PrimePCR™ Probe Assay: Tmprss11c, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0036197
Assay Design:   intron_spanning
Chromosome Location:   14:23360920-23362048question
Amplicon Length:   103
Splice Variants Targeted:   ENSRNOT00000029805

Gene Information

member of the serine protease family [RGD, Feb 2006]

Gene Symbol:   Tmprss11c
Gene Name:   Transmembrane protease, serine 11c
Aliases:   Not Available
RefSeq:   NM_001003979
Ensembl:   ENSRNOG00000033910
Entrez:   408213
UniGene:   Rn.154795
Chromosome Mapping:   14p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.310000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download