PrimePCR™ Probe Assay: Ada, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0023123
Assay Design:   intron_spanning
Chromosome Location:   3:166312215-166314628question
Amplicon Length:   114
Splice Variants Targeted:   ENSRNOT00000014151

Gene Information

catalyzes the conversion of adenosine to inosine in adenosine metabolism; may act as a neuormodulator in sleep-wake regulation [RGD, Feb 2006]

Gene Symbol:   Ada
Gene Name:   Adenosine deaminase
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000010265
Entrez:   24165
UniGene:   Rn.12689
Chromosome Mapping:   3q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.720000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download