PrimePCR™ Probe Assay: Cacna1b, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0046925
Assay Design:   Intron-spanning
Chromosome Location:   3:1879017-1880470question
Amplicon Length:   143
Splice Variants Targeted:   ENSRNOT00000006162 ENSRNOT00000048945 ENSRNOT00000045678 ENSRNOT00000042726 ENSRNOT00000046315 ENSRNOT00000047854

Gene Information

Description Not Available

Gene Symbol:   Cacna1b
Gene Name:   Voltage-dependent N-type calcium channel subunit alpha-1B isoform 2
Aliases:   Not Available
RefSeq:   NM_147141 NM_001195199
Ensembl:   ENSRNOG00000004560
Entrez:   257648
UniGene:   Rn.85880
Chromosome Mapping:   3p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.420000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download