Gene:  DIO1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 33'5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). Alternative splicing results in multiple transcript variants encoding different isoforms. Some but not all isoforms contain a selenocysteine (Sec) residue encoded by the UGA codon which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure the sec insertion sequence (SECIS) which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Additional transcript variants have been described but are not supported by experimental evidence. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: DIO1, Human
deiodinase, iodothyronine, type I

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0037912
Info:   Same primer pair as used in probe assay qHsaCEP0040635
List Price:    $142.00
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PrimePCR™ Probe Assay: DIO1, Human
deiodinase, iodothyronine, type I

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0040635
List Price:    $208.00
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