PrimePCR™ Probe Assay: Rela, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0025841
Assay Design:   intron_spanning
Chromosome Location:   1:227931906-227936069question
Amplicon Length:   95
Splice Variants Targeted:   ENSRNOT00000045233

Gene Information

component of the NF-kappa B complex; involved in transcriptional activation [RGD, Feb 2006]

Gene Symbol:   Rela
Gene Name:   Transcription factor p65
Aliases:   Not Available
RefSeq:   NM_199267
Ensembl:   ENSRNOG00000030888
Entrez:   309165
UniGene:   Rn.19480
Chromosome Mapping:   1q43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.890000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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