNA) among samples. To ensure accurate quantitation, it is important to include one or more reference genes exhibiting constant expression levels under the experimental conditions. To streamline reference gene selection, we offer PCR primers for a set of commonly used reference genes that can be used individually, easily screened using our preplated 96-well and 384-well reference panels or added to custom-designed plates.

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This locus has a highly complex imprinted expression pattern. It gives rise to maternally paternally and biallelically expressed transcripts that are derived from four alternative promoters and the 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons and this DMR is commonly found in imprinted genes and correlates with transcript expression. This gene has an antisense transcript. One of the transcripts produced from this locus and the antisense transcript are both paternally expressed noncoding RNAs and may regulate imprinting in this region. In addition one of the transcripts contains a second overlapping ORF which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed which results in different forms of the stimulatory G-protein alpha subunit a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene but the full-length nature and/or biological validity of some variants have not been determined. [provided by RefSeq Apr 2009]

Number	Description	Download
10000143205	SARS-CoV-2 Variant Detection RT-PCR Assay Protocol	Click to download
3226	PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays	Click to download
10000147102	PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol	Click to download

Sensitivity	Accurate detection of 20 copies
Specificity	Amplicon sequence validated with next generation sequencing (NGS). Minimal primer dimer formation and genomic DNA cross reactivity.
Amplification Efficiency	90–110%
Linear Dynamic Range	Minimum of six orders of magnitude. Detection of a synthetic template standard curve from 20 to 20 million copies.
R²	>0.99