PrimePCR™ SYBR® Green Assay: Ppp1r17, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCID0014908
Assay Design:   intron_spanning
Chromosome Location:   4:150933617-150937274question
Amplicon Length:   111
Splice Variants Targeted:   ENSRNOT00000016414

Gene Information

substrate of the cGMP-dependent protein kinase and is involved in the inhibition of PP-1 and PP-2A phosphatases [RGD, Feb 2006]

Gene Symbol:   Ppp1r17
Gene Name:   G-substrate
Aliases:   Not Available
RefSeq:   NM_153467
Ensembl:   ENSRNOG00000012235
Entrez:   266705
UniGene:   Rn.24323
Chromosome Mapping:   4q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 36.090000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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