PrimePCR™ Probe Assay: Shank3, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $208.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0026118
Assay Design:   intron_spanning
Chromosome Location:   7:130184644-130190474question
Amplicon Length:   64
Splice Variants Targeted:   ENSRNOT00000047960 ENSRNOT00000045913 ENSRNOT00000071741 ENSRNOT00000075078 ENSRNOT00000071484

Gene Information

may be involved in the structural and functional organization of the post synaptic density [RGD, Feb 2006]

Gene Symbol:   Shank3
Gene Name:   SH3 and multiple ankyrin repeat domains protein 3
Aliases:   Not Available
RefSeq:   NM_021676
Ensembl:   ENSRNOG00000032936
Entrez:   59312
UniGene:   Rn.42876
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.550000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download