PrimePCR™ SYBR® Green Assay: Plekha7, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0017386
Assay Design:   intron_spanning
Chromosome Location:   1:192453650-192457519question
Amplicon Length:   117
Splice Variants Targeted:   ENSRNOT00000064706

Gene Information

Description Not Available

Gene Symbol:   Plekha7
Gene Name:   Pleckstrin homology domain containing, family A member 7
Aliases:   Not Available
RefSeq:   NM_001144861
Ensembl:   ENSRNOG00000024602
Entrez:   499249
UniGene:   Rn.202099
Chromosome Mapping:   1q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.850000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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