PrimePCR™ Probe Assay: Cav1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0025444
Assay Design:   intron_spanning
Chromosome Location:   4:45205047-45234288question
Amplicon Length:   118
Splice Variants Targeted:   ENSRNOT00000009253

Gene Information

structural protein of lipid raft domains in the membrane called caveolae; functions as a cholesterol-binding/shuttling protein [RGD, Feb 2006]

Gene Symbol:   Cav1
Gene Name:   Caveolin-1 alpha isoform
Aliases:   Not Available
RefSeq:   NM_031556 NM_133651
Ensembl:   ENSRNOG00000006694
Entrez:   25404
UniGene:   Rn.22518
Chromosome Mapping:   4q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 37.440000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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