PrimePCR™ Probe Assay: Smpd3, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Smpd3, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Smpd3, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0026789
Assay Design:   intron_spanning
Chromosome Location:   19:49121194-49191098question
Amplicon Length:   194
Splice Variants Targeted:   ENSRNOT00000000274 ENSRNOT00000031977 ENSRNOT00000026719 ENSRNOT00000026707

Gene Information

mRNA accumulation occurs in a growth arrested confluent monolayer but not in subconfluent cells; may play a role in regulation of cell growth [RGD, Feb 2006]

Gene Symbol:   Smpd3
Gene Name:   Sphingomyelin phosphodiesterase 3
Aliases:   Not Available
RefSeq:   NM_053605
Ensembl:   ENSRNOG00000000257
Entrez:   94338
Chromosome Mapping:   19q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.450000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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