PrimePCR™ SYBR® Green Assay: FCGR2A, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FCGR2A, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FCGR2A, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0019003
Assay Design:   intron_spanning
Chromosome Location:   13:95738735-95740680question
Amplicon Length:   91
Splice Variants Targeted:   ENSRNOT00000074149 ENSRNOT00000045427 ENSRNOT00000058496 ENSRNOT00000036799 ENSRNOT00000071728 ENSRNOT00000047349 ENSRNOT00000071897 ENSRNOT00000004204 ENSRNOT00000073643 ENSRNOT00000009121 ENSRNOT00000027237 ENSRNOT00000065229 ENSRNOT00000070912 ENSRNOT00000068489

Gene Information

Description Not Available

Gene Symbol:   FCGR2A
Gene Name:   LOC498276 protein; Protein LOC498276
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050418
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.360000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download