PrimePCR™ Probe Assay: Bckdha, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0025876
Assay Design:   intron_spanning
Chromosome Location:   1:83692945-83696205question
Amplicon Length:   64
Splice Variants Targeted:   ENSRNOT00000027995

Gene Information

plays a role in the conversion of 3-methyl-2-oxobutanoate and lipoamide to S-(2-methylpropanoyl)dihydrolipoamide and CO2 [RGD, Feb 2006]

Gene Symbol:   Bckdha
Gene Name:   2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
Aliases:   Not Available
RefSeq:   NM_012782
Ensembl:   ENSRNOG00000020607
Entrez:   25244
UniGene:   Rn.49145
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.610000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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