PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000046835, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000046835, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000046835, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0020832
Assay Design:   intron_spanning
Chromosome Location:   14:908443-916385question
Amplicon Length:   89
Splice Variants Targeted:   ENSRNOT00000001397 ENSRNOT00000042693 ENSRNOT00000073754 ENSRNOT00000034630 ENSRNOT00000074428 ENSRNOT00000073410 ENSRNOT00000044505 ENSRNOT00000074130 ENSRNOT00000073032 ENSRNOT00000052336 ENSRNOT00000050676 ENSRNOT00000049917 ENSRNOT00000045104 ENSRNOT00000049544 ENSRNOT00000039900 ENSRNOT00000044004 ENSRNOT00000052140 ENSRNOT00000072859 ENSRNOT00000071182 ENSRNOT00000073546 ENSRNOT00000045627 ENSRNOT00000050254 ENSRNOT00000051726 ENSRNOT00000048404 ENSRNOT00000044270 ENSRNOT00000049921 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000046835
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046835
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.950000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download