PrimePCR™ Probe Assay: Oas1a, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0025882
Assay Design:   intron_spanning
Chromosome Location:   12:43069296-43072860question
Amplicon Length:   199
Splice Variants Targeted:   ENSRNOT00000027897 ENSRNOT00000000223 ENSRNOT00000067176 ENSRNOT00000057279 ENSRNOT00000038426 ENSRNOT00000048450 ENSRNOT00000027337 ENSRNOT00000050752

Gene Information

plays a role in interferon-induced antiviral response [RGD, Feb 2006]

Gene Symbol:   Oas1a
Gene Name:   2'-5'-oligoadenylate synthase 1A
Aliases:   Not Available
RefSeq:   NM_138913
Ensembl:   ENSRNOG00000001369
Entrez:   192281
Chromosome Mapping:   12q16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 37.510000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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