PrimePCR™ Probe Assay: Pde3b, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0025203
Assay Design:   intron_spanning
Chromosome Location:   1:191027621-191033181question
Amplicon Length:   112
Splice Variants Targeted:   ENSRNOT00000015498

Gene Information

catalyzes the hydrolysis of cAMP and cGMP; regulates insulin secretory processes [RGD, Feb 2006]

Gene Symbol:   Pde3b
Gene Name:   CGMP-inhibited 3',5'-cyclic phosphodiesterase B
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000011417
Entrez:   29516
UniGene:   Rn.10322
Chromosome Mapping:   1q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.280000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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