PrimePCR™ Probe Assay: Nup153, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0047623
Assay Design:   Intron-spanning
Chromosome Location:   17:19560590-19565510question
Amplicon Length:   114
Splice Variants Targeted:   ENSRNOT00000001979

Gene Information

nuclear pore complex protein; may act to to gate transcribable genes to nuclear pore complexes [RGD, Feb 2006]

Gene Symbol:   Nup153
Gene Name:   Nuclear pore complex protein Nup153
Aliases:   Not Available
RefSeq:   NM_001100470
Ensembl:   ENSRNOG00000001456
Entrez:   25281
Chromosome Mapping:   17p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 38.190000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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