Gene:  A1CF, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq Nov 2010]

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PrimePCR™ SYBR® Green Assay: A1CF, Human
APOBEC1 complementation factor

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0017775
Info:   Same primer pair as used in probe assay qHsaCIP0031453
List Price:    $138.00
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PrimePCR™ Probe Assay: A1CF, Human
APOBEC1 complementation factor

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0031453
List Price:    $202.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: A1CF, Human
APOBEC1 complementation factor

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5022767
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0017775; Intron-spanning
List Price:    $138.00
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PrimePCR™ ddPCR™ Expression Probe Assay: A1CF, Human
APOBEC1 complementation factor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5056070
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0031453; Intron-spanning
 
Fluorophore:   FAM
List Price:    $207.00
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PrimePCR™ ddPCR™ Expression Probe Assay: A1CF, Human
APOBEC1 complementation factor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5056071
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0031453; Intron-spanning
 
Fluorophore:   HEX
List Price:    $207.00
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PrimePCR™ ddPCR™ Mutation Assay:A1CF p.E326G, Human
APOBEC1 complementation factor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501958
Info:   65nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:A1CF WT for p.E326G, Human
APOBEC1 complementation factor

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501959
Info:   65nt amplicon; exonic; in silico
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